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| 本帖產(chǎn)生 1 個 翻譯EPI ，點擊這里進行查看 | ||
金獅水友至尊木蟲 (著名寫手)
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[求助]
求英譯漢---生物傳感---177字80金幣-----一---翻譯好可以再加金幣
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| MicroRNAs (total RNA) in cultured cancer cells were extracted using a RNA extraction kit from Qigen according to the recommended procedure, and miRNAs in the total RNA were enriched by using a cleanup kit (Qigen). Circulating miRNAs in serum were extracted using the miRNeasy RNA isolation kit from Qiagen. Briefly, 5 mL of Qiazol solution was added to 0.50 mL of serum. The mixture was thoroughly mixed and incubated for 10 min at room temperature. After 1.0 mL of chloroform was added, the mixture was vortexed for 60 s and centrifuged at 14 000 g for 15 min at 4 °C. Isolation and purification of miRNAs in the aqueous phase was achieved following the recommended procedure. Finally, miRNAs in the extracted total RNA samples were analyzed as described above. Reference values of miRNAs were obtained by qPCR (the gold standard). Direct profiling of circulating miRNAs in serum was conducted after spiking aliquots of concentrated TMD buffer to serum samples to have a final TMD buffer composition of 50 mM Tris-HCl, 5.0 mM MgCl2, and 1.0 mM DTT. |
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2樓2013-05-15 16:19:08
金獅水友
至尊木蟲 (著名寫手)
- 應(yīng)助: 9 (幼兒園)
- 金幣: 12278.2
- 散金: 50
- 紅花: 13
- 沙發(fā): 5
- 帖子: 1686
- 在線: 203小時
- 蟲號: 1817809
- 注冊: 2012-05-15
- 性別: GG
- 專業(yè): 功能與智能高分子
呵呵，可以加金幣的哦 3樓2013-05-16 20:29:02
金獅水友
至尊木蟲 (著名寫手)
- 應(yīng)助: 9 (幼兒園)
- 金幣: 12278.2
- 散金: 50
- 紅花: 13
- 沙發(fā): 5
- 帖子: 1686
- 在線: 203小時
- 蟲號: 1817809
- 注冊: 2012-05-15
- 性別: GG
- 專業(yè): 功能與智能高分子
4樓2013-05-16 20:29:26
yili_90
金蟲 (正式寫手)
- 翻譯EPI: 17
- 應(yīng)助: 37 (小學(xué)生)
- 貴賓: 0.013
- 金幣: 3136.5
- 散金: 400
- 紅花: 11
- 帖子: 464
- 在線: 117.7小時
- 蟲號: 2157293
- 注冊: 2012-11-30
- 性別: MM
- 專業(yè): 動物遺傳學(xué)
【答案】應(yīng)助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ...
金獅水友: 金幣+122, 翻譯EPI+1, ★★★★★最佳答案, 謝謝美女幫忙，3Q 2013-05-16 21:41:34
愛與雨下: 金幣+1 2013-05-16 21:46:59
金獅水友: 金幣+122, 翻譯EPI+1, ★★★★★最佳答案, 謝謝美女幫忙，3Q 2013-05-16 21:41:34
愛與雨下: 金幣+1 2013-05-16 21:46:59
| 內(nèi)容已刪除 |
5樓2013-05-16 21:34:52
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