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jiushengming金蟲 (正式寫手)
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請教大神,這段話如何翻譯? 不勝感激! DHT是一種弱酸性物質,HPLC 分析時在流動相中加入一定量的酸可以減少色譜峰的拖尾并增加對稱度。 本實驗采用甲酸和乙酸。 流動相中甲酸和乙酸添加量對DHT對映體分離的影響列于表 3。 當流動相中不添加甲酸和乙酸時,DHT的兩個對映體可以達到基線分離。鑒于柱子的耐用性,流動相中酸添加量在 0. 1% ~ 0.2% 之間時, DHT對映體可同時實現(xiàn)基線分離。 為獲得較好的分離效果,并盡量較少保留時間,最終選擇添加0.2%甲酸。 |
至尊木蟲 (著名寫手)
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樓主,不加酸兩個異構體是不能分開的是嗎? DHT是一種弱酸性物質,HPLC 分析時在流動相中加入一定量的酸可以減少色譜峰的拖尾并增加對稱度。 本實驗采用甲酸和乙酸。 流動相中甲酸和乙酸添加量對DHT對映體分離的影響列于表 3。 As DHT is a weak acid substances, adding a certain amount of acid to the mobile phase for HPLC analysis can reduce the tailing of the peak and increase the symmetry of the peak. In the experiments formic acid and acetic acid were used. The effects of formic acid and acetic acid on the separation of DHT enantiomers were listed in Table 3. 當流動相中不添加甲酸和乙酸時,DHT的兩個對映體可以達到基線分離。鑒于柱子的耐用性,流動相中酸添加量在 0. 1% ~ 0.2% 之間時, DHT對映體可同時實現(xiàn)基線分離。 為獲得較好的分離效果,并盡量較少保留時間,最終選擇添加0.2%甲酸。When the separation was conducted using a mobile phase without formic acid and acetic acid, a baseline separation could (not?) be achieved for the two enantiomers of DHT. In view of not to affect the durability of the column, when the acid content was between 0.1% ~ 0.2% in the mobile phase, DHT enantiomers could simultaneously achieve baseline separation. To achieve better separation results, and to reduce the retention time as short as possible, finally we chose to add 0.2% formic acid. |

至尊木蟲 (著名寫手)
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DHT was a weak acidic material. Adding certain amount of acid (formic acid and acetic acid were used in this experiment) into mobile phase in HPLC analysis chould reduce tailed chromatographic peaks and increase its degree of symmetry. The effects of the additive amount of formic acid and acetic acid in mobile phase to DHT enantiomeric separation were listed in table 3. The two DHT enantiomorphs chould achieve baseline separation, without no acid(formic acid and acetic acid) addition in mobile phase. Considering the durability of column, DHT enantiomorphs were simultaneous achieved baseline separation when acid addition was 0. 1% ~ 0.2%. Furthermore, 0.2% formic acid addition was final chose to obtain better separation effect and less retention time simultaneously. |

至尊木蟲 (著名寫手)

金蟲 (正式寫手)
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