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| 各位朋友,請幫忙!急求文章Enhancement of L-phenylalanine production by engineered Escherichia coli using phased exponential L-tyrosine feeding combined with nitrogen source optimization 的出版號,即ISI號。拜托。! |
版主 (文壇精英)
有尾巴的青蛙
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FN Thomson Reuters Web of Science™ VR 1.0 PT J AN 25553973 DT Journal Article TI Enhancement of l-phenylalanine production by engineered Escherichia coli using phased exponential l-tyrosine feeding combined with nitrogen source optimization. AU Yuan, Peipei Cao, Weijia Wang, Zhen Chen, Kequan Li, Yan Ouyang, Pingkai SO Journal of bioscience and bioengineering VL 120 IS 1 PS 36-40 PY 2015 PD 2015 Jul (Epub 2014 Dec 29) LA English AB Nitrogen source optimization combined with phased exponential l-tyrosine feeding was employed to enhance l-phenylalanine production by a tyrosine-auxotroph strain, Escherichia coli YP1617. The absence of (NH4)2SO4, the use of corn steep powder and yeast extract as composite organic nitrogen source were more suitable for cell growth and l-phenylalanine production. Moreover, the optimal initial l-tyrosine level was 0.3gL(-1) and exponential l-tyrosine feeding slightly improved l-phenylalanine production. Nerveless, l-phenylalanine production was greatly enhanced by a strategy of phased exponential l-tyrosine feeding, where exponential feeding was started at the set specific growth rate of 0.08, 0.05, and 0.02h(-1) after 12, 32, and 52h, respectively. Compared with exponential l-tyrosine feeding at the set specific growth rate of 0.08h(-1), the developed strategy obtained a 15.33% increase in l-phenylalanine production (l-phenylalanine of 56.20gL(-1)) and a 45.28% decrease in l-tyrosine supplementation. Copyright 2014 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved. C1 State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 211816, People's Republic of China. SS Index Medicus ID Engineered Escherichia coli; Fed-batch; Nitrogen source; l-Phenylalanine; l-Tyrosine feeding SN 1347-4421 JC 100888800 PA Japan SA In-Data-Review RC 05 Jun 2015 PE 29 Dec 2014 DI 10.1016/j.jbiosc.2014.12.002 UT MEDLINE:25553973 ER EF |

版主 (文壇精英)
有尾巴的青蛙

版主 (文壇精英)
有尾巴的青蛙

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