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【分享】100多種培養(yǎng)基配方！
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1. Acetobacter Medium (醋酸菌培養(yǎng)基) Glucose (葡萄糖) 100g Yeasst extract (酵母膏) 10g CaCO3 20g Agar (瓊脂) 15g Distilled water (蒸餾水) 1000ml Adjust (調(diào)) pH to 6.8 適用范圍：惡臭醋酸桿菌混濁變種 2. Nutrient Agar (營養(yǎng)肉汁瓊脂) Pepton (蛋白胨) 5g Beef extract (牛肉膏) 30g NaCl 5g Agar (瓊脂) 15g Distilled water (蒸餾水) Adjust (調(diào)) pH to 7.0-7.2 [Note]：When cultivation of Bacillus，5mg of to MnSO4.H2O may be added . It is favorable to promote spore formation . 適用范圍：產(chǎn)氣氣桿菌、糞產(chǎn)堿桿菌、蠟狀芽孢桿菌、蠟狀芽孢桿菌蕈狀變種、地衣形芽孢桿菌、巨大芽孢桿菌、多粘芽孢桿菌、塵 埃芽孢桿菌、短小芽孢桿菌、嗜熱脂肪芽孢桿菌、枯草芽孢桿菌、枯草芽孢桿菌深黑變種、蘇云金芽孢桿菌、蘇云金芽孢桿菌蠟螟亞 種(青蟲菌)、蘇云金芽孢桿菌戈爾斯德變種、蘇云金芽孢桿菌猝倒亞種、產(chǎn)氨短桿菌、黃色短桿菌、谷氨酸棒狀桿菌、北京棒桿菌、 大腸埃希氏菌(大腸桿菌)、銅綠假單胞菌(綠膿桿菌)、凸形假單胞桿菌、熒光假單胞菌、彎曲假單胞菌、惡臭假單胞菌、假單胞桿菌、 藤黃八疊球菌、亞黃八疊球菌、尿素八疊球菌、金黃色葡萄球菌、運動發(fā)酵單孢菌 3. Azotobacter Medium (固氮菌培養(yǎng)基) KH2PO4 0.2g K2HPO4 0.8g MgSO4.7H2O 0.2g CaSO4.2H2O 0.1ｇ Na2MoO4.2H2O Trace(微量) Yeast axtract(酵母膏) 0.5g Mannitol(甘露醇) 20g FeCl3 Tract(微量) Distilled water (蒸餾水) 1000ml Agar (瓊脂) 15g Adjust (調(diào)) pH to 7.2 適用范圍：固氮菌、膠質(zhì)芽孢桿菌 4. Corn Meal Medium (玉米粉培養(yǎng)基) Maize flour (玉米粉) 5g Peptone (蛋白胨) 0.1g Glucose (葡萄糖) 1g Tap water (自來水) 1000ml [Note]:Boil the mixture in autoclave at 121℃ for 1 hr. distribute the medium into 18ⅹ18 mm tubes , each contains 10 ml of the li quid , then autoclave at 121℃ for 1 hr . again (15磅蒸煮1小時,分裝入18ⅹ18毫米試管,每管深度達6厘米。15磅再次滅菌15小時。) 5. Lactic-bacteria Medium I (乳酸菌培養(yǎng)基I ) Yeast extract (酵母膏) 7.5g Peptone (蛋白胨) 7.5g Glucose (葡萄糖) 10g KH2PO4 2g Tomato juice (西紅柿汁) 100ml Tween (吐溫) 80 0.5ml Distilled water (蒸餾水) 900ml pH 7.0 適用范圍：植物乳桿菌(胚芽乳桿菌)、嗜熱乳酸鏈球菌 6. Lactic-bacteria Midium Ⅱ (乳酸菌培養(yǎng)基Ⅱ) Lacto-casein peptone (乳酪蛋白胨) 10g Beef extract (蛋白胨) 10g Yeast extract (酵母膏) 5g Glucose (葡萄糖) 5g Tween (吐溫) 80 1g K2HPO4 2g Na-acetate (醋酸鈉) 5g Diamine citrate (檸檬酸二胺) 2g MgSO4.7H2O 0.2g MnSO4.H2O 0.05g Distilled water (蒸餾水) 1000m pH 6.5-6.8 適用范圍：植物乳桿菌(胚芽乳桿菌) 7. Peotone Glucose Yeast extract Medium PGY (蛋白胨、酵母膏、葡萄糖培養(yǎng)基) Peptone(蛋白胨) 