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詩意花香銅蟲 (初入文壇)
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[求助]
變異鏈球菌Ingbritt株 已有2人參與
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變異鏈球菌Ingbritt株的公認(rèn)編號有沒有呢？比如ATCC、CCTCC。它的菌種來源是哪里呢？臨床分離？如果是國際標(biāo)準(zhǔn)菌株，為什么沒有標(biāo)準(zhǔn)編號呢？它跟UA159有什么關(guān)系？準(zhǔn)備做蛋白組學(xué)，但在uniprot 數(shù)據(jù)庫里查不到這個變異鏈球菌的這種菌株，也就找不到標(biāo)準(zhǔn)菌株蛋白數(shù)據(jù)庫，只查的到UA159的。 @天使托 發(fā)自小木蟲IOS客戶端 |
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2樓2016-11-15 17:50:40
jql81070305
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【答案】應(yīng)助回帖
★ ★ ★ ★ ★
感謝參與，應(yīng)助指數(shù) +1
詩意花香: 金幣+5 2016-11-17 10:28:41
感謝參與，應(yīng)助指數(shù) +1
詩意花香: 金幣+5 2016-11-17 10:28:41
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這是伯杰氏手冊里介紹變異鏈球菌那節(jié)~ 32. Streptococcus mutans Clarke 1924, 144AL mu′tans. L. part. adj. mutans changing. Cells are coccoid, approximately 0.5–0.75 μm in diameter,occurring in pairs or as short- to medium-length chains without capsules. In acid conditions in broth and on some solid media, Streptococcus mutans may form short rods (1.5–3.0 μm in length) and rod-shaped morphology may be evident on primary isolation from oral specimens.Growth on blood agar after incubation anaerobically for 2 d produces colonies that are white or gray, circular or irregular, 0.5–1.0 mm in diameter, sometimes rather hard and tending to adhere to the surface of the agar and slightly pitting into the agar surface. Hemolytic reaction on blood agar is usually α-hemolytic or non-hemolytic with very occasionally strains giving β-hemolysis. Growth on sucrose-containing agar typically produces rough, heaped colonies, about 1 mm in diameter with soluble extracellular polysaccharide visible as beads, droplets, or puddles of liquid on or surrounding the colonies. Some strains may form smooth or mucoid colonies (Edwardsson, 1968,1970). Facultatively anaerobic; while most strains grow in air growth is optimum at 37°C under anaerobic condition with some strains CO2-dependent. A few strains have been reported to grow at 45°C, but no growth occurs at 10°C. Acid is produced from arbutin, d-cellobiose, esculin, galactose, glucose, inulin, lactose, maltose, mannitol, mannose, N-acetylglucosamine, salicin, sorbitol, sucrose, trehalose, and d-raffinose. Melibiose and amygdalin fermentation are variable characteristics of Streptococcus mutans with strainsthat cannot ferment melibiose being less frequently able to produce acid from raffinose or amygdalin. Acid is notproduced from adonitol, arabinose, cyclodextrin, dulcitol,erythritol, glycerol, glycogen, gluconate, inositol, methyl d-glucoside, methyl d-mannoside, methyl d-xyloside, melezitose, rhamnose, ribose, sorbose, starch, or xylose.Strains hydrolyze esculin but not arginine. Starch is hydrolyzed by the majority of strains but hippurate and urea are not. Strains produce leucine aminopeptidase, valine aminopeptidase, chymotrypsin, phosphoamidase, α- and β-glucosidase, α-galactosidase, and β-lactosidase. Strains do not produce alkaline phosphatase, β-glucosaminidase, glycyl tryptophan arylamidase, N-acetyl-β-d-galactosaminidase, N-acetyl-β-d-glucosaminidase, β-d-glucuronidase, α-lfucosidase, β-d-fucosidase, β-mannosidase, β-maltosidase, α-arabinosidase, pyrrolidonyl