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[求助]
關(guān)于轉(zhuǎn)染條件
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最近投了一片論文,其中一個審稿人給了一條意見說轉(zhuǎn)染條件要在10%的血清條件下,我查了下大部分文獻都是在無血清的條件下轉(zhuǎn)染6h小時左右,我是藥劑專業(yè)的,我問了下藥理那邊同學(xué),也說一般在無血清條件下做轉(zhuǎn)染。還有關(guān)于“ PCR data should be normalized to two different genes, which are not affected”這個句話到底是什么意思啊,求大神指點下。 審稿人意見如下: It is not relevant to perform transfection studies under serum-free conditions. In vivo conditions are never serum-free. The authors should report transfection data generated in the presence of 10% serum. PCR data should be normalized to two different genes, which are not affected. As controls in the transfection assays, the nanoparticles encapsulating the control siRNA should be tested to evaluate the effect of the carrier@飛約瘋?cè)嗽?/u>@biostar2009 |
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2、可能希望你能做不相關(guān)、不相互影響的兩個基因的PCR實驗吧,證明PCR實驗條件是標準化的、穩(wěn)定的。 發(fā)自小木蟲Android客戶端 |
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