10g Yeast extract (酵母膏) 5g Glucose (葡萄糖) 1g Distilled water (蒸餾水) 1L 8. Glycerol Agar (甘油瓊脂) Peptone (蛋白胨) 5g Beef extract (酵母膏) 3g Glycerol (甘油) 20g Top water (自來水) 1000ml Agar (瓊脂) 15g pH 7.0-7.2 9. Rhizobium medium (根瘤菌培養(yǎng)基)AS 9 Yeast eztract (酵母膏) 1g Soil eztract (土壤浸提液) 200ml Mannitol (甘露醇) 10g Agar (瓊脂) 15g Distilled water (蒸餾水) 800ml pH 7.2 [Note]：Soil extract：Suspend 50g finely and dried gardon soil in 200ml of tap water. Autoclave at 121℃ for 1 hr. Decant through cotton-cloth, filler though paper, make up volume to 200ml. Resterilize for 20 minutes at 121℃ , then mixed with othringredients an d distributed. (土壤浸提液的制法：取土壤50克，加水200毫升，15磅蒸煮1小時，經(jīng)濾紙過濾后加水補足到200毫升。) 適用范圍：大豆根瘤菌(慢生型)、豇豆慢生根瘤菌、花生根瘤菌、紫云英根瘤菌、 大豆根瘤菌(快生型)、大豆根瘤菌、豌豆根瘤菌、苜蓿根瘤菌、田菁根瘤菌 10. Mannitol Agar (甘露醇瓊脂) Yeast extract (酵母膏) 5g Peptone (蛋白胨) 3g Mannitol (甘露醇) 25g Agar (瓊脂) 15g Distilled water (蒸餾水) 1000ml 11. Glucose Asparagine (葡萄糖、天門冬素瓊脂) Glucose (葡萄糖) 10g Asparagine (天門冬素) 0.5g K2HPO4 0.5g Water (水) 1000ml pH 7.2-7.4 適用范圍：刺孢小單孢菌絳紅變種、紫色小單孢菌(絳紅小單孢菌) 12. Gause′s Synthetic Agar (高氏合成一號瓊脂) KNO3 1g Soluble starch(可溶性淀粉) 20g K2HPO4 0.5g MgSO4.7H2O 0.5g NaCl 0.5g FeSO4 0.01g Agar (瓊脂) 20g water (水) 1000ml pH 7.2-7.4 適用范圍：刺孢小單孢菌絳紅變種、紫色小單孢菌(絳紅小單孢菌)、白黃鏈霉菌、白色鏈霉菌、抗生鏈霉菌、雙重輪絲鏈霉菌、產(chǎn)色 鏈霉菌、燼灰鏈霉菌、天藍色鏈霉菌、滅蚊鏈霉菌、紅霉素鏈霉菌、青色鏈霉菌、球孢鏈霉菌、淺灰鏈霉菌、灰色鏈霉菌、吸水鏈霉 菌、淡紫灰鏈霉菌、黃色長孢鏈霉菌、藤黃色鏈霉菌、細黃鏈霉菌、黑化鏈霉菌、玫瑰色鏈霉菌、華美鏈霉菌、嗜熱鏈霉菌、委內(nèi)瑞 拉鏈霉菌、紫色直絲鏈霉菌、紫色鏈霉菌、綠色鏈霉菌 13. Wort Agar (麥芽汁瓊脂) Dilute the world (without hop) to 12 Brix. Add 15g agar into 1000ml of the diluted word..Melt the agar by heating, then di stribute the medium into tubes. Autoclave at 110 for 30 minutes. (將發(fā)酵啤酒的原料(未加酒花)，稀釋至12柏林，加瓊脂15克， 溶化后分裝。15 磅滅菌30分鐘。) 適用范圍：克魯斯假絲酵母、郎比可假絲酵母、解脂假絲酵母、馬其頓假絲酵母、擬熱帶假絲酵母、粗壯假絲酵母、皺褶假絲酵母、 熱帶假絲酵母、產(chǎn)朊假絲酵母、阿舒假囊酵母、白地霉、果香地霉、地霉屬、異常漢遜酵母、異常漢遜酵母變種、阿拉伯糖醇漢遜酵 母、施氏漢遜酵母、菅囊酵母、伯頓畢赤酵母、膜醭畢赤酵母、粘紅酵母、小紅酵母、膠紅酵母、深紅酵母、卡爾斯伯酵母、釀酒酵 母、椰園釀酒酵母、發(fā)酵性酵母、洛格酵母、羅斯酵母、魯氏酵母多形魯氏酵母、威爾酵母、酵母、路德類酵母、栗酒裂殖酵母、擲 抱酵母、貝雷絲孢酵母、皮狀絲孢酵母、糙孢曲霉、無殼曲霉、鮮橙曲霉、紅曲霉、紫紅曲霉、紅色紅曲霉、紅曲霉菌 14. Potato Dextrose Agar PDA (馬鈴薯、葡萄糖瓊脂) Potato extract (馬鈴薯汁) 1000ml Dextrose(glucose) (葡萄糖) 20g Agar (瓊脂) 20g [Note]: Potato extract: Slice potatoes thin. Add 1000ml of water, immediately to prevent Oxidtion of juice and boil until sof t. (approximately 30min.). Filter through cotton-cloth. Autoclave at 115℃ for 20 minutes.