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讓她降落銅蟲 (正式寫手)
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[求助]
求一段關(guān)于細(xì)菌胞內(nèi)DNA以及總DNA提取的完整翻譯
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| In particular, DNA was extracted from bacterial colonies picked up from plates, and resuspended in 1.0mL of sterile water up to obtain 10的9次方 harvest cells useful for DNA extraction. Cellular density of water bacterial suspensions was spectroscopically measured (three replicates) by optical density at 600nm. TotalDNA (e.g., genetic material of live bacteria and/or released from inactivated bacteria) was extracted from UV/H2O2 treated watersamples collected at each time. Aliquots (1.0 mL) of each water sample were centrifuged for 10min at 14,000rpm in order to collect and concentrate genetic material up 0.25 OD (600nm). Pellets were resuspended in 180.0 μL of extraction buffer (ATL) and used for DNA extraction. |
至尊木蟲 (知名作家)
Translator and Proofreader
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這個(gè)我最熟悉,就給你說(shuō)說(shuō)吧(不一定嚴(yán)格按字面翻譯): 具體地說(shuō),DNA是從培養(yǎng)板挑取的細(xì)菌菌落中提取的:提取菌落并懸浮于1.0mL無(wú)菌水中,得到10的9次方細(xì)菌,用于DNA提取。水中細(xì)菌懸浮液的細(xì)胞密度用分光光度計(jì)于600 nm 讀取OD值(即OD600值,重復(fù)三次)。 用 UV/H2O2 處理水樣品,不同時(shí)間后收集水樣,提取總DNA(從活細(xì)菌和/或滅活細(xì)菌釋放的遺傳物質(zhì))。每個(gè)水樣本的等分試樣(1.0mL)以14,000 rpm離心10分鐘,以便收集并濃縮遺傳物質(zhì),使OD600的讀數(shù)高達(dá)0.25。離心沉渣懸浮于180.0微升提取緩沖液(ATL)用于DNA提取。 |
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