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The methylated products were hydrolyzed with 0.5 M HCl/aqueous 80% AcOH at 70 C for 0.5 h, followed by reduction and acetylation to obtain partially methylated alditol acetates (PMAAs).PMAAs were identified by their fragment ions in EI-MS and by their relative retention times in GC. Their molar ratios were estimated from the peak areas of total ion chromatograms from GC–MS. Relative molar ratios of [U–13C]PMAA from [U–13C]glucosyl residue and [U-12C]PMAA from [U-12C]glucosyl residue ([U–13C]PMAA/[U-12C]PMAA) were estimated from the peak areas of their characteristic fragment ions (m/z) detected |
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