(注：取去皮馬鈴薯200克，切成小塊， 加水1000毫升煮沸30分鐘，濾去馬鈴薯塊，將濾液補足至1000毫升，加葡萄糖20克，瓊脂15克，溶化后分裝，15磅滅菌30分 鐘。) 適用范圍：酵米面假單胞菌(酵米面黃桿菌)、白色鏈霉菌、燼灰鏈霉菌、青色鏈霉 菌、球孢鏈霉菌、灰色鏈霉菌、龜裂鏈霉菌、傘枝梨頭霉、雅致放射毛霉、棒曲霉、 米曲霉、出芽短梗霉、白僵霉、灰葡萄孢、頂頭孢霉、巴西毛殼、長刺毛殼、橄欖包 毛殼、反曲毛殼、琥珀毛殼、圓酵毛殼、束狀刺盤孢、新月彎孢霉、奇異翅孢殼、地 生翅孢殼、串珠鐮孢、尖鐮孢、盤長孢菌(魯保一號)、銀白楊盤長孢、玉蜀黍長蠕 孢、深黃被孢霉、小被孢霉、多頭被孢、拉曼被孢霉、葡萄色被孢霉、生香毛霉、卷 枝毛霉、兩型孢毛霉、直立毛霉、球孢毛霉、絲球毛霉、大毛霉、多型孢毛霉、總狀 毛霉、魯氏毛霉、五通橋毛霉、黑球漆斑菌、露濕漆斑菌、玫煙色擬青霉、棉鈴蟲擬 青霉、擬青霉、球形阜孢、白腐菌、少根根霉、華根霉、科恩根霉、戴爾根霉、日本根霉、爪哇根霉、米根霉、點頭根霉、綠穂霉、 簇孢匍柄霉、雅致枝霉、康寧木霉、綠色木霉、黃萎輪枝孢、大麗花輪枝孢 15. Czapek′s Agar(察氏瓊脂) Sucrose (蔗糖) 30g NaNO3 3g MgSO4.7H2O 0.5g KCl 0.5g FeSO4.7H2O 0.01g K2HPO4 1g Agar (瓊脂) 13g Distilled water (蒸餾水) 1000ml pH 7.2 適用范圍：紅霉素鏈霉菌、洋蔥曲霉、金頭曲霉、泡盛曲霉、泡盛曲霉煙色變種、亮白曲霉、炭黑曲霉、鹿皮色曲霉、棒曲霉、無花 果曲霉、黃柄曲霉、黃曲霉、煙曲霉、構(gòu)巢曲霉、黑曲霉、赭曲霉、米曲霉、寄生曲霉、醬油曲霉、土曲霉、宇佐美曲霉、焦曲霉、 雜色曲霉、溫特曲霉、阿達青霉、產(chǎn)黃青霉、黃綠青霉、桔青霉、頂青霉、園弧青霉、繩狀青霉、灰黃青霉、島青霉、淡紫青霉、特 異青霉、赭綠青霉、產(chǎn)紫青霉、婁地青霉、小刺青霉、蕁麻青霉、 16. Concentrated Sucrose Czapek′s (濃糖察氏瓊脂) Sucrose (蔗糖) 200g NaNO3 3g MgSO4.7H2O 0.5g KCl 0.5g FeSO4.4H2O 0.01g K2HPO4 1g Agar (瓊脂) 13g Distilled water (蒸餾水) 1000ml 適用范圍：謝瓦曲霉 17. Synthetic Potato Medium (綜合馬鈴薯培養(yǎng)基) 20%Potato extract (馬鈴薯汁) 1L Glucose (葡萄糖) 20g KH2PO4 3g MgSO4.7H2O 1.5g Vitanib B1 (硫胺素) Trace (微量) Agar (瓊脂) 15g pH 6 適用范圍：米曲霉、醬油曲霉、棲土曲霉、宇佐美曲霉、出芽短梗霉、白僵霉、灰葡萄孢、頂頭孢霉、巴西毛客、長刺毛殼、球毛殼、 橄欖包毛殼、反曲毛殼、琥珀毛殼、圓酵毛殼、多主枝孢、束狀刺盤孢、葫蘆科刺盤孢、新月彎孢霉、奇異翅孢殼、地生翅孢殼、串 珠鐮孢、蝕脈鐮孢霉、盤長孢菌(魯保一號)、銀白楊盤長孢、瓶毛殼、金龜子綠僵菌、生香毛霉、卷枝毛霉、兩型孢毛霉、直立毛霉、 球孢毛霉、絲球毛霉、大毛霉、多型孢毛霉、總狀毛霉、魯氏毛霉、五通橋毛霉、黑球漆斑菌、露濕漆斑菌、玫煙色擬青霉、棉鈴蟲 擬青霉、淡紫色擬青霉、擬青霉、白腐菌、少根根霉、根霉屬的菌、華根霉、科恩根霉、戴爾根霉、臺灣根霉、日本根霉、爪哇根霉、 黑根霉、雪白根霉、少根根霉、米根霉、點頭根霉、三寶壟根霉、根霉屬的種、綠穂霉、康寧木霉、木素木霉、擬康氏木霉、木霉、 粉紅木霉、綠色木霉、黃萎輪枝孢、大麗花輪枝孢、雙孢蘑菇、大肥菇、發(fā)光假蜜環(huán)菌、木耳、皺木耳、毛木耳、大禿馬勃、長根菇、 金針菇、棘托竹蓀、短裙竹蓀、短裙竹蓀東北變種(暫定)、長裙竹蓀、白鬼筆、無裙竹蓀、寬鱗大孔菌、凸圓靈芝(白芝)、紫芝、靈芝 (紅芝，赤芝，日本靈芝，韓國靈芝)、灰樹花、猴頭菌、香菇、粗毛斗菇、潔麗香菇、豹皮菇、玉蕈、真姬菇、安絡(luò)小皮傘菌、鬼毛 針、黑柄皮傘菌、尖頂羊肚菌、圓錐羊肚菌、高羊肚菌、黑脈羊肚菌、羊肚菌、滑菇、光帽黃傘、尖鱗黃傘、金頂側(cè)耳、榆黃磨、金 