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The biological transformation of the biologically active chlorogentisyl alcohol (1), isolated from the marinederived fungus Aspergillus sp., was studied. Preparative-scale fermentation of chlorogentisyl alcohol with marine-derived fungus Chrysosporium synchronum resulted in the isolation of a new glycosidic metabolite, 1-O-(aD-mannopyranosyl)chlorogentisyl alcohol (2). The stereostructure of the new metabolite obtained was assigned on the basis of detailed spectroscopic data analyses, chemical reaction, and chemical synthesis. Compounds 1 and 2 exhibited significant radical-scavenging activity against 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) with IC50 values of 1.0 and 4.7 mM, respectively. The compounds 1 and 2 were more active than the positive control, Lascorbic acid (IC50, 20.0 mM). 捕獲.PNG |
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有機(jī)合成: 金幣+50, 翻譯EPI+1, ★★★★★最佳答案 2017-05-04 17:21:58
有機(jī)合成: 金幣+50, 翻譯EPI+1, ★★★★★最佳答案 2017-05-04 17:21:58
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The biological transformation of the biologically active chlorogentisyl alcohol (1), isolated from the marinederived fungus Aspergillus sp., was studied. Preparative-scale fermentation of chlorogentisyl alcohol with marine-derived fungus Chrysosporium synchronum resulted in the isolation of a new glycosidic metabolite, 1-O-(aD-mannopyranosyl)chlorogentisyl alcohol (2). The stereostructure of the new metabolite obtained was assigned on the basis of detailed spectroscopic data analyses, chemical reaction, and chemical synthesis. Compounds 1 and 2 exhibited significant radical-scavenging activity against 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) with IC50 values of 1.0 and 4.7 mM, respectively. The compounds 1 and 2 were more active than the positive control, Lascorbic acid (IC50, 20.0 mM). 對(duì)從海洋真菌Aspergillus sp分離出的生物活性物質(zhì)氯代龍膽醇(1)的生物轉(zhuǎn)化進(jìn)行了研究。用海洋真菌Chrysosporium synchronum進(jìn)行的制備級(jí)發(fā)酵分離得到新的代謝物糖苷1-O-(aD-mannopyranosyl)chlorogentisyl alcohol (2)，用詳細(xì)的分光光譜數(shù)據(jù)分析，化學(xué)反應(yīng)和化學(xué)合成的方法確定了這一新的代謝物的立體結(jié)構(gòu)�；衔�1和2都表現(xiàn)出顯著的清除自由基1,1-diphenyl-2-picrylhydrazyl radical (DPPH)的活性， IC50值分別為1.0 和 4.7 mM�；衔�1和2的清除自由基活性都比對(duì)照物L(fēng)抗壞血酸 (IC50, 20.0 mM)的強(qiáng)。 |
2樓2017-05-03 10:53:32
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