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wufeiyan2016新蟲 (初入文壇)
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[求助]
光致發(fā)光光譜檢測H2O2 已有1人參與
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| 各路大神,求光致發(fā)光光譜(PL)檢測H2O2的方法 |
木蟲 (正式寫手)
木蟲 (正式寫手)
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Subsequently, hydrogen peroxide (H2O2) was measured using a (p-hydroxyphenyl) acetic acid (POHPAA) analysis method . Typically, a certain volume of fluorescence reagent (potassium hydrogen phthalate: 8.2 g/L, p-hydroxyphenylacetic acid: 270 mg/L, and type II horseradish peroxidase: 30 mg/L) was preadded into the reaction system. 1.0 mL of sample wgas then withdrawn at an interval time of 2 h and then mixed with 1.0 mL of 1.0 mol/L NaOH for 10 min for measuring the intensity of the fluorescence emission at 409 nm excited at 315 nm. 首先你自己要先做一個標(biāo)準(zhǔn)曲線 |
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