頂磨、白黃側(cè)耳、紫孢平菇、姬菇、裂皮側(cè)耳、擬囊體側(cè)耳(鮑魚菇)、真線側(cè)耳短孢變種、真線側(cè)耳、扇形平菇、扁形平菇、佛羅里 達無孢子側(cè)耳、佛羅里達側(cè)耳、蠔菇、糙皮側(cè)耳、平菇、貝形側(cè)耳、鷹翅菌、肺形側(cè)耳、柳樹菌、樹窩、扁柄側(cè)耳、美味側(cè)耳、紫孢 側(cè)耳、粉褶側(cè)耳、粉紅側(cè)耳、鳳尾菇、漏斗狀側(cè)耳、榆干側(cè)耳、大榆磨、側(cè)耳、茯苓、黑菇、煙色紅菇、裂褶菌、雞縱、金耳、黃木 耳、銀耳、白木耳、紫晶口磨、銀絲菇、絲蓋小包腳菇、草菇 18. Corn Meal Medium (玉米粉培養(yǎng)基) Mix corn meal with water [20:100(W/V)] well . Distribute the mixture into 100ml bottles , each bottle contains about 30ml of the mixture . Autoclave at 121℃ for 30minutes . (20%玉米粉裝瓶，15 磅滅菌30分鐘。) 19. Filter Paper Medium (濾紙培養(yǎng)基) (NH4)2SO4 1g KH2PO4 1g MgSO4.7H2O 0.7g NaCl 0.5g Distilled water (蒸餾水) 1000ml A strip of filter paper (濾紙條) 6ⅹ1cm (厘米) 20. Soybean Cake Meal Agar (黃豆餅粉瓊脂) 10% infusion of soybean cake meal (10%黃豆餅粉浸汁) 1000ml Glucose (葡萄糖) 10g NaCl 2.5g 21. Sawdust， Wheat-bran Medium (木屑、麩皮培養(yǎng)基) Sawdust of broad leaf tree 75g Wheat bran 25g Preparation：Mix these two ingredients well . Add appropriate water and blend (the amount of water added may be becided by kneading with fingers ). Fill the mixture into broad mouth of bottles . Stick a hole on the filling, put the cotton pulg in bottle mouth . 殼斗科植物或闊葉樹的木屑75克 麩皮 25克 制法：加水適量充分混勻，水量以手捏時指間有水珠為宜，裝瓶并壓緊，在中心處扎一小洞，堵上棉塞，20磅滅菌1小時。 22. Pine Block or Pine Sowdust Medium (松木條或松木屑培養(yǎng)基) 1. Cut 1021 cm pine biocks and immerse them in 1-2% sucrose solution . Alow the biocks fully absorbing suger solution .Fill the biocks into broad month of bottle . Park the bottle with paper . Autoclave at 20 lbs for 1 hr . (將10ⅹ2ⅹ1厘米的松木條浸泡在1-2%的蔗糖 水中。使木條吸足糖水，裝瓶不宜過緊，用紙包好，20磅滅菌1小時。) 2. Pine Sawdust Medium Pine Sawdust (松木屑) 75% Rice bran (米糠) 23-24% Socrose (蔗糖) 1-2% Water (appropriate)水 (適量) Autoclave at 20 lbs for 1 hr.(20 磅滅菌1小時) 23. Rice Straw. Dung Medium (稻草、馬糞培養(yǎng)基) Dried horse dung (or cow dung) (干馬糞或牛糞) 65% Died straw (干稻草) 35% Urea (尿素) 0.5% Calcium superhosphate (過磷酸鈣)0.7% Gypsum powder(石膏粉) 1% Ammonium sulphate (硫酸銨) 0.3% [Note]: Add appropriate water, pile them up and cause them fermentation. (水適量進行堆積發(fā)酵) 24. Straw Rice Bran Medium (稻草、米糠培養(yǎng)基) Dried straw (干稻草) 75-80% Rice bran (米糠) 20-25% [Note]：Select no mouldy straw and cut them about 3 cm in length. Immerse them in fresh water for 12 hr. Allow the straw fully (the amount of water added may be decided by kneading with fingers. It is right when a drop of water appeared between fingers). ( [注]： 選無霉稻草，切成長約3 厘米的小段，用清水浸泡12小時，使充分吸水，然后傾去水，加米糠和水，水量以用手捏時指間有水珠為宜。) 25. Rice Medium (米飯培養(yǎng)基) Fill 10g of rice into a 50ml of Erlenmeyer flask. Add 20-30ml water. Autoclave at 121℃ for 30 minutes (or fill 2g of rice into a 15ⅹ 150mm tube). Add 6 ml of water. Autoclave at 121℃ for 30 minutes. (取大米10克，入50毫升椎形瓶中，水20-30毫升于15 磅滅 菌30分鐘即成，或取大米2克，裝入15ⅹ150 毫米試管中，加水6 毫升，于15磅滅菌30分鐘。) 26. Filter Paper Medium (濾紙條培養(yǎng)基) (NH4)2SO4 1g KH2PO4 1g MgSO4.7H2O 0.5g Yeast Extract (酵母膏) 0.1g Distilled Water (蒸餾水) 1000ml Strip of filter paper 1ⅹ7 cm (濾紙條 1ⅹ7 厘米) [Note]：Siddolve the above ingredients. Distribute the liqid medium into tubes (each tube contains 1ml of the broth). Autoclave at 121 ℃ for 30 minutes. Then put one or two sterilized strips of filter paper into a tube aseptically. (將上述溶液分配成溶液后分裝入試管， 每管1 毫升，15磅滅菌30分鐘。再將滅菌的濾紙條(1條或2條)投入試管中即好使用。) 27. Yeast Extract-Medium Extract Agar I (酵母膏、麥芽膏瓊脂) Yeast extract (酵母膏) 10g Mall extract (麥芽膏) 10g Glucose (葡萄糖) 4g Distilled water (蒸餾水) 1000ml Agar (瓊脂) 20g pH 7.0 適用范圍：紅霉素鏈霉菌 28. Yeast Extract Peptone (酵母膏、蛋白胨瓊脂) Yeast extract (酵母膏) 1g Multi-peptone(多蛋白胨) 2g Beef extract (牛肉膏) 1g Glucose (葡萄糖) 10g Agar (瓊脂) 20g Distilled water (蒸餾水) 1L pH 7.0 |
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41. Thiobacillus Ferrooxidans Medium (氧化亞鐵硫桿菌培養(yǎng)基) (NH4)2SO4 0.15g KH2PO4 0.05g KCl 0.05g MgSO4.7H2O 0.5g Ca(NO3)2.4H2O 0.01g FeSO4.7H2O 50g Distilled water (蒸餾水) 1000ml pH 2.0 42. Thiobacillus Thiooxidans Medium (氧化硫硫桿菌培養(yǎng)基) (NH4)2SO4 0.3g KH2PO4 3-4g CaCl2 (anhydrous) [無水] 0.25g MgSO4.7H2O 0.5g FeSO4.7H2O 0.001g Distilled water (蒸餾水) 1000ml pH 3.5-4 43. Plasma Substitute Medium (代血漿培養(yǎng)基) Sucrose (蔗糖) 12.5-15% Peptone (蛋白胨) 0.5% KH2PO4 0.03% Na2HPO4.12H2O 0.14% pH 