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627455987木蟲 (小有名氣)
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[求助]
求幫助查詢文章是否被EI收錄，謝謝
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海藻糖合酶在畢赤酵母中的表達(dá)[J]. 現(xiàn)代食品科技,2013,29(11):2575-2579 求幫助查詢文章是否被EI收錄，謝謝！ |
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Expression of trehalose synthase in Pichia pastoris Accession number: 20140417231137 Authors: Wang, Rui-Ming 1 ; Li, Zhong-Kui 1 ; Wang, Teng-Fei 1 ; Liu, Hong-Juan 1 ; Li, Pi-Wu 1 Author affiliation : 1 Department of food and Biology Engineering, QILU university of technology, Jinan 250353, China Corresponding author: Wang, R.-M. Source title: Modern Food Science and Technology Abbreviated source title: Mod. Food Sci. Technol. Volume: 29 Issue: 11 Issue date: 2013 Publication Year: 2013 Pages: 2575-2579 Language: Chinese ISSN: 16739078 Document type: Journal article (JA) Publisher: South China University of Technology, Guangzhou, 510640, China Abstract: The expression vector was constructed from trehalose synthase (TreS) gene(AE015451.1) of Pseudomonas putida to express in Pichia pastoris. TreS gene was amplified by PCR and double digested by EcoRI/XbaI, then linked to the pPICZaA which digested with same enzymes. The gene was identified, sequenced and transformed into pichia pastoris GS115 by electroporation. Positive colonies harboring target genes were screened out by the YPD medium with Zeocin. The genome of positive colonies were extracted and a same size band with target gene was abtained by using PCR which illustrated target gene had successfully transferred to Pichia pastoris. SDS-PAGE and HPLC results showed that the recombinant enzyme had a molecular of 76 ku band which was consistent with the predicted molecular mass, and it had the ability to catalyze the conversion of maltose into trehalose. The recombinant pPICZaA-TreS plasmid successfully constructed and integrated into Pichia pastoris genome and expressed as expected. Number of references: 14 Main heading: Gene expression Controlled terms: Yeast Uncontrolled terms: Electroporation - Expression vectors - Pichia Pastoris - Pichia pastoris gs115 - Pseudomonas putida - Recombinant enzymes - Trehalose - Trehalose synthase Classification code: 461.8.1Genetic Engineering - 801.2Biochemistry Database: Compendex Compilation and indexing terms, © 2017 Elsevier Inc. |
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