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北京石油化工學(xué)院2026年研究生招生接收調(diào)劑公告

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17樓: Originally posted by richbird at 2014-06-19 17:34:23
代謝組學(xué)會受吸收藥物和藥物代謝的雙重影響，蛋白組學(xué)受到的影響相對較少。因為是復(fù)方，化合物成分復(fù)雜。但是對于多數(shù)藥物而言，會結(jié)合在血漿蛋白上。當(dāng)然組織器官也有有一定的蛋白結(jié)合，假如沒有特異性結(jié)合或者分布 ...

http://www.mcponline.org/content/12/5/1226.short 這是原文網(wǎng)址他的方法那些都在Supporting Information 里

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21樓2014-06-19 21:22:52

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把方法的英語原文貼出來吧，避免英語理解錯了的原因

Animal sampling and handing
The animals were randomly assigned to 10 groups of 6 rats each as follows: control, HI, YCHT, D, G, R, DG, DR, GR and DGR groups. The rats in the control group were given distilled water for 8 consecutive days and anesthetized via an intraperitoneal injection of 1% pentobarbital sodium (0.15 ml/100 g body weight) on day 9. The rats in the HI group were orally administrated 20% CCL4 (1 ml/kg body weight) for 8 consecutive days (at 6:00 p.m.), and on day 9, the rats were anesthetized as in the control group. Simultaneously, other groups were administrated CCL4 and various treatments for 9 consecutive days, then anesthetized as in the control group. The rats were orally given 1 ml/100 g each, and the doses of the three components used in this study were administered in accordance with the “Shanghanlun”. Blood was collected from the hepatic portal vein, and plasma was separated via centrifugation at 4,500 rpm for 5 min at 4°C, flash frozen in liquid nitrogen and stored at -80°C until the liver function tests and metabolomics and proteomics analyses were performed. The plasma ALT, AST, ALP, GSH-PX and SOD activities and MDA, TG, GT, CHOL, TP, D-BIL and T-BIL contents were measured according to the manufacturer’s instructions. The rat livers were removed immediately after plasma collection and stored at -70°C until analysis. Urine was collected daily (at 6:00 a.m.) from the metabolic cages at ambient temperature throughout the entire procedure and centrifuged at 10,000 rpm at 4°C for 5 min; the supernatants were then stored frozen at -20°C for subsequent metabolomic analysis.

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22樓2014-06-19 21:27:10

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19樓: Originally posted by work037 at 2014-06-19 21:00:45
還是把你看的文獻(xiàn)貼出來，我們才好知道來龍去脈，才好一同分析

Animal sampling and handing
The animals were randomly assigned to 10 groups of 6 rats each as follows: control, HI, YCHT, D, G, R, DG, DR, GR and DGR groups. The rats in the control group were given distilled water for 8 consecutive days and anesthetized via an intraperitoneal injection of 1% pentobarbital sodium (0.15 ml/100 g body weight) on day 9. The rats in the HI group were orally administrated 20% CCL4 (1 ml/kg body weight) for 8 consecutive days (at 6:00 p.m.), and on day 9, the rats were anesthetized as in the control group. Simultaneously, other groups were administrated CCL4 and various treatments for 9 consecutive days, then anesthetized as in the control group. The rats were orally given 1 ml/100 g each, and the doses of the three components used in this study were administered in accordance with the “Shanghanlun”. Blood was collected from the hepatic portal vein, and plasma was separated via centrifugation at 4,500 rpm for 5 min at 4°C, flash frozen in liquid nitrogen and stored at -80°C until the liver function tests and metabolomics and proteomics analyses were performed. The plasma ALT, AST, ALP, GSH-PX and SOD activities and MDA, TG, GT, CHOL, TP, D-BIL and T-BIL contents were measured according to the manufacturer’s instructions. The rat livers were removed immediately after plasma collection and stored at -70°C until analysis. Urine was collected daily (at 6:00 a.m.) from the metabolic cages at ambient temperature throughout the entire procedure and centrifuged at 10,000 rpm at 4°C for 5 min; the supernatants were then stored frozen at -20°C for subsequent metabolomic analysis.

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23樓2014-06-19 21:28:10

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謝謝大家得幫助了，第一次這么多回復(fù)，好感動啊

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24樓2014-06-19 21:57:38

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22樓: Originally posted by 茜草科 at 2014-06-19 21:27:10
把方法的英語原文貼出來吧，避免英語理解錯了的原因

Animal sampling and handing
The animals were randomly assigned to 10 groups of 6 rats each as follows: control, HI, YCHT, D, G, R, DG, DR, GR an ...