7.0-7.2 44. Skim Milk Medium (脫脂牛奶培養(yǎng)基) ATCC 183 脫脂牛奶100g 去離子水1000ml 適用范圍：保加利亞乳桿菌、嗜熱乳酸鏈球菌 45. Pea Agar (豌豆瓊脂) Glucose (葡萄糖) 1% Peptone (蛋白胨) 0.5% NaCl 0.5% Agar (瓊脂) 2.0% Pea infusion (counted by NH2) [豌豆浸汁(以NH2計)] 12-14 mg∕100 pH (before sterilization) [消前] 7.2-7.5 [Note]: Preparetion of pea infusion (豌豆浸汁的制法) : Preparetion 900g of pea. Put them into a 5L bottle, and add 3L of tap water. Adjust pH to 4.3-4.5 by using of sulphuric acid. Add 30ml of chloroform for antisepsis. Plug with rubber stopper and bind up the bottle mouth with gauge in order to avoid that the stopper fall off due to gas pressure. Place the bottle at 37℃ for 7-10 days. Filter the infusion, then boil the filtrate to escape the chloroform. Filter again Distribution and autoclave at 120℃ for 30 minutes. Store at cool contition . (稱取白豌豆900克，置于5公升廣口瓶中，加自 來水3 公升，用硫酸調(diào)pH至4.3-4.5，再加氯仿30毫升(防腐)，加膠塞并用紗布扎口(防止沖脫)，放37℃恒溫室7-10天。取出用濾 紙過濾，濾液煮沸20分鐘，以除氯仿，并再次以濾紙過濾，除沉淀。濾液分裝，于120℃30分鐘滅菌。后冷藏備用。) The standard quality of pea infusion as following (豌豆浸汁質(zhì)量標(biāo)準(zhǔn))： Aminonitrogen(氨基酸): 160-200 mg∕100ml Soluble phosphoruse(溶磷): 400-600 μg∕ml Color(色澤): auratus,transparent (金黃、透明) 46. Wheat Bran Medium (麥麩瓊脂) Wheat bran (麥麩) 3.6% (NH4)2HPO4 0.3% K2HPO4 0.02% MgSO4.7H2O 0.01% Agar (瓊脂) 2% pH 7.0 適用范圍：金霉素鏈霉菌、紅霉素鏈霉菌 47. Glucose Yeast Extract Agar (葡萄糖、酵母膏瓊脂) Glucose (葡萄糖) 10g Yeast extract (酵母膏) 10g Water (水) 1000ml Agar (瓊脂) 15g pH 7.2 48. MMN Medium (MMN培養(yǎng)基) CaCl2 0.05g MgSO4 0.15g NaCl 0.025g FeCl3 (1%) 1.2ml KH2PO4 0.5g Vitamib B1 (硫胺素) 100μg (NH4)2HPO4 0.25g Wort (12 Be′) 100ml Glucose (葡萄糖) 10-15g Citric acid (檸檬酸) 0.2g Distilled water (蒸餾水) 900ml pH 5.5 (左右) 適用范圍：蜜環(huán)菌49. Starch Agar (淀粉瓊脂) Soluble starch (可溶性淀粉) 10g NaNO3 1g MgCO3 1g K2HPO4 0.3g NaCl 0.5g Agar (瓊脂) 20g Distilled water (蒸餾水) 1000ml 50. 5 Be′Wort Agar (5 Be′麥芽汁瓊脂) 51. 5 Be′Koji Extract Agar (5 Be′米曲汁瓊脂) 適用范圍：華根霉、三寶壟根霉 52. 發(fā)根農(nóng)桿菌培養(yǎng)基(YEB) Beef extract (牛肉浸膏) 5g/L Yeast extract (酵母膏) 1g/L Peptone (蛋白胨) 5g/L Sucrose (蔗糖) 5g/L MgSO4.7H2O 0.4g/100ml Agar (瓊脂) 1.5g/100ml pH 7.4 適用范圍:青枯假單胞菌 53. 