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| 以ZERAND為原料,采用酶法提取糖蛋白,在單因素實驗的基礎(chǔ)上采用響應(yīng)曲面法對其提取條件進行優(yōu)化,通過理化分析及UV、IR分析其性質(zhì)和化學(xué)結(jié)構(gòu)特征,并評價其保濕效果。結(jié)果表明:酶法提取糖蛋白的最佳工藝條件是:料液比1:30、酶解時間5h,酶解溫度60 ℃,酶用量5%,酶解pH7.5,最優(yōu)條件下糖蛋白的提取率為0.34%;紅外吸收光譜檢測表明,其具有典型的多糖特征吸收峰,且為含有α糖苷鍵的吡喃糖環(huán)結(jié)構(gòu);該糖蛋白具有良好的吸濕性,優(yōu)于殼聚糖、丙三醇、海藻酸鈉等常規(guī)保濕劑。 |
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以ZERAND為原料,采用酶法提取糖蛋白,在單因素實驗的基礎(chǔ)上采用響應(yīng)曲面法對其提取條件進行優(yōu)化,通過理化分析及UV、IR分析其性質(zhì)和化學(xué)結(jié)構(gòu)特征,并評價其保濕效果。結(jié)果表明:酶法提取糖蛋白的最佳工藝條件是:料液比1:30、酶解時間5h,酶解溫度60 ℃,酶用量5%,酶解pH7.5,最優(yōu)條件下糖蛋白的提取率為0.34%;紅外吸收光譜檢測表明,其具有典型的多糖特征吸收峰,且為含有α糖苷鍵的吡喃糖環(huán)結(jié)構(gòu);該糖蛋白具有良好的吸濕性,優(yōu)于殼聚糖、丙三醇、海藻酸鈉等常規(guī)保濕劑。 Glycoproteins were extracted by enzyme extraction using ZERAND as the starting matieral. The extractoin conditions were optimized based on single factor experiments following response surface methodology. The properties and chemical structures of the extracted glycoproteins were characterized by physical and chemical analysis, UV analysis and IR analysis. In addition, the moisturizing effects of the extracted glycoproteins were evaluated. The experimental results demonstrated that the optimum processing conditions to extract the glycoprotein by enzyme extraction comprised the followings: the solid-liquid ration was 1:30; the enzymolysis time period was 5h; the enzymolysis temperature was 60 ℃; the concentration of the enzyme was 5%; and the pH of enzmolysis was 7.5. The extraction yield of the glycoproteins under the optimum conditions was 0.34%. Moreover, the IR spectrum showed that the extracted glycoprotein had typical characteristic absroption peaks of polysaccride, and comprised a pyranose ring structure having an α glycosidic bond. Finally, the extracted glycoprotein had excellent moisturizing effects, which were superior to those of the conventional humectants, such as chitosan, glycol, and sodium alginate. |
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