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The outer edges of the Lzr gene cluster were defined based on comparisons to the BE-54017 and cladoniamide gene clusters and a BLAST analysis of genes surrounding the core indolotryptoline biosynthesis genes (Fig. 3A). The biosynthesis of indolotryptolines is well-characterized, making it possible to predict the function of most genes in the Lzr gene cluster (32). The four key stages of indolotryptoline biosynthesis involve dimerization of oxo-tryptophan to form a chromopyrrolic acid, oxidative aryl–aryl coupling to form an indolocarbazole, “flipping” of one of the indole rings to form an indolotryptoline, and tailoring to generate the final product. The Lzr gene cluster is predicted to contain seven transcriptional units controlled by three bidirectional (P1, P2, and P3) and one unidirectional (P4) promoter regions (Fig. 3 B, i). This cluster is conveniently organized such that successive activation of the three bidirectional promoter regions (P1, P2, and P3) is predicted to drive the expression of genes required to achieve the first, second, and third stages in indolotryptoline biosynthesis, respectively (Fig. 3C). |
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3樓2016-03-28 21:44:30
wangqiao12
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2樓2016-03-28 00:35:06
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4樓2016-03-28 21:44:54
818james
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【答案】應(yīng)助回帖
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bxzc123(fjtony163代發(fā)): 金幣+30, 代發(fā) 2016-04-29 14:14:22
bxzc123(fjtony163代發(fā)): 金幣+30, 代發(fā) 2016-04-29 14:14:30
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bxzc123(fjtony163代發(fā)): 金幣+30, 代發(fā) 2016-04-29 14:14:22
bxzc123(fjtony163代發(fā)): 金幣+30, 代發(fā) 2016-04-29 14:14:30
bxzc123(fjtony163代發(fā)): 金幣+30, 代發(fā) 2016-04-29 14:14:37
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fjtony163: 翻譯EPI+1, 代發(fā) 2016-04-29 14:14:50
|
The outer edges of the Lzr gene cluster were defined based on comparisons to the BE-54017 and cladoniamide gene clusters and a BLAST analysis of genes surrounding the core indolotryptoline biosynthesis genes (Fig. 3A). The biosynthesis of indolotryptolines is well-characterized, making it possible to predict the function of most genes in the Lzr gene cluster (32). The four key stages of indolotryptoline biosynthesis involve dimerization of oxo-tryptophan to form a chromopyrrolic acid, oxidative aryl–aryl coupling to form an indolocarbazole, “flipping” of one of the indole rings to form an indolotryptoline, and tailoring to generate the final product. The Lzr gene cluster is predicted to contain seven transcriptional units controlled by three bidirectional (P1, P2, and P3) and one unidirectional (P4) promoter regions (Fig. 3 B, i). This cluster is conveniently organized such that successive activation of the three bidirectional promoter regions (P1, P2, and P3) is predicted to drive the expression of genes required to achieve the first, second, and third stages in indolotryptoline biosynthesis, respectively (Fig. 3C). 通過與BE-54017 和 cladoniamide 基因簇比較以及對(duì) indolotryptoline生物合成核心基因的外圍基因進(jìn)行比對(duì)分析(Fig. 3A)來確定 Lzr 基因簇的外部邊界。Indolotryptolines的生物合成特點(diǎn)突出，根據(jù)這些特點(diǎn)可預(yù)測lzr基因簇中很多基因的功能。Indolotryptolines生物合成的四個(gè)主要的步驟包括氧絡(luò)色氨酸的二聚化形成chromopyrrolic acid，氧化芳基連接成indolocarbazole，一個(gè)吲哚環(huán)翻轉(zhuǎn)形成indolotryptoline，修飾形成最終產(chǎn)物。預(yù)測lzr基因簇含有七個(gè)轉(zhuǎn)錄單位，它們由三個(gè)雙向(P1, P2, 和 P3)，一個(gè)單向(P4)的啟動(dòng)子區(qū)域控制(Fig. 3 B, i)。該基因簇的排列使人很易想到三個(gè)雙向啟動(dòng)子區(qū)域(P1, P2, 和 P3)的相繼啟動(dòng)是分別促使indolotryptoline生物合成的第一步，第二步，第三步反應(yīng)所需的基因的表達(dá)。 |
5樓2016-04-01 11:58:26
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