青枯菌培養(yǎng)基(Tm) Peptone (蛋白胨) 10g Casein (干酪素水解物產(chǎn)) 1g Glucose (葡萄糖) 5g Ddhro (純水) 1000ml Agar(瓊脂) 15g pH 7.0 54. YM (Yeast Malt Agar) 酵母膏3.0g 麥芽汁3.0g 蛋白胨5.0g 瓊脂20.0g 蒸餾水1000ml 55. ISP 4 可溶性淀粉10.0g K2HPO4 1.0g MgSO4.7H2O 1.0g NaCl 1.0g (NH4)2SO4 2.0g CaCO3 2.0g FeSO4.7H2O 0.001g MnCl2.7H2O 0.001g 瓊脂 20.0g 蒸餾水1000ml 56. Trypton Soy Agar 胰蛋白胨17.0g 大豆胨3.0g 葡萄糖2.5g 瓊脂20.0g NaCl 5.0g K2HPO4 2.5g 蒸餾水1000ml 57. NA (Nutrient Agar) 牛肉膏3.0g 蛋白胨5.0g 瓊脂 20.0g 蒸餾水1000ml pH 6.6-7.0 58. 甘露(糖)醇瓊脂(ATCC 1) 酵母膏5.0g 蛋白胨3.0g 甘露(糖)醇25.0g 瓊脂15.0g 蒸餾水1000ml 59. Marine瓊脂2216 (ATCC 2) 蛋白胨5.0g 酵母膏1.0g 檸檬酸鐵0.1g 溴化鉀0.08g NaCl 19.45g MgCl2 8.8g Na2SO4 3.24g CaCl2 1.8g KCl 0.55g NaHCO3 0.16g NH4NO3 0.0016g Na2HPO4 0.008g 氯化鍶0.034g 硼酸0.022g 硅酸鈉0.004g 氟化鈉0.0024g 瓊脂 15.0g 蒸餾水1000ml pH 7.4-7.8 |
3樓2010-04-22 13:44:26
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60. 兔血瓊脂(ATCC 4) 牛心(取其浸液) 500.0g 胰蛋白胨(Tryptose) 10.0g NaCl 5.0g 瓊脂15.0g 蒸餾水1000ml pH 6.6-7.0 61. 孢子形成培養(yǎng)基(ATCC 5) 酵母膏1.0g 牛肉膏1.0g 胰蛋白胨2.0g 葡萄糖10.0g FeSO4 微量瓊脂 15.0g 蒸餾水1000ml pH 7.2 62. Trypticase Soy Agar (ATCC 18) Trypticase Soy Broth 30.0g 瓊脂15.0g 蒸餾水1000ml 63. YPAD (ATCC 109) 酵母膏10.0g 蛋白胨20.0g 葡萄糖20.0g 瓊脂20.0g 蒸餾水1000ml pH 7.0 64. SP (ATCC 432) 棉子糖1.0g 蔗糖1.0g 半乳糖1g 可溶性淀粉5g 水解酪蛋白2.5g 瓊脂20.0g MgSO4.7H2O 0.5g K2HPO4 0.25g 蒸餾水1000ml pH 自然 65. 胰酪胨大豆酵母浸膏瓊脂(TSA-YE) 胰蛋白胨15.5g 大豆蛋白胨5.0g NaCl 5.0g 酵母膏6.5g 瓊脂 15.0g 蒸餾水1000ml pH 7.3 66. ATCC 20 蛋白胨5.0g 酵母膏15.0g K2HPO4 3.0g 葡萄糖2.0g 瓊脂 20.0g 蒸餾水1000ml pH 7.3-7.5 67. TY (Yeast Tryptone Agar) 蛋白胨10.0g 酵母膏5.0g 葡萄糖1.0g NaCl 5.0g 瓊脂 20.0g 蒸餾水1000ml pH 7.0 68. 硫醇(Thiol)培養(yǎng)基(ATCC 49) 示蛋白胨(Proteose Peptone No.3,Difco) 10.0g 酵母膏5.0g 葡萄糖1.0g NaCl 5.0g 硫醇復(fù)合物8.0g 瓊脂1.0g p-氨基苯甲酸0.05g pH 6.9-7.3 69. Czapek＇Dox 瓊脂培養(yǎng)基(ATCC 134) Czapek＇Dox Broth 35.0g 瓊脂15.0g 蒸餾水1000ml 70. MG/L 蛋白胨5.0g 甘露醇5.0g 谷氨酸鈉1.15g 生物素0.0001g K2HPO4 0.25g NaCl 0.1g MgSO4.7H2O 0.1g 酵母膏2.5g 瓊脂 20.0g 蒸餾水1000ml 氯霉素(終濃度) 50μg/ml pH 7.0 71. Eclb Broth 蛋白胨10.0g 酵母膏5.0g NaCl 5.0g 瓊脂20.0g 蒸餾水1000ml 氨芐青霉素(終濃度) 300μg/ml pH 7.0 72. Bennetts Agar (IFO 228) 酵母膏1.0g 牛肉膏1.0g NZ Amine,type A 2.0g 葡萄糖10.0g 瓊脂 20.0g 蒸餾水1000ml pH 7.3 73. Harroldˊs M40Y (ATCC 319) 麥芽膏20.0g 酵母膏5.0g 蔗糖400.0g 瓊脂20.0g 蒸餾水1000ml 74. PSA (Potato Sucrose Agar) 土豆200g 蔗糖20g 蒸餾水1000ml 瓊脂20g pH 6.0-6.5 75. 