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小木蟲論壇-學術科研互動平臺 » 生物醫(yī)藥區(qū) » 分子生物 » PCR相關 » 線粒體DNA
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1125科研王子銀蟲 (初入文壇)
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[求助]
線粒體DNA 已有1人參與
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長鏈pcr的原理是什么？ 發(fā)自小木蟲IOS客戶端 |
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Rose56882
新蟲 (著名寫手)
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2樓2017-11-28 09:36:06
Rose56882
新蟲 (著名寫手)
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Efficient Long PCR results from the use of two polymerases: a non-proofreading polymerase is the main polymerase in the reaction, and a proofreading polymerase (3' to 5' exo) is present at a lower concentration. Following the results of Cheng et al. (1) we have had success using Tth (ABI/Perkin-Elmer) as the main-component polymerase and Vent (New England Biolabs) as the fractional-component polymerase. Other combinations of proofreading and non-proofreading polymerases have been used successfully for many applications. The buffer listed below works well with Tth and Vent, but not with others. If you are interested in using other polymerases make sure that you use compatible buffers. 發(fā)自小木蟲IOS客戶端 |
3樓2017-11-28 09:43:32
Rose56882
新蟲 (著名寫手)
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- 散金: 1306
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- 帖子: 1354
- 在線: 46.5小時
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【答案】應助回帖
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感謝參與，應助指數(shù) +1
1125科研王子: 金幣+500 2017-11-28 09:45:34
silicare: 金幣-1, 違規(guī)存檔, 不要轉(zhuǎn)移金幣，下不為例 2017-11-28 12:05:29
感謝參與，應助指數(shù) +1
1125科研王子: 金幣+500 2017-11-28 09:45:34
silicare: 金幣-1, 違規(guī)存檔, 不要轉(zhuǎn)移金幣，下不為例 2017-11-28 12:05:29
|
Efficient Long PCR results from the use of two polymerases: a non-proofreading polymerase is the main polymerase in the reaction, and a proofreading polymerase (3' to 5' exo) is present at a lower concentration. Following the results of Cheng et al. (1) we have had success using Tth (ABI/Perkin-Elmer) as the main-component polymerase and Vent (New England Biolabs) as the fractional-component polymerase. Other combinations of proofreading and non-proofreading polymerases have been used successfully for many applications. The buffer listed below works well with Tth and Vent, but not with others. If you are interested in using other polymerases make sure that you use compatible buffers. 發(fā)自小木蟲IOS客戶端 |
4樓2017-11-28 09:44:54
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