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本實(shí)驗(yàn)室 （Multiplex Biotechnology Laboratory at SUNY Albany）招收一名全額獎(jiǎng)學(xué)金資助的化學(xué)博士研究生。研究項(xiàng)目包括生物醫(yī)學(xué)芯片和單細(xì)胞研究。研究方向是利用分析化學(xué)，生物工程和計(jì)算機(jī)等基本技術(shù)來解決一些重大的癌癥生物學(xué)，免疫治療，傳染病等問題。申請人的背景可以是化學(xué)，生物工程，生物醫(yī)學(xué)，或是其他生物化學(xué)學(xué)科。申請人之前的研究內(nèi)容和方向不重要, 但因?yàn)槁毼辉诨瘜W(xué)系所以最好能通過基本的化學(xué)課程。實(shí)驗(yàn)室PI 本身就是生物工程背景，所以化學(xué)環(huán)境不會(huì)阻擋你的發(fā)展。希望申請人有扎實(shí)的學(xué)科基礎(chǔ)并且有一定的動(dòng)手能力。入學(xué)時(shí)間為2016年秋季。春季或夏季提前入學(xué)也有可能。 有興趣的申請人請發(fā)信給 jwang34@albany.edu. 歡迎來信咨詢。如下是本實(shí)驗(yàn)室網(wǎng)址： https://www.multiplexbiotechnologylab.com/ PhD Research Assistant in Chemistry We are seeking a highly motivated Research Assistant in Analytical Chemistry or Biochemistry, starting immediately in the Multiplex Biotechnology Laboratory. The potential student will work on research projects related to biomedical microdevice and single-cell systems biology for disease diagnosis and treatment. Research directions include exploring analytical chemistry, bioengineering and computational solutions for emerging problems in cancer biology, immunotherapy, infectious disease, and regenerative medicine. The student with Chemistry, Bioengineering, Biomedical Science/Engineering, and Systems Biology backgrounds are preferred, who should have demonstrated ingenuity, productivity, and ability to work in a diverse, high energy environment. Prior research experience for this position is not required, and a master degree is preferred but not required. Interested candidates should send a letter describing your interest in the position, CV, and names of references to Email: jwang34@albany.edu. Evaluation of candidates will begin immediately and will continue until these positions are filled. For any questions, please contact Dr. Jun Wang, Assistant Professor, SUNY Albany. Website: https://www.multiplexbiotechnologylab.com/ 要是有人上不網(wǎng)站，可以看以下內(nèi)容： ------------------------------------------------------------------------------------------------------ Research 1. Multiplex biosensor We are developing innovative sensing platforms for the multiplexed detection of protein biomarkers and DNAs. These platforms are based on barcoded, free-standing microparticles, similar to the elements of conventional microarrays. The special encoding methods permit the production of unlimited amount of uniquely coded microparticles for wide applications in medical science, forensics, defense and pharmaceutical industry. 2. Single-cell signaling Signaling transduction in any cells always involves a number of signaling proteins. How they interact and cooperate with each other is varied dramatically between different cell types and even in the same population. We developed a single-cell microchip technology before that uniquely profiles all the signaling proteins including secreted proteins, activated membrane receptors and phosphoproteins from single cancer cells and immune cells. The two directions in our lab are as follows: 1. Real-time detection of signaling pathway activities in single live cells. Conventional approaches by GFP labeling etc. are limited by the number of multiplexity and are not powerful enough for signaling pathway studies in live cells. We are developing micro-/nano- technologies to probe the activities of multiple signaling pathways from the same live single cells. 2. Point-of-care single-cell diagnostics tools. Although cellular heterogeneity has been well acknowledged, multiplexed single-cell tools are still a luxury in diagnostics and are not commonly used like western blot and ELISA. We are developing unified single-cell tools to profile a number of signaling proteins at the cost of $1 in a field setting for anyone without special training. 