提交序列至NCBI后，說我序列質(zhì)量低
請教各位大神，我向NCBI提交序列，出現(xiàn)了問題，首先說一下我的這個序列是經(jīng)過測序驗證過的，但是為什么說我序列質(zhì)量低呢？
請大家看看發(fā)過來的郵件，幫幫孩子吧！
We noticed that regions of your sequence(s) include numerous ambiguous bases or stretches of the same nucleotide base. It therefore appears that the sequences in these regions are of low quality, and we are unable to accept your sequences in their current format.
We welcome you to resubmit after the low quality regions have been trimmed or resequenced to provide better quality data. Alternatively, you can remove the low quality sequences entirely and resubmit the revised file. If you trim, the remaining sequences must be at least 200 bases long.
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你提交的是什么序列啊 測序文件嗎？
你需要看一下測序的峰圖，看有沒有重疊峰�？梢栽诎俣容斎搿澳芫幋a蛋白質(zhì)的基因序列提交NCBI”就會有提交教程。希望能幫到你
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