 |
 |
 |
 |
 |
 |
||
 |
 |
 |
 |
 |
| 1 | 1/1 | 返回列表 |
| 查看: 455 | 回復(fù): 0 | ||
suchanghui木蟲 (小有名氣)
|
[求助]
求助藥學(xué)實驗過程翻譯！寫文章用，一定要專業(yè)！
|
|
Hydrolysis The stock solutions of complexes 1–5 (2 mM) were prepared in methanol. Then 5 mL of the solution (10 mL for complex 2) was diluted to 200 mL with deionized water in a quartz cuvette and the UV-vis spectra was recorded by scanning from 200 to 500 nm at certain time intervals at 37 1C. The wavelengths corresponding to the LMCT band at ca. 330–336 nm were selected for the kinetic study. The time-dependent absorbance were fitted using Origin 8.0 (OriginLab Corporation, US) to give the first order rate constant k,and half reaction time t1/2was calculated by the formula as follows: A = Ce-kt + A0；t1/2 = ln2/k； where A is the absorbance, and A0 and C are constants. The species before and after hydrolysis for 1 h were analysed by LC-MS as described below. High performance liquid chromatography (HPLC) A Rheodyne sample injector, an Agilent Eclipse XDB-C18 reversed phase column (150×4.6 mm, 5um, USA) and an Agilent 1200 series HPLC system were used to analyse the hydrolytic mixtures of complexes 1–5. The mobile phases were water containing 0.1% TFA (A), and acetonitrile containing 0.1% TFA (B). The gradient was increased from 10% to 80% B for 20 min, with a flow rate of 1 mL min-1. DNA interaction Hoechst 33342 (20 uM) and CT DNA (200 uM) were dissolved in Tris-HCl buffer (pH = 7.2) and incubated at ambient temperature for 4 h. Then complex 1 or 3 (0–16 mM) was added to this solution to make the final concentrations from 0 to 160 uM. After incubating for 12 h, the resulting mixture was measured on an F-4500 fluorescence spectrophotometer (HITACHI) with excitation wavelength at 370 nm and emission spectra from 400 to 650 nm. A modified Stern–Volmer plot was employed to evaluate the affinity of complexes towards DNA. Different F0/F values recorded at 490 nm with different concentrations of complex 1 or 3 was fitted using Origin 8.0 (OriginLab Corporation, US) and Ksv was calculated by equations as follows: F0/F = 1 + Ksv[Q] where F0 and F are the fluorescence intensities of the Hoechst–CT DNA complex recorded before and after adding complex 1 or 3, respectively. [Q] is the concentration of complex 1 or 3. |
回復(fù)此樓» 猜你喜歡
327求調(diào)劑
已經(jīng)有4人回復(fù)
-
085600材料與化工調(diào)劑
已經(jīng)有3人回復(fù)
-
309求調(diào)劑
已經(jīng)有8人回復(fù)
-
一志愿南航 335分 | 0856 | GPA 4.07 | 有科研經(jīng)歷
已經(jīng)有8人回復(fù)
-
求調(diào)劑
已經(jīng)有5人回復(fù)
-
求化學(xué)調(diào)劑
已經(jīng)有5人回復(fù)
-
085600，材料與化工321分求調(diào)劑
已經(jīng)有10人回復(fù)
-
0703 化學(xué) 求調(diào)劑，一志愿山東大學(xué) 342 分
已經(jīng)有5人回復(fù)
-
一志愿南開大學(xué)0710生物學(xué)359求調(diào)劑
已經(jīng)有3人回復(fù)
-
085600，專業(yè)課化工原理，320分求調(diào)劑
已經(jīng)有4人回復(fù)
相關(guān)版塊跳轉(zhuǎn)
我要訂閱樓主 suchanghui 的主題更新
| 1 | 1/1 | 返回列表 |
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 290求調(diào)劑 +3 | dfffsar 2026-03-29 | 3/150 |
|
|---|---|---|---|---|
|
[考研] 277跪求調(diào)劑 +6 | 1915668 2026-03-27 | 10/500 |
|
|
|
[考研] 297求調(diào)劑 +11 | 田洪有 2026-03-26 | 11/550 |
|
|
|
[考研] 求調(diào)劑一志愿武漢理工大學(xué)材料工程（085601） +7 | WW.' 2026-03-23 | 9/450 |
|
|
|
[考研] 375求調(diào)劑 +4 | 雨夏整夜 2026-03-29 | 4/200 |
|
|
|
[考研] 356求調(diào)劑 +4 | gysy?s?a 2026-03-28 | 4/200 |
|
|
|
[考研] 289求調(diào)劑 +13 | 新時代材料 2026-03-27 | 13/650 |
|
|
|
[考研] 356求調(diào)劑 +3 | gysy?s?a 2026-03-28 | 3/150 |
|
|
|
[考研] 藥學(xué)105500求調(diào)劑 +3 | Ssun。。 2026-03-28 | 3/150 |
|
|
|
[考研] 311求調(diào)劑 +3 | 希望上岸阿小楊 2026-03-23 | 3/150 |
|
|
|
[考研] 安徽大學(xué)專碩生物與醫(yī)藥專業(yè)(086000)324分，英語已過四六級，六級521，求調(diào)劑 +4 | 美味可樂雞翅 2026-03-26 | 4/200 |
|
|
|
[考研] 322求調(diào)劑 +4 | 我真的很想學(xué)習(xí) 2026-03-23 | 4/200 |
|
|
|
[考研] 336材料求調(diào)劑 +7 | 陳瀅瑩 2026-03-26 | 9/450 |
|
|
|
[考研] 303求調(diào)劑 +6 | 藍山月 2026-03-25 | 6/300 |
|
|
|
[考研] 材料專碩 335 分求調(diào)劑 +4 | 拒絕冷暴力 2026-03-25 | 4/200 |
|
|
|
[考研] 考研一志愿蘇州大學(xué)初始315（英一）求調(diào)劑 +3 | sbdksD 2026-03-24 | 4/200 |
|
|
|
[考研] 0854人工智能方向招收調(diào)劑 +4 | 章小魚567 2026-03-24 | 4/200 |
|
|
|
[考研] 292求調(diào)劑 +4 | 鵝鵝鵝額額額額?/a> 2026-03-24 | 4/200 |
|
|
|
[考研] 一志愿國科過程所081700，274求調(diào)劑 +3 | 三水研0水立方 2026-03-23 | 3/150 |
|
|
|
[考研] 336化工調(diào)劑 +4 | 王大坦1 2026-03-23 | 5/250 |
|