 |
 |
 |
| 24小時(shí)熱門版塊排行榜 |
 |
 |
 |
||
 |
 |
 |
 |
 |
| 5 | 1/1 | 返回列表 |
| 查看: 1005 | 回復(fù): 4 | ||
| 本帖產(chǎn)生 1 個(gè) 翻譯EPI ，點(diǎn)擊這里進(jìn)行查看 | ||
金獅水友至尊木蟲 (著名寫手)
|
[求助]
求英譯漢---生物傳感---177字80金幣-----一---翻譯好可以再加金幣
|
|
| MicroRNAs (total RNA) in cultured cancer cells were extracted using a RNA extraction kit from Qigen according to the recommended procedure, and miRNAs in the total RNA were enriched by using a cleanup kit (Qigen). Circulating miRNAs in serum were extracted using the miRNeasy RNA isolation kit from Qiagen. Briefly, 5 mL of Qiazol solution was added to 0.50 mL of serum. The mixture was thoroughly mixed and incubated for 10 min at room temperature. After 1.0 mL of chloroform was added, the mixture was vortexed for 60 s and centrifuged at 14 000 g for 15 min at 4 °C. Isolation and purification of miRNAs in the aqueous phase was achieved following the recommended procedure. Finally, miRNAs in the extracted total RNA samples were analyzed as described above. Reference values of miRNAs were obtained by qPCR (the gold standard). Direct profiling of circulating miRNAs in serum was conducted after spiking aliquots of concentrated TMD buffer to serum samples to have a final TMD buffer composition of 50 mM Tris-HCl, 5.0 mM MgCl2, and 1.0 mM DTT. |
回復(fù)此樓» 猜你喜歡
275求調(diào)劑
已經(jīng)有10人回復(fù)
-
286求調(diào)劑
已經(jīng)有6人回復(fù)
-
083000學(xué)碩274求調(diào)劑
已經(jīng)有5人回復(fù)
-
【求調(diào)劑】085601材料工程專碩 | 總分272 |
已經(jīng)有5人回復(fù)
-
275求調(diào)劑
已經(jīng)有13人回復(fù)
-
0856，材料與化工321分求調(diào)劑
已經(jīng)有8人回復(fù)
-
085701環(huán)境工程，267求調(diào)劑
已經(jīng)有12人回復(fù)
-
320分，材料與化工專業(yè)，求調(diào)劑
已經(jīng)有7人回復(fù)
-
085701環(huán)境工程求調(diào)劑
已經(jīng)有8人回復(fù)
-
295材料工程專碩求調(diào)劑
已經(jīng)有5人回復(fù)
2樓2013-05-15 16:19:08
金獅水友
至尊木蟲 (著名寫手)
- 應(yīng)助: 9 (幼兒園)
- 金幣: 12278.2
- 散金: 50
- 紅花: 13
- 沙發(fā): 5
- 帖子: 1686
- 在線: 203小時(shí)
- 蟲號(hào): 1817809
- 注冊(cè): 2012-05-15
- 性別: GG
- 專業(yè): 功能與智能高分子
呵呵，可以加金幣的哦 3樓2013-05-16 20:29:02
金獅水友
至尊木蟲 (著名寫手)
- 應(yīng)助: 9 (幼兒園)
- 金幣: 12278.2
- 散金: 50
- 紅花: 13
- 沙發(fā): 5
- 帖子: 1686
- 在線: 203小時(shí)
- 蟲號(hào): 1817809
- 注冊(cè): 2012-05-15
- 性別: GG
- 專業(yè): 功能與智能高分子
4樓2013-05-16 20:29:26
yili_90
金蟲 (正式寫手)
- 翻譯EPI: 17
- 應(yīng)助: 37 (小學(xué)生)
- 貴賓: 0.013
- 金幣: 3136.5
- 散金: 400
- 紅花: 11
- 帖子: 464
- 在線: 117.7小時(shí)
- 蟲號(hào): 2157293
- 注冊(cè): 2012-11-30
- 性別: MM
- 專業(yè): 動(dòng)物遺傳學(xué)
【答案】應(yīng)助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ...
金獅水友: 金幣+122, 翻譯EPI+1, ★★★★★最佳答案, 謝謝美女幫忙，3Q 2013-05-16 21:41:34
愛與雨下: 金幣+1 2013-05-16 21:46:59
金獅水友: 金幣+122, 翻譯EPI+1, ★★★★★最佳答案, 謝謝美女幫忙，3Q 2013-05-16 21:41:34
愛與雨下: 金幣+1 2013-05-16 21:46:59
| 內(nèi)容已刪除 |
5樓2013-05-16 21:34:52
相關(guān)版塊跳轉(zhuǎn)
我要訂閱樓主 金獅水友 的主題更新
| 5 | 1/1 | 返回列表 |
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 085701環(huán)境工程求調(diào)劑 +8 | 多久上課 2026-03-27 | 8/400 |
|
|---|---|---|---|---|
|
[考研] 295材料工程專碩求調(diào)劑 +5 | 1428151015 2026-03-27 | 5/250 |
|
|
|
[考研] 265求調(diào)劑 +8 | 小木蟲085600 2026-03-27 | 8/400 |
|
|
|
[考研] 086000調(diào)劑 +3 | 7901117076 2026-03-26 | 3/150 |
|
|
|
[考研] 求調(diào)劑 +4 | 零八# 2026-03-27 | 4/200 |
|
|
|
[考研] 266求調(diào)劑 +11 | 陽(yáng)陽(yáng)哇塞 2026-03-27 | 12/600 |
|
|
|
[考研] 化學(xué)調(diào)劑 +4 | 愛吃番茄的旭 2026-03-24 | 5/250 |
|
|
|
[考研] 0856調(diào)劑 +5 | 求求讓我有書讀?/a> 2026-03-26 | 6/300 |
|
|
|
[考研] 316求調(diào)劑 +5 | Pigcasso 2026-03-24 | 5/250 |
|
|
|
[考研] 一志愿211，335分，0856，求調(diào)劑院校和導(dǎo)師 +4 | 傾____蕭 2026-03-27 | 5/250 |
|
|
|
[考研] 0856材料化工調(diào)劑 總分330 +8 | zhubinhao 2026-03-27 | 8/400 |
|
|
|
[考研] 一志愿鄭大085600，310分求調(diào)劑 +5 | 李瀟可 2026-03-26 | 5/250 |
|
|
|
[考研] 304求調(diào)劑 +3 | 曼殊2266 2026-03-27 | 3/150 |
|
|
|
[考研] 342求調(diào)劑 +3 | 加油a李zs 2026-03-26 | 3/150 |
|
|
|
[考研] 324求調(diào)劑 +4 | wysyjs25 2026-03-21 | 4/200 |
|
|
|
[考研] 【雙一流院校新能源、環(huán)境材料，材料加工與模擬招收大量調(diào)劑】 +4 | Higraduate 2026-03-22 | 8/400 |
|
|
|
[考研]
 085602化學(xué)工程求調(diào)劑。
+4
|
平樂樂樂 2026-03-26 | 4/200 |
|
|
|
[考研] 機(jī)械學(xué)碩310分，數(shù)一英一，一志愿211本科雙非找調(diào)劑信息 +3 | @357 2026-03-25 | 3/150 |
|
|
|
[考研] 285求調(diào)劑 +3 | AZMK 2026-03-24 | 3/150 |
|
|
|
[考研] 求調(diào)劑 +5 | Zhangbod 2026-03-21 | 7/350 |
|