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[求助]
一般怎么找到這個(gè)Tub-tag的啊
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Abstract: A novel chemoenzymatic approach for simple and fast site-specific protein labeling is reported. Recombinant tubulin tyrosine ligase (TTL) was repurposed to attach various unnatural tyrosine derivatives as small bioorthogonal handles to proteins containing a short tubulin-derived recognition sequence (Tub-tag). This novel strategy enables a broad range of high-yielding and fast chemoselective C-terminal protein modifications on isolated proteins or in cell lysates for applications in biochemistry, cell biology, and beyond, as demonstrated by the site-specific labeling of nanobodies, GFP,and ubiquitin. 這是一個(gè)論文的摘要,作者在文章提到TTL酶可以特異識別一個(gè)14個(gè)氨基酸組成的多肽,也就是這個(gè)Tub-tag。我想了解的是:一般怎么找到這個(gè)Tub-tag的啊,用什么方法啊。 1.how can you find the fourteen amino sequence, which can be served as tubulin-derived recognition sequence (Tub-tag)? 2. what methods will you need to find the sequence? 3.or what is the general process to find the sequence? 求大神解答。 萬分感激! |

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