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Atg3 has an/-fold, and its core region is topologically similar to canonical E2 enzymes. Atg3 has two regions inserted in the core region, one of which consists of80 residues and has a random coil structure in solution and another with a long -helical structure that protrudes from the core region as far as 30A˚ . In vivo and in vitro analyses suggested that the former region is responsible for binding Atg7, an E1-like enzyme, and that the latter is responsible for binding Atg8.Asulfate ion was bound near the catalytic cysteine of Atg3, suggesting a possible binding site for the phosphate moiety of PE. |
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| ATG3有一α/β折疊,其核心域在拓?fù)渖项愃朴诘湫偷腅2酶。ATG3有兩個(gè)區(qū)域插入核心域,其中一個(gè)由約80個(gè)殘基組成,在溶液中具有無(wú)規(guī)卷曲結(jié)構(gòu);另一個(gè)則具有長(zhǎng)α-螺旋結(jié)構(gòu),自核心域突出達(dá)30 Å。體內(nèi)和體外分析表明,前一個(gè)區(qū)域負(fù)責(zé)結(jié)合ATG7,ATG7是一種E1樣酶;而后一區(qū)域則負(fù)責(zé)結(jié)合ATG8。ATG3的催化半胱氨酸附近結(jié)合一硫酸根離子,提示可能是磷脂(PE)磷酸基團(tuán)的結(jié)合位點(diǎn)。 |
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