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[求助]
注射器發(fā)酵裝置
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最近在文獻(xiàn)中看到了一種簡(jiǎn)易的BMP測(cè)定方法，利用100mL注射器作為發(fā)酵裝置，想求教使用過(guò)這種方法的前輩一些問(wèn)題： 我看的文獻(xiàn)中提到了使了100mL注射器、30mL基質(zhì)、頂部三通閥、硅膠密封、恒溫振蕩器， 那么在實(shí)際進(jìn)行中有什么需要注意的地方么（包括實(shí)驗(yàn)過(guò)程以及發(fā)酵裝置的細(xì)節(jié)）？ 對(duì)于注射器有什么要求么？ 對(duì)于基質(zhì)（主要是基質(zhì)的產(chǎn)期能力）有沒(méi)有限制？ 謝謝大家！  下面是文獻(xiàn)中的方法，引用的參考文獻(xiàn)都是書(shū)、下不到 The anaerobic digestion tests were conducted based on the well-established biochemical methane potential (BMP) procedure, as described in the guideline VDI 4630 (VDI, 2006). In detail, the batch fermentations were carried out in 100 mL glass syringes according to the Hohenheimer Biogas Yield Test (HBT) (Helffrich and Oechsner, 2003). To ensure anaerobic conditions and to collect the biogas, the syringes were gas tightened by using mediumviscosity silicone paste (GE Bayer Silicones GmbH & Co KG, Erkrath, Germany). Three way valves allowed sampling of the biogas. For stirring and heating, the syringes were placed in a continuously rotating vertical disc (5 rpm) located in a 42 C heating chamber. VDI, 2006. Fermentation of Organic Materials – Characterisation of the Substrate, Sampling, Collection of Material Data, Fermentation Tests. Beuth Verlag, Berlin. Helffrich, D., Oechsner, H., 2003. Hohenheimer biogas yield test – comparing various laboratory methods on biomass fermentation. Landtechnik 58, 148– 149. |
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