3. Systems biology technologies The technology in our lab can incorporate both proteomic and transcriptomic studies in one platform. With our unique single-cell technology, we are trying to understand the fundamental biological phenomena such as how cancer cells and immune cells are communicating and living in the same tumor, why the immune cells are so diversified, how to find the right target to kill all cancer cells, etc. Publication 28. Li, B., He, MH, Ramirez, L.S., George, J., and Wang, J.# Multifunctional Hydrogel Microparticles by Polymer-Assisted Photolithography. Submitted. 27. Ramirez, L.S.,* He, MH,* Mailloux, S., George, J., and Wang, J.# Microfabrication of DNA-grafted QR Barcode Microparticles. Submitted. (*co-first author) 26. Mailloux, S., Ramirez, L.S., and Wang, J.# Microfluidic Single-Cell Functional Proteomics. In the book Microfluidic Methods for Molecular Biology. Springer, 2015. 25. Ramirez, L.S. and Wang, J.# Flow-Pattern Guided Fabrication of High-Density Barcode Antibody Microarray. Journal of Visualized Experiments, (2015), e53644, doi: 10.3791/53644. (Invited article) Before 2014 (Caltech and Purdue University) 24. Wang, J.*, Kravchenko-Balasha, N.*, Levine, R. D, Heath, J. R. Glioblastoma cellular architectures are predicted through knowledge of two-cell interactions. Proceedings of National Academy of Sciences 111(2014) 6521-6526. (IF=9.7; *co-first author) 23. Nathanson, D., Gini, B., Mottahedeh, J., Visnyei, K., Koga, T., Gomez, G., Eskin, A., Hwang, K., Wang, J., et al. Targeted therapy resistance mediated by dynamic regulation of extrachromosomal mutant EGFR DNA. Science, 343(2014) 72-76. (IF=31.0) 22. Wei, W., Shin, Y. S., Ma, C., Wang, J., Elitas, M., Fan, R., Heath, J.R. Microchip platforms for multiplex single-cell functional proteomics with applications to immunology and cancer research. Genome Medicine, 5(2013) 75-81. (IF=3.9) 21. Dooraghi, A. A., Vu, N. T., Silverman, R. W., Farrell, R., Shah, K. S., Wang, J., Heath, J. R. and Chatziioannou, A. F. Betabox: a beta particle imaging system based on a position sensitive avalanche photodiode. Physics in Medicine and Biology, 58(2013) 3739-3753. (IF=2.8) 20. Wang, J., Tham, D., Ahmad, H., Ma, C., Yu, J.-K., Heath, J. R. Quantitating cell-cell interaction functions, with applications to glioblastoma multiforme cancer cells. Nano Letters, 12(2012) 6101-6106. (IF=13.1) - "Listen to Cancer Cell chat" Chemical & Engineering News (November,2012). 19. Wang, J., Kwang, K.W., Brass, D., Radu, C., Christofk, H., Phelps, M., Heath, J. R. Fast metabolic response to drug intervention through analysis on a miniaturized, highly integrated molecular imaging system. Journal of Nuclear Medicine, 54(2013) 1820-1824. (IF=6.4) 18. Zhan, Y., Cao, Z., Bao, N., Li, J., Wang, J., Geng, T., Lin, H., Lu, C. Low-frequency ac electroporation shows strong frequency dependence and yields comparable transfection results to dc electroporation. Journal of Controlled Release 160 (2012) 570-576. (IF=6.5) 17. Wang, J.*, Zhan, Y.*, Bao, N., Lu, C. Quantitative measurement of quantum dot uptake at the cell population level using microfluidic evanescent-wave-based flow cytometry. Lab on a Chip, 12 (2012) 1441-1445. (IF=6.3; * co-first author) - Cover article in Issue 8 of Lab on a Chip. 16. Geng, T., Zhan, Y., Wang, J., Lu, C. Transfection of cells using flow-through electroporation based on constant voltage. Nature Protocols 6 (2011) 1192-1208. (IF=9.9) 15. Vermesh, U*., Vermesh, O.