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lnlnln木蟲 (正式寫手)
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[求助]
冷凍干燥機(jī)使用 已有1人參與
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| 由于凍干樣品特別少(5mL的瓶,樣品是200uL),在冷凍干燥時(shí)溫度探頭該怎么放置好呢?才能精確地監(jiān)測物料溫度? |
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No need sensor such small sample, you set temperature at what you want, say 4C the sample can be frozen in advance before dry. More sample to freeze dry to set temperature and switch vacuum few house or overnight sample will be ready and temperature will never over 4C Sample 200ul, not sure how much solid residue. you may add bulk agent for example sugar etc |
木蟲 (正式寫手)
木蟲 (正式寫手)
木蟲 (正式寫手)
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sugar is not good, easy boiling, there are lots of other carrier can use, depends on which active compounds in dry. If you cannot set temperature, you have to treat the shelf temperature as room temperature If you provide more information about your active compound, I may provide more advice. |
木蟲 (正式寫手)
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The sample belongs to a kind of antigenic proteins, into which sucrose should be added to protect its activity in process of freeze-drying. Whether we could put ice or ice bag onto the shelf has been assayed and we found the shape after freeze-drying was awful just like sugar boiling. 發(fā)自小木蟲Android客戶端 |
木蟲 (正式寫手)
木蟲 (正式寫手)
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