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Atg3 has an/-fold, and its core region is topologically similar to canonical E2 enzymes. Atg3 has two regions inserted in the core region, one of which consists of80 residues and has a random coil structure in solution and another with a long -helical structure that protrudes from the core region as far as 30A˚ . In vivo and in vitro analyses suggested that the former region is responsible for binding Atg7, an E1-like enzyme, and that the latter is responsible for binding Atg8.Asulfate ion was bound near the catalytic cysteine of Atg3, suggesting a possible binding site for the phosphate moiety of PE. |
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| ATG3有一α/β折疊,其核心域在拓撲上類似于典型的E2酶。ATG3有兩個區(qū)域插入核心域,其中一個由約80個殘基組成,在溶液中具有無規(guī)卷曲結構;另一個則具有長α-螺旋結構,自核心域突出達30 Å。體內和體外分析表明,前一個區(qū)域負責結合ATG7,ATG7是一種E1樣酶;而后一區(qū)域則負責結合ATG8。ATG3的催化半胱氨酸附近結合一硫酸根離子,提示可能是磷脂(PE)磷酸基團的結合位點。 |
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