提交序列至NCBI后,說我序列質(zhì)量低
請(qǐng)教各位大神,我向NCBI提交序列,出現(xiàn)了問題,首先說一下我的這個(gè)序列是經(jīng)過測(cè)序驗(yàn)證過的,但是為什么說我序列質(zhì)量低呢?
請(qǐng)大家看看發(fā)過來的郵件,幫幫孩子吧!
We noticed that regions of your sequence(s) include numerous ambiguous bases or stretches of the same nucleotide base. It therefore appears that the sequences in these regions are of low quality, and we are unable to accept your sequences in their current format.
We welcome you to resubmit after the low quality regions have been trimmed or resequenced to provide better quality data. Alternatively, you can remove the low quality sequences entirely and resubmit the revised file. If you trim, the remaining sequences must be at least 200 bases long.
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