*, Wang, J., Kwong, G. A., Ma, C., Hwang, K., Heath, J. R. High-density, multiplexed patterning of cells at single-cell resolution for tissue engineering and other applications. Angewandte Chemie International Edition 32 (2011) 7378-7380. (IF=13.5) 14. Wang, J., Ahmad, H., Ma, C., Shi, Q. -H., Vermesh, O., Vermesh, U. and Heath, J. R. A self-powered, one-step chip for rapid, quantitative and multiplexed detection of proteins from pinpricks of whole blood. Lab on a Chip 10 (2010) 3157-3162. (IF=6.3) 13. Wang, J., Zhan, Y., Ugaz, V. M and Lu, C. Vortex-assisted DNA delivery. Lab on a Chip 10 (2010) 2057-2061. (IF=6.3) - Cover article in Issue 16 of Lab on a Chip. - "Biotechnology: Swirling cells." Nature (Vol 466, July 8 2010, page 163) 12. Wang, J., Fei, B., Zhan, Y., Geahlen, R. L. and Lu, C. Kinetics of NF-kappaB nucleocytoplasmic transport probed by single-cell screening without imaging. Lab on a Chip 10 (2010) 2911-2916. (IF=6.3) 11. Wang, J., Fei, B., Geahlen, R. L. and Lu, C. Quantitative analysis of protein translocations by microfluidic total internal reflection fluorescence flow cytometry. Lab on a Chip 10 (2010) 2673-2679. (IF=6.3) 10. Zhan, Y., Wang, J., Bao, N., and Lu, C. Electroporation of cells in microfluidic droplets. Analytical Chemistry 81 (2009) 2027-2031. (IF=5.8) 9. Wang, J., Bao, N., Paris, L. L., Geahlen, R. L. and Lu, C. Total internal reflection fluorescence flow cytometry. Analytical Chemistry 80 (2008) 9840-9844. (IF=5.8) 8. Wang, J., Bao, N., Paris, L. L., Wang, H. -Y., Geahlen, R. L. and Lu, C. Detection of kinase translocation using microfluidic electroporative flow cytometry. Analytical Chemistry 80 (2008) 1087-1093. (IF=5.8) 7. Bao, N., Wang, J. and Lu, C. Recent advances in electric analysis of cells in microfluidic systems. (Invited review) Analytical and Bioanalytical Chemistry 391 (2008) 933-942. (IF=3.8) 6. Bao, N., Wang, J. and Lu, C. Microfluidic electroporation for selective release of intracellular molecules at the single cell level. Electrophoresis 29 (2008) 2939-2944. (IF=3.3) 5. Wang, F., Wang, H., Wang, J., Wang, H. Y., Rummel, P. L., Garimella, S. V. and Lu, C. Microfluidic delivery of small molecules into mammalian cells based on hydrodynamic focusing. Biotechnology and Bioengineering 100 (2008) 150-158. (IF=3.9) - Selected as the cover article (Vol.100 Issue 1). 4. Wang, J., Stine, M. J. and Lu, C. Microfluidic cell electroporation using a mechanical valve. Analytical Chemistry 79 (2007) 9584-9587. (IF=5.8) 3. Wang, J. and Lu, C. Single molecule λ-DNA stretching studied by microfluidics and single particle tracking. Journal of Applied Physics 102 (2007) 074703. (IF=2.2) 2. Wang, J. and Lu, C. Microfluidic cell fusion under continuous direct current voltage. Applied Physics Letters 89 (2006) 234102. (IF=3.8) 1. Wang, J., Xu, Z. -N.# , Shen, W. -H. and Cen, P. -L. Study on the extraction equilibrium of tilmicosin between aqueous and butyl acetate phases. Chemical Engineering Communications, 193 (2006) 427-437. (# professor; IF=0.9) * Equal contribution; IF: impact factor Principal Investigator Jun Wang, Ph.D. Assistant Professor of Chemistry Postdoc. California Institute of Technology, 2014 Ph.D. Purdue University, 2010 Biography Jun Wang is an Assistant Professor in the Department of Chemistry at SUNY Albany. Prior to joining SUNY Albany, he received postdoctoral training in the Department of Chemistry and the NanoSystems Biology Cancer Center at the California Institute of Technology. His research in Prof. James Heath's group includes one-step, self-powered blood chip and 2D barcode microarray for single-cell proteomics. He discovered that cell communications make cancer cells more heterogeneous and cause their resistance to targeted drug treatment. He obtained his Ph.D. in Biological Engineering from Purdue University where his research in Prof. Chang Lu's group was focused on single-cell microfluidics and gene delivery. He employed electroporation to differentially release intracellular proteins and invented the first flow cytometry that sorts cells according to the subcellular