 |
 |
 |
 |
 |
 |
||
 |
 |
 |
 |
 |
| 5 | 1/1 | 返回列表 |
| 查看: 683 | 回復(fù): 2 | ||
| 當(dāng)前只顯示滿足指定條件的回帖，點(diǎn)擊這里查看本話題的所有回帖 | ||
1570583814新蟲 (初入文壇)
|
[求助]
求蛋白質(zhì)提取方法的簡潔順暢英文概括，具體步驟如下 已有1人參與
|
|
|
1) The sample was grinded to power with liquid nitrogen; 2) Add 10mL cooled acetone contained 10% TCA to 1g sample power at -20°C for 1 hour; 3) Centrifuge by 15000g for 15 min at 4°C, collect the deposit and add cooled acetone at -20°C for 1 hour; 4) Repeated the step 3; 5) Centrifuge by 15000g for 15 min at 4°C, collect the deposit and dried by vacuum freezed dryer; 6) Suspends the deposit in cold phenol extraction buffer; 7) Add an equal volume of phenol saturated with Tris-HCl (pH 7.5) and shake the mixture for 30 min at 4 ℃; 8) Centrifuge at 5000g for 30 min at 4 ℃ and collect the upper phenolic phase; 9) Add equal phenol extraction buffer to the collected phenolic phase, repeat steps 2-3 and collect the upper phenolic phase again; 10) Add 5 volumes of cold 0.1 M ammonium acetate in methanol to the collected phenol phase, store at –20 ℃ for 1 hour; 11) Centrifuge the sample for 30 min at 5000g at 4 ℃; 12) Add 2 volumes (based on the volume of the last collected phenolic phase) ice-cold methanol to wash the pellet, mix gently; 13) Centrifuge the sample for 30 min at 5000g at 4 ℃; 14) Repeat Steps 12and 13 two more times; 15) Repeat Steps 12 and 13 two more times using acetone instead of methanol; 16) Centrifuge the sample for 30 min at 5000g at 4 ℃, dry the deposit; 17) The deposit was dissolved in lysis solution at 30°C for 1 hour; 18) Centrifuge the solution by 15000g for 15 min at room temperature, collect the supernatant and centrifuge again; 19) The supernatant was the extracted protein solution. 發(fā)自小木蟲IOS客戶端 |
回復(fù)此樓» 猜你喜歡
材料調(diào)劑
已經(jīng)有3人回復(fù)
-
考研調(diào)劑
已經(jīng)有5人回復(fù)
-
26申博
已經(jīng)有4人回復(fù)
-
調(diào)劑申請
已經(jīng)有7人回復(fù)
-
環(huán)境工程297分求調(diào)劑一志愿杭高院
已經(jīng)有8人回復(fù)
-
070300化學(xué)調(diào)劑
已經(jīng)有7人回復(fù)
-
一志愿085600中科院寧波所276分求調(diào)劑
已經(jīng)有15人回復(fù)
-
288資源與環(huán)境專碩求調(diào)劑，不限專業(yè)，有學(xué)上就行
已經(jīng)有22人回復(fù)
-
273求調(diào)劑
已經(jīng)有8人回復(fù)
-
267求調(diào)劑
已經(jīng)有9人回復(fù)
1570583814
新蟲 (初入文壇)
- 應(yīng)助: 0 (幼兒園)
- 金幣: 315.9
- 散金: 70
- 帖子: 40
- 在線: 14.9小時(shí)
- 蟲號: 4170639
- 注冊: 2015-10-24
- 性別: MM
- 專業(yè): 作物種質(zhì)資源與遺傳育種學(xué)
3樓2016-03-09 13:10:39
★ ★
感謝參與，應(yīng)助指數(shù) +1
空位: 金幣+2, 金幣獎勵(lì)，辛苦了o(∩_∩)o 2016-03-18 14:15:08
感謝參與，應(yīng)助指數(shù) +1
空位: 金幣+2, 金幣獎勵(lì)，辛苦了o(∩_∩)o 2016-03-18 14:15:08
|
本帖內(nèi)容被屏蔽 |
2樓2016-03-09 10:23:45
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 環(huán)境工程297分求調(diào)劑一志愿杭高院 +8 | GENJIOW 2026-03-31 | 8/400 |
|
|---|---|---|---|---|
|
|
[電化學(xué)] 070300化學(xué)調(diào)劑 +7 | 山頂見α 2026-03-25 | 7/350 |
|
|
[論文投稿]
chinese chemical letters英文版投稿求助
 130+4
|
Yishengeryi 2026-03-30 | 4/200 |
|
|
|
[考研] 求調(diào)劑：085600材料與化工，考材科基，總分319 +16 | 678lucky 2026-03-31 | 20/1000 |
|
|
|
[考研] 299求調(diào)劑 +8 | 嗯嗯嗯嗯2 2026-03-27 | 8/400 |
|
|
|
[考研] 本科211安全工程，初試290分，求調(diào)劑 +3 | 2719846834 2026-03-28 | 3/150 |
|
|
|
[考研] 一志愿西電085401數(shù)一英一299求調(diào)劑 六級521 +4 | 愛吃大鴨梨 2026-03-31 | 4/200 |
|
|
|
[考研] 085601材料工程找調(diào)劑 +17 | oatmealR 2026-03-29 | 18/900 |
|
|
|
[考研] 332求調(diào)劑 +6 | @MZB382400 2026-03-28 | 6/300 |
|
|
|
[考研] 332求調(diào)劑 +6 | Lyy930824@ 2026-03-29 | 6/300 |
|
|
|
[考研] 求調(diào)劑 +10 | 張zz111 2026-03-27 | 11/550 |
|
|
|
[考研] 085600，材料與化工321分求調(diào)劑 +10 | 大饞小子 2026-03-28 | 10/500 |
|
|
|
[考研] 349求調(diào)劑 +6 | 李木子啊哈哈 2026-03-25 | 6/300 |
|
|
|
[考研] 340求調(diào)劑 +6 | Amber00 2026-03-26 | 6/300 |
|
|
|
[考研] 305求調(diào)劑 +8 | RuiFairyrui 2026-03-28 | 8/400 |
|
|
|
[考研] 壓國家一區(qū)線，求導(dǎo)師收留，有恩必謝！ +7 | 迷人的哈哈 2026-03-28 | 7/350 |
|
|
|
[考研] 0703化學(xué)求調(diào)劑，各位老師看看我�。�！ +5 | 祁祺祺 2026-03-25 | 5/250 |
|
|
|
[考研] 348求調(diào)劑 +4 | 小懶蟲不懶了 2026-03-27 | 5/250 |
|
|
|
[考研] 324求調(diào)劑 +5 | hanamiko 2026-03-26 | 5/250 |
|
|
|
[考研] 286求調(diào)劑 +4 | lim0922 2026-03-26 | 4/200 |
|