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題目 Cellular and Subcellular Sites for Noradrenergic Action in the Monkey Dorsolateral Prefrontal Cortex as Revealed by the Immunocytochemical Localization of Noradrenergic Receptors and Axons 摘要 A series of electron microscopic immunocytochemical studies was performed to analyze subcellular sites for noradrenergic modulation in monkey prefrontal cortex. One out of 12 noradrenergic varicosities, identified by dopamine b-hydroxylase immunocytochemistry within single ultrathin sections, forms morphologically identifiable junctions with small dendrites and spines. Accordingly, a2-adrenergic receptors, almost all of which are of the A-subtype, that occur in spines are localized discretely over postsynaptic membranes. a2-Adrenergic receptors are also found at sites along axons, dendritic shafts and astrocytic processes lacking morphologically identifiable synaptic junctions, suggesting that these receptors are activated by volume transmission. In particular, axonal a2-adrenergic receptors occur mostly at pre-terminal regions, suggesting that axo-axonic interactions may mediate reduction of neurotransmitter release at sites other than axo-spinous junctions by closing voltage-dependent calcium channels. These results indicate that noradrenergic modulation of prefrontal cortex involves synaptic interactions at spines of pyramidal neurons and nonsynaptic volume transmission to glia, dendritic shafts and axons. |

至尊木蟲 (知名作家)
Translator and Proofreader
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用猴背外側(cè)前額葉皮層去甲腎上腺素能受體和軸突的免疫細(xì)胞化學(xué)定位確立去甲腎上腺素的細(xì)胞和亞細(xì)胞作用位點(diǎn) 摘要: 用一系列電子顯微鏡免疫細(xì)胞化學(xué)研究,分析了猴前額葉皮質(zhì)去甲腎上腺素能調(diào)制的亞細(xì)胞位點(diǎn)。采用多巴胺β-羥化酶免疫細(xì)胞化學(xué)發(fā)現(xiàn),在單一的超薄切片內(nèi)每12個(gè)去甲腎上腺素能膨體中就有一個(gè)和小的樹突棘形成在形態(tài)上可見的接頭。與此相應(yīng),α2 - 腎上腺素能受體幾乎都屬于A亞型,在神經(jīng)嵴都散在分布于突觸后膜。此外,α2-腎上腺素能受體也存在于沿著軸突、樹突狀軸和星形細(xì)胞過程缺乏形態(tài)上可見的突觸接頭等位點(diǎn),表明這些受體是通過容積傳遞方式激活的。特別是,軸突部位的α2-腎上腺素能受體主要存在與前突觸區(qū)域,提示除了在軸突-棘突接頭之外,可以通過軸突與軸突間的相互作用,關(guān)閉電壓依賴性鈣通道,使神經(jīng)遞質(zhì)釋放的減少。這些結(jié)果表明,去甲腎上腺素對前額葉皮層的調(diào)制作用包括在錐體神經(jīng)元之神經(jīng)嵴部位的突觸間相互作用和對神經(jīng)膠質(zhì)細(xì)胞、樹突狀軸、以及軸突的非突觸性容積傳遞。 |
木蟲 (正式寫手)

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