 |
 |
 |
| 24小時(shí)熱門(mén)版塊排行榜 |
- >論壇更新日志 (4827)
- >導(dǎo)師招生 (679)
- >考研 (611)
- >蟲(chóng)友互識(shí) (340)
- >文獻(xiàn)求助 (309)
- >休閑灌水 (165)
- >考博 (116)
- >論文投稿 (107)
- >碩博家園 (97)
- >公派出國(guó) (65)
- >博后之家 (63)
- >招聘信息布告欄 (48)
- >基金申請(qǐng) (46)
- >論文道賀祈福 (43)
- >教師之家 (39)
- >SciFinder/Reaxys (32)
 |
 |
 |
||
 |
 |
 |
 |
 |
| 查看: 6714 | 回復(fù): 23 | ||||
[交流]
植物激素提取和測(cè)定方法交流-Elisa和質(zhì)譜方法 已有11人參與
|
||||
|
最近在摸索的植物激素提取和測(cè)定方法，主要是采用液質(zhì)聯(lián)用儀法。在論壇里面看來(lái)很多帖子，自己也查閱了很多文獻(xiàn)，其中pp上有一篇文獻(xiàn)multiple phytohormone profiling by targeted metabolomics中的方法，只需要用20mg新鮮組織就可以提取所有激素。但是需要用到層析柱。 具體方法供大家參考。： For the quantifcation of targeted plant hormones and related compounds, 20-mg (fresh weight) portions of separately harvested roots and shoots were weighed into 2-mL plastic microtubes (Eppendorf) and frozen in liquid nitrogen. To minimize the risk of false-positive detections, possible background levels of analytes were subtracted from measured sample values. Therefore, in the blank controls, 1 mL of extraction buffer containing a mixture of stable isotopically labeled IS also was purifed. Before extraction, three 3-mm ceriastabilized zirconium oxide beads (Next Advance) were added to each sample. A mixture of stable, isotopically labeled IS was added to validate the method and enable the precise quantifcation of endogenous levels of targetedcompounds. The amounts of IS ranged from 0.4 to 50 pmol per sample (precise amounts are listed in Supplemental Table S3). Frozen plant material was extracted in 1 mL of ice-cold 50% aqueous (v/v) ACN using an MM 301vibration mill (Retsch) operating at a frequency of 27 Hz for 5 min. Samples were then sonicated for 3 min at 4°C using a Transsonic T310 ultrasonicator with an ice block-flled bathtub (Elma) and subsequently extracted using a Stuart SB3 benchtop laboratory rotator (Bibby Scientifc) for 30 min at 4°C. After centrifugation (10 min, 36,670 g, and 4°C; Beckman Avanti 30), the supernatant was transferred to clean plastic microtubes. Samples were further purifed according to the scheme shown in Figure 1D. 我們目前參照王學(xué)敏2008年文獻(xiàn)，不使用層析柱，也能測(cè)到大部分激素。 在做激素測(cè)到的同志可以留言一起交流交流。 |
回復(fù)此樓» 收錄本帖的淘帖專(zhuān)輯推薦
 微生物 |
» 猜你喜歡
可以準(zhǔn)確的通過(guò)filecode判斷基金中還是不中。
已經(jīng)有15人回復(fù)
-
感覺(jué)普通人已經(jīng)沒(méi)辦法玩了，躺平是唯一的出路。
已經(jīng)有26人回復(fù)
-
植物學(xué)論文潤(rùn)色/翻譯怎么收費(fèi)?