作者對造模藥物、治療藥物、采血具體時間三者的關(guān)系沒有交代清楚。但是作者所用的三個藥物，半衰期都不長。而且即便是采集肝門靜脈血，假如采血時間在給藥時間后幾個小時，那么血液已經(jīng)在全身經(jīng)歷了n個循環(huán)了，已經(jīng)是代謝過的血液。所以，假如僅僅是為了避開肝臟代謝，我覺得意義不大。但是血液經(jīng)過肝臟后，會發(fā)生一系列的生理變化，比如三大物質(zhì)代謝和轉(zhuǎn)運(yùn)，血漿蛋白的更新。這些變化或許對代謝組或者蛋白組發(fā)生一定影響。但是這些影響同時也間接反應(yīng)了肝臟的功能，所以個人看法是采用統(tǒng)一的沒有污染的采血方法即可，不建議眼眶采血（污染），但是其他如頸動脈插管也可以。
最后，我贊同你的觀點：對照組應(yīng)當(dāng)和治療組保持相同的給藥方法和持續(xù)時間。即便差一天可能不會產(chǎn)生非常大的影響，但是這并未得到驗證，還是存在一定風(fēng)險。
你所說的

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25樓2014-06-20 09:15:29

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25樓: Originally posted by richbird at 2014-06-20 09:15:29
作者對造模藥物、治療藥物、采血具體時間三者的關(guān)系沒有交代清楚。但是作者所用的三個藥物，半衰期都不長。而且即便是采集肝門靜脈血，假如采血時間在給藥時間后幾個小時，那么血液已經(jīng)在全身經(jīng)歷了n個循環(huán)了，已經(jīng) ...

謝謝你的幫助很有條理還有個問題想請教關(guān)于眼眶取血你指的污染是質(zhì)量不好還是容易溶血？還有之前說的預(yù)防給藥治療性給藥和同時性給藥他們的優(yōu)缺點怎么看作者幾年前一篇文章同樣課題沒研究協(xié)同作用什么的只是證明茵陳蒿有效是預(yù)防性給藥我感覺這三種給藥方式應(yīng)該在后期處理上會有些不同

[ 發(fā)自小木蟲客戶端 ]

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26樓2014-06-20 10:27:26

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26樓: Originally posted by 茜草科 at 2014-06-20 10:27:26
謝謝你的幫助很有條理還有個問題想請教關(guān)于眼眶取血你指的污染是質(zhì)量不好還是容易溶血？還有之前說的預(yù)防給藥治療性給藥和同時性給藥他們的優(yōu)缺點怎么看作者幾年前一篇文章同樣課題沒研究協(xié)同作用什么的 ...

眼眶取血血液并非直接進(jìn)入收集的管子里，而是首先和眼角膜、眼瞼接觸后進(jìn)入。這就帶來一些額外未知的影響因素。在可能的情況下，能避免最好避免。提前給藥和同時給藥都屬于預(yù)防性給藥，即在未產(chǎn)生病理時即給予藥物干涉。這種應(yīng)用雖然重要，但是應(yīng)用面比較小。古語所說上醫(yī)治未病，下醫(yī)治已病。但是就經(jīng)濟(jì)和名氣而言，下醫(yī)最好。所以治療性給藥是研究的重點。后期處理應(yīng)該不會有太大改變，但是對于療效的機(jī)理解釋會有根本性變化。

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27樓2014-06-20 12:36:50

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27樓: Originally posted by richbird at 2014-06-20 12:36:50
眼眶取血血液并非直接進(jìn)入收集的管子里，而是首先和眼角膜、眼瞼接觸后進(jìn)入。這就帶來一些額外未知的影響因素。在可能的情況下，能避免最好避免。提前給藥和同時給藥都屬于預(yù)防性給藥，即在未產(chǎn)生病理時即給予藥物 ...

謝謝啦~~~初入代謝這一塊，有很多問題還很模糊，多謝！

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28樓2014-06-20 13:56:13

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最近也看到了這篇文獻(xiàn)，我覺得是不是因為采用肝門靜脈取血，首先可能就是后期實驗要做免疫組化，要求肝組織中無血，需要進(jìn)行肝灌流，也要進(jìn)行相關(guān)手術(shù)，涉及到肝門靜脈，所以在此就進(jìn)行肝門靜脈的手術(shù)，也節(jié)約的時間；其次因為取血量可能比眼眶取血大，利于滿足后期生化分析和代謝組學(xué)分析的血量要求。

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29樓2014-07-28 20:50:59

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