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運用多級注射的方法對動力學(xué)參數(shù)進行表征
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ABSTRACT Reactions, including enzymatic reactions, produce or absorb heat. This heat can be directly related to the product formation in an enzymatic reaction. The advantage to using heat as a metric is that the materials don’t require modification, such as the addition of a chromophore, and the assay is continuous without the need to quench the reaction at various points for product quantification. One of the only requirements is that the substrate and enzyme buffers are matched, which is ideally accomplished through dialysis of the enzyme and substrate in the same buffer. |
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歡迎監(jiān)督和反饋：小木蟲僅提供交流平臺，不對該內(nèi)容負(fù)責(zé)。
本內(nèi)容由用戶自主發(fā)布，如果其內(nèi)容涉及到知識產(chǎn)權(quán)問題，其責(zé)任在于用戶本人，如對版權(quán)有異議，請聯(lián)系郵箱：xiaomuchong@tal.com - 附件 1 : 采用等溫滴定量熱儀（ITC）運用多級注射的方法對于動力學(xué)的參數(shù)進行表征實驗.pdf
2016-07-05 09:40:28, 206.79 K
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