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如題,文章大修一個審稿人提了如下問題,請問我該如何回答,謝謝! 這個問題是針對我做的定量PCR來提的。 I don't think authors have followed the MIQE Guidelines for carrying out the qPCR experiments and also in presenting the data, this is a major concern. qPCR information need to be updated in the Results and discussion section as well. Please go through below literature before updating the content. Bustin, S.A., Beaulieu, J., Huggett, J. et al. MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments. BMC Molecular Biol 11, 74 (2010) doi: 10.1186/1471-2199-11-74 The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments Stephen A. Bustin, Vladimir Benes, Jeremy A. Garson, Jan Hellemans, Jim Huggett, Mikael Kubista, Reinhold Mueller, Tania Nolan, Michael W. Pfaffl, Gregory L. Shipley, Jo Vandesompele, Carl T. Wittwer Clinical Chemistry Apr 2009, 55 (4) 611-622; DOI: 10.1373/clinchem.2008.112797” |
至尊木蟲 (職業(yè)作家)
新蟲 (正式寫手)
至尊木蟲 (職業(yè)作家)
新蟲 (正式寫手)
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