arylamidase, urease, hyaluronidase, orneuraminidase (sialidase). α-maltosidase is a variable characteristic of this species. Melibiose nonfermenting strains do not produce α-galactosidase or α-glucosidase. Test results for β-d-galactosidase production have been shown to vary depending on the substrate derivative employed (Kilian et al., 1989b). Acetoin is produced by the majority of strains. Hydrogen peroxide is not produced.Strains are non-groupable with Lancefield grouping antisera. Strains contain the cell-wall polysaccharide-typing antigens c, e, or f, according to the serological scheme of Bratthall (1970) and Perch et al. (1974). Extracellular polysaccharide (dextran) is produced from sucrose. The majority of strains are resistant to bacitracin. Strains are isolated from the oral cavity where the primary habitat is the tooth surface (dental plaque) and colonization is favored by high levels of dietary sucrose. Strains have been isolated from feces. 16S rRNA gene sequence analysis places this species within the Mutans species group. DNA G+C content (mol%): 36–38 (Tm and buoyant density). Type strain: ATCC 25175, CCUG 6519, CCUG 11877,CCUG 17824, CIP 103220, DSM 20523, HAMBI 1519,JCM 5705, LMG 14558, NBRC 13955, NCTC 10449. GenBank accession number (16S rRNA gene): AJ243965,AY188348, X58303. Additional comments: The complete genome sequencehas been determined for strain UA159 (GenBank accession no.AE0141333) composed of 2,030,936 bp with a mean G+C content of 36.82 mol%. The genome contains 1963 open reading frames (ORFs) of which 63% were assigned putative functions at the time of publication(Ajdic et al., 2002). Analysis has shown that almost 15% of the genome is accounted for by non-oxidative pathways for carbohydrate metabolism and associated transport systems and reinforces the suggestion that Streptococcus mutans is able to metabolize a wider range of carbohydrates than any other Gram-positive bacterium sequenced to date. A recent publication has described an additional serotype (k) within this species. Serotype k strains were isolated from the dental plaque of children and were less susceptible to phagocytosis than other serotypes (Nakano et al.,2004). 在里面沒看到關(guān)于變異鏈球菌Ingbritt株的描述~而且我在NCBI上查到了Ingbritt相應(yīng)的信息~但是菌種來源直接寫的是變異鏈球菌，沒有具體編號。我發(fā)現(xiàn)中文文獻(xiàn)上有這個~說是變異鏈球菌血清C型~但是確實沒有查到相關(guān)信息。PS：NCBI上變異鏈球菌血清C型顯示的是Streptococcus mutans serotype C，而這個是Streptococcus mutans MT6R。還有，文獻(xiàn)說變異鏈球菌Ingbritt株就是strA基因缺失了11bp核苷酸序列的突變株而已~希望能幫到你~ |
3樓2016-11-15 18:46:33
詩意花香
銅蟲 (初入文壇)
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感謝您的回復(fù)，讓我感受到了一位科研工作者這么專業(yè)、這么認(rèn)真的態(tài)度，雖然仍沒有解決問題，但在態(tài)度上給了我榜樣，謝謝 發(fā)自小木蟲IOS客戶端 |
4樓2016-11-16 09:00:24
jql81070305
新蟲 (小有名氣)
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【答案】應(yīng)助回帖
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見笑了~你說這個菌估計是牙醫(yī)研究的比較多，你可以看看《口腔微生物學(xué)及實用技術(shù)》~我是做食品病原微生物的~之前沒接觸過你說的那個菌~但其實這些東西都是相通的~ UA159其實是ATCC 700610，參見下圖。但是它不是模式菌株，模式菌株是ATCC 25175，見圖2。血清型C的標(biāo)準(zhǔn)菌株有兩個~所以要是說模式菌株，肯定只有這一個~ATCC 25175，要是說血清型C的標(biāo)準(zhǔn)菌株，也就這兩個~ATCC700610（UA159）和700611（UA130）~ 11161.jpg 11162.jpg 11163.jpg 11164.jpg |
5樓2016-11-16 20:23:40
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