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江蜜008銅蟲(chóng) (著名寫(xiě)手)
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[交流]
碳管分散液濃度和吸光度的曲線
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看到一篇文獻(xiàn),碳管用SDBS分散,超聲離心后,沉淀水洗幾次凍干稱重,以此計(jì)算得到分散液的濃度,將此分散液逐級(jí)稀釋,繪制濃度和吸光度的標(biāo)準(zhǔn)曲線,整個(gè)過(guò)程重復(fù)五次。那么請(qǐng)教各位大神,重復(fù)五次后是得到五條曲線嗎?那這五條曲線怎么擬合成一條?再問(wèn)問(wèn)文獻(xiàn)的意思是不同的表面活性劑需要做不同的曲線嗎?還是只有一條標(biāo)準(zhǔn)曲線?求大神們看過(guò)來(lái) 附上文獻(xiàn)原文: Preparation of the calibration plot of SWNT dispersion in water: The calibration curve was obtained by plotting the absorbance vs concentration of SWNT in the dispersed state in water. To determine the quantity of nanotubes in dispersed state, SWNT (4mg) was added to an aqueous solution (16 mL) of SDBS (2.5mg/mL) to achieve an initial concentrationof SWNT in the aqueous mixture, 0.25 mg/mL. This aqueous suspension was tip sonicated for 10 min (at 40% power output) followed by bath sonicationfor 2h and tip sonication again for 10 min to prepare the aqueous dispersion of SWNT. Then the dispersion was centrifuged at 2300 g for 90 min. The supernatant was kept aside and used later. While the residue was washed well (3 times) so that it does not contain any trace of SDBS. Finally, theresidue was lyophilized to remove the residual water. The residue was then weighed which was found to be 0.24 mg. Therefore, the total amount of the dispersed SWNT in the supernatant was 0.16 mg i.e., the concentration of SWNT in dispersion was 0.1 mg/mL. For the determination of the absorbance, the optical density of this supernatant was measured at 550 nm through progressive dilution. The dilution was achieved by using SDBS solution (2.5mg/mL) to ensure that similar surfactant concentration is maintained while a successive dilution is obtained for the SWNT dispersions. The absorbance values were plotted as a function of quantity of SWNT in dispersed state. In every case, background correction was performed with the aqueous solution of the respective surfactants in the absence of SWNT. The entire process was repeated for 5 times. |
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至尊木蟲(chóng) (文壇精英)
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