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[求助]
纖維素酶測(cè)量方法
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在測(cè)量纖維素酶的過程中,文獻(xiàn)介紹”O(jiān)ne unit of activity is defined as the amount of the enzyme required to release 1 umol reducing groups per min per g of original fresh weight of berry flesh.“ 但是文獻(xiàn)并沒有介紹具體公式,請(qǐng)問這個(gè)計(jì)算公式是什么,以及”D-glucose as the standard“是不是需要標(biāo)準(zhǔn)曲線的意思?希望各位大神能幫忙解答下 原文如下: “Cellulase activity was determined by measuring the reducing groups released from carboxymethyl cellulose (Chin, Ali, & Lazan, 1999). The formation of reducing groups was estimated against D-glucose as the standard. The reaction mixture contained 0.1 ml of crude enzyme, 0.4 ml of 1% (w/v) carboxymethyl cellulose, and 0.5 ml 0.1 M sodium acetate buffer, pH 5.0. Incubation was carried out at 37 C for 1 h followed by addition of DNS. In control tubes the substrate was added after the heat treatment. After heating in a boiling water bath for 5 min, the absorbance was measured at 540 nm. One unit of activity is defined as the amount of the enzyme required to release 1 umol reducing groups per min per g of original fresh weight of berry flesh.“ |
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