已經(jīng)有278人回復(fù)
-
江蘇省優(yōu)青門(mén)檻
已經(jīng)有20人回復(fù)
-
心好累。今年我們部門(mén)提交了18個(gè)項(xiàng)目。中了11了，可惜我的又掛了
已經(jīng)有10人回復(fù)
-
面上項(xiàng)目計(jì)劃書(shū)
已經(jīng)有7人回復(fù)
-
面上項(xiàng)目計(jì)劃書(shū)附件
已經(jīng)有9人回復(fù)
-
面上項(xiàng)目計(jì)劃書(shū)系統(tǒng)狀態(tài)
已經(jīng)有18人回復(fù)
-
NSFC-UNEP（與聯(lián)合國(guó)環(huán)境規(guī)劃署）合作項(xiàng)目
已經(jīng)有33人回復(fù)
-
和伊朗人合作
已經(jīng)有32人回復(fù)
-
江蘇省自然基金
已經(jīng)有7人回復(fù)
4樓2018-12-19 09:47:59
3樓2018-12-19 09:14:03
2樓2018-12-19 08:50:57
5樓2018-12-19 10:17:45
6樓2018-12-19 11:49:26
7樓2018-12-19 12:21:39
8樓2018-12-19 13:13:59
9樓2018-12-19 15:00:59
胡哲flight
木蟲(chóng) (小有名氣)
- 應(yīng)助: 0 (幼兒園)
- 金幣: 2281.6
- 帖子: 66
- 在線(xiàn): 19.2小時(shí)
- 蟲(chóng)號(hào): 1137507
- 注冊(cè): 2010-11-02
- 專(zhuān)業(yè): 系統(tǒng)生物學(xué)
10樓2018-12-29 14:56:28
相關(guān)版塊跳轉(zhuǎn)
我要訂閱樓主 iknow 的主題更新
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 285求調(diào)劑 +6 | ytter 2026-03-12 | 6/300 |
|
|---|---|---|---|---|
|
[考研] 材料專(zhuān)碩306英一數(shù)二 +4 | z1z2z3879 2026-03-16 | 4/200 |
|
|
|
[考研] 326求調(diào)劑 +3 | mlpqaz03 2026-03-15 | 3/150 |
|
|
|
[考研] 化學(xué)調(diào)劑0703 +7 | 啊我我的 2026-03-11 | 7/350 |
|
|
|
[考研] 調(diào)劑 +8 | 調(diào)劑的考研學(xué)生 2026-03-09 | 8/400 |
|
|
|
[考研] 0856專(zhuān)碩279求調(diào)劑 +5 | 加油加油！? 2026-03-15 | 5/250 |
|
|
|
[考研] 297一志愿上交085600求調(diào)劑 +5 | 指尖八千里 2026-03-14 | 5/250 |
|
|
|
[考研] 085600求調(diào)劑 +3 | a邵星池 2026-03-09 | 3/150 |
|
|
|
[考研] 0856材料與化工309分求調(diào)劑 +6 | ZyZy…… 2026-03-10 | 6/300 |
|
|
|
[考研] 308求調(diào)劑 +3 | 是Lupa啊 2026-03-10 | 3/150 |
|
|
|
[考研] 321求調(diào)劑 +3 | CUcat 2026-03-10 | 3/150 |
|
|
|
[考研] 327求調(diào)劑 +4 | Ffff03 2026-03-10 | 4/200 |
|
|
|
[考研] 285 求調(diào)劑 資源與環(huán)境 一志愿北京化工大學(xué) +3 | 未名考生 2026-03-10 | 3/150 |
|
|
|
[考研] 0703化學(xué)調(diào)劑 +4 | 快樂(lè)的香蕉 2026-03-11 | 4/200 |
|
|
|
[考研] 336求調(diào)劑 +6 | Iuruoh 2026-03-11 | 6/300 |
|
|
|
[考研] 311求調(diào)劑 +3 | 冬十三 2026-03-13 | 3/150 |
|
|
|
[考研] 【0856】化學(xué)工程（085602）313 分，本科學(xué)科評(píng)估A類(lèi)院�；瘜W(xué)工程與工藝，誠(chéng)求調(diào)劑 +7 | 小劉快快上岸 2026-03-11 | 7/350 |
|
|
|
[考研] 0856化工原理 +6 | z2839474511 2026-03-10 | 6/300 |
|
|
|
[考研] 08食品或輕工求調(diào)劑，本科發(fā)表3篇sci一區(qū)top論文，一志愿南師大食品科學(xué)與工程 +3 | 我是一個(gè)兵， 2026-03-10 | 3/150 |
|
|
|
[考研] 一志愿江南大學(xué)085701環(huán)境工程專(zhuān)碩總分287求調(diào)劑 +5 | 18266118446 2026-03-09 | 5/250 |
|