location of signaling proteins. He has received several top awards including Chorafas Foundation prize. Email: jwang34 at albany dot edu Visiting Scholar Fan Yang, Ph.D. Ph.D. Sun Yat-sen University B.S. Hubei University of Chinese Medicine Fan joined the MBL group with the desire to design and engineer novel biosensors toward disease diagnostics. He often pays curiosity on what happens on the sensing interface, and he is interested in the development of point-of-care diagnostic device by combining micro- and nanoscale technologies. He spends his free time in reading, travelling and watching movies. Email: fyang3 at albany dot edu Postdoctoral Fellows Bin Li, Ph.D. Ph.D. Changchun Institute of Applied Chemistry, 2014 B.S. Anhui University, 2009 Bin joined the MBL team with the goal to integrate polymer science into microarray fabrication. He is interested in design of sophisticated arrays to detect what happens in the nano world. Bin shows rationality in work, and sensibility in life. He likes cooking, travelling and any other delightful experience for his spare time. Email: bli5 at albany dot edu Meihua Lin, Ph.D. Ph.D. Shanghai Institute of Applied Physics, 2015 B.S. Fuzhou University, 2010 Meihua is currently focusing on real-time detection of secreted proteins in single cell. When she is not in the laboratory, she spends her free time on reading, cooking and watching TV series. Email: mlin at albany dot edu Graduate Students Muhan(Leslie) He B.S. Southwest University for Nationalities Leslie is interested in research about cancer cell communications and the possible drug targets to inhibit growth and metastasis of tumor cells, utilizing microarray and microfluidics. For her free time, she likes reading crime story and watching TV series. Her favorite TV series is Supernatural. Email: mhe6 at albany dot edu Lisa Ramirez B.S. University of the Philippines, 2012 Lisa is interested in developing microarrays for studying cell-cell interactions. When she is not in the laboratory, she spends her free time cooking, baking, and reading movie critiques. Email: lsramirez at albany dot edu Research Technician Qiu Gao B.S. SUNY Buffalo, 2015 Qiu is interested in the health related field especially how different disorder could arise from the same source. He believed doing simple daily task could improve one's health like drinking water over soda. On his free time likes going to the gym, hiking, or snowboarding. Undergraduate Students Justin George Chemistry, SUNY Albany (2012- ) Email: jvgeorge at albany dot edu Justin is a NYS EMT-B for the Five Quad Volunteer Ambulance on campus. In his free time Justin plays the French Horn in the University’s Symphonic Band and is a member of the UAlbany Running Exchange. His favorite T.V shows to watch are Arrow, Flash, and Criminal Minds. He loves to eat at Chipotle. “The burritos are the best in town!” This is his first time in a research lab, and he is very excited! Brittany Egnot Bioengineering, SUNY Albany & SUNY PI ( with Dr. Janet Paluh) Brittany is a junior at UAlbany/CNSE studying bioengineering and nanoscale science. She is interested in nanoscale cell-cell interactions and their clinical applications. After completing her undergraduate curriculum, Brittany hopes to enter an MD/PhD program and study translational medicine with an emphasis on cell differentiation and regenerative therapies. Brittany was named Distinguished Young Woman of New York in 2013 and is passionate about science outreach, performing jazz piano and vocal standards, and trying every variety of sushi in existence. [ Last edited by jwangalbany on 2016-1-11 at 12:06 ] 本實(shí)驗(yàn)室只招TA，需要TOEFL 100分以上，GRE最好有。 [ Last edited by jwangalbany on 2016-2-26 at 11:51 ] |
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