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| The bidirectional promoter site between the rebG and rebO genes was replaced with a TRP1 bidirectional promoter cassette and the unidirectional promoter in the upstream region of the rebR gene was replaced with a MET15 cassette in which only one promoter was incorporated into the amplicon used for recombination (i.e., aunidirectional promoter cassette) (SI Appendix). |
至尊木蟲 (著名寫手)
| 以TRP1雙向啟動(dòng)子盒替換rebG 和 rebO基因之間的雙向啟動(dòng)子位點(diǎn),并以MET15盒替換rebR基因上游區(qū)域中的單向啟動(dòng)子。在MET15盒中只有一個(gè)啟動(dòng)子引入用于重組的擴(kuò)增子中(如,一個(gè)單向啟動(dòng)子盒)(附錄SI) |
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