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多浩至尊木蟲 (知名作家)
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[求助]
電化學(xué)檢測(cè)限求助
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各位大神好,請(qǐng)幫忙分析一下關(guān)于檢測(cè)限的問題. 如圖所示為DPV圖和擬合后的圖 Methylene blue can adsorb on ss-DNA and increase its concentration whereas the part of the DNA probe involved in hybridization near the detection limit is very low so that higher number of binding sites would result in lower part of the DNA probe converted to ds-DNA and hence in lower relative decay of the Methylene blue signal. The calculations of LOD could confirm the authors suggestion but they did not take into account the standard deviation of the signal. It was determined as 3% for upper quantification limit of target DNA. This means that at least 10% decay of the Methylene blue signal (S/N=3) would correspond to LOD. As could be seen from the Fig. 8 and Fig. 9, 2 microA shift of the signals corresponds to the target DNA concentration of approx. 10-16 mol/L, 100 times higher than LOD declared. The error bars shown in Fig.9A are much lower than those expected from the S.D. announced. The authors did not provide the description of the Methylene blue signal in blank experiments and this complicates the assessment of the metrological characteristics of the DNA sensor. 請(qǐng)問有沒有大神指點(diǎn)一下審稿人說的是什么意思? 小弟金幣還算多,如能詳細(xì)解釋金幣不在話下'再次感謝 |
新蟲 (初入文壇)
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作者沒有在空白實(shí)驗(yàn)中對(duì)亞甲基藍(lán)信號(hào)進(jìn)行描述,這使得DNA傳感器的計(jì)量特性評(píng)估變得復(fù)雜。 發(fā)自小木蟲IOS客戶端 |
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