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永布拉格之春新蟲 (初入文壇)
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[求助]
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為什么內(nèi)切酶識別回文序列？究竟什么是回文序列？那位跟我解釋一下？ @biostar2009 發(fā)自小木蟲Android客戶端 |
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永布拉格之春
新蟲 (初入文壇)
- 應(yīng)助: 0 (幼兒園)
- 金幣: 74.1
- 散金: 45
- 帖子: 26
- 在線: 17.7小時(shí)
- 蟲號: 4491266
- 注冊: 2016-03-11
- 專業(yè): 生物化學(xué)
2樓2016-11-05 11:55:57
【答案】應(yīng)助回帖
感謝參與，應(yīng)助指數(shù) +1
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The sequence of enzyme digestion site usually a sequence in which the same sequence shows in both direction strands. Or restriction site. Restriction sites, or restriction recognition sites, are locations on a DNA molecule containing specific (4-8 base pairs in length) sequences of nucleotides, which are recognized by restriction enzymes. These are generally palindromic sequences (because restriction enzymes usually bind as homodimers), and a particular restriction enzyme may cut the sequence between two nucleotides within its recognition site, or somewhere nearby. For example, the common restriction enzyme EcoRI recognizes the palindromic sequence GAATTC and cuts between the G and the A on both the top and bottom strands, leaving an overhang (an end-portion of a DNA strand with no attached complement) known as a sticky end on each end of AATT. This overhang can then be used to ligate in (see DNA ligase) a piece of DNA with a complementary overhang (another EcoRI-cut piece, for example). Some restriction enzymes cut DNA at a restriction site in a manner which leaves no overhang, called a blunt end. |
3樓2016-11-05 12:47:49
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