培養(yǎng)基 牛肉膏300g 水解酪蛋白17.5g 可溶性淀粉1.5g 瓊脂20.0g 蒸餾水1000ml 磺胺嘧啶 (終濃度) 100μg/ml 氨芐青霉素 (終濃度) 100μg/ml pH 7.3-7.4 76. 馬鈴薯牛肉汁培養(yǎng)基 麥芽糖20.0g 牛肉膏5.0g 蛋白胨3.0g 馬鈴薯200.0g pH 7.0-7.2 77. ISP 2 酵母膏4.0g 麥芽汁10.0g 葡萄糖4.0g 瓊脂20.0g 蒸餾水1000ml pH 7.0 78. BHI (Brain Heart Infusion) 牛腦200.0g 牛心浸出汁250.0g 蛋白胨10.0g 葡萄糖2.0g NaCl 5.0g 瓊脂20.0g 蒸餾水1000ml pH 6.8-7.2 79. YPG (Yeast Peptone Glucose) 酵母膏10.0g 蛋白胨20.0g 葡萄糖20.0g 瓊脂20.0g 蒸餾水1000ml pH 7.0 |
4樓2010-04-22 13:44:48
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80. 兔食瓊脂培養(yǎng)基(ATCC 340) 兔食(商店出售) 25.0g 瓊脂20.0g 蒸餾水1000ml *煮沸兔食，并浸泡1/2小時，用多層紗布過濾，加瓊脂于濾液中。 81. 土豆瓊脂(ATCC 423) 200 克去皮土豆切成小塊于1000ml 自來水中煮沸1 小時,用細棉布過濾.加進5.0mgMnSO4,pH 調(diào)至6.8,用自來水定容至1000ml。加 進15.0g瓊脂，溶化，分裝并滅菌。 82. 土豆浸汁加無機鹽培養(yǎng)基(ATCC 470) 土豆2000g MnSO4.5H2O 0.02g MgSO4.7H2O 0.4g ZnSO4.7H2O 0.01g CuSO4. 5H2O 0.01g FeSO4.7H2O 0.1g CaCl2.2H2O 0.1g K2HPO4 0.5g * 稱取200g去皮塊狀土豆,加入1000ml 蒸餾水煮沸30分鐘,多層紗布過濾,濾液中加入以上成分,溶解后定容至1000ml。 83. 無機鹽淀粉瓊脂培養(yǎng)基(ATCC 527) 可溶性淀粉10.0g K2HPO4 1.0g MgSO4 1.0g NaCl 1.0g (NH4)2SO4 2.0g CaCO3 2.0g FeSO4.7H2O 0.001g MnCl2.7H2O 0.001g ZnSO4.7H2O 0.001g 瓊脂20.0g pH 7.0-7.4 84. (AS125) 新鮮雞蛋15-16 蒸餾水375ml 2%孔雀(石)綠10.0g K4HPO4 1.5g MgSO4.7H2O 0.375g 天冬酰胺2.25g 甘油 7.5ml 土豆粉18.8g 85. 燕麥片(Oatmeal)瓊脂培養(yǎng)基 燕麥片60.0g 瓊脂12.5g 蒸餾水1000ml * 將燕麥片加600ml水中，制成勻漿，加熱至45-50oC，然后加入已溶化瓊脂的另400ml水，121oC滅菌90分鐘。 86. 細菌培養(yǎng)基(ATCC 573) (NH4)2SO4 1.3g K2HPO4 0.37g MgSO4.7H2O 0.25g CaCl2.2H2O 0.07g FeCl3 0.02g 葡萄糖1.0g 酵母膏1.0g 蒸餾水1000ml 用10N H2SO4 調(diào)pH 至4.0。若配制固體培養(yǎng)基，為增強上述溶液的強度，pH 需調(diào)至3.5，瓊脂水溶液濃度為40g/L。兩者分別高壓 滅菌，冷卻至約50oC，無菌操作將兩種溶液等體積混合，這一過程可避免瓊脂被酸水解。 87. 碎肉培養(yǎng)基(ATCC 593) 牛肉(去脂肪) 500.0g 蒸餾水1000ml NaOH 25.0ml * 所用瘦牛肉或馬肉(碾)磨碎前需去脂肪和周圍的組織。將肉、水、NaOH混合后邊攪拌邊加熱，冷至室溫，去掉表面油脂，過濾，留 下肉末和濾液。濾液加蒸餾水至1000ml。濾液中加下列成分： 蛋白胨30.0g 酵母膏5.0g K2HPO4 5.0g 0.025%刃天青(Resazurin)溶液4.0ml 然后煮沸，冷卻，加0.5g L-半胱氨酸HCl。H2O，pH調(diào)至7.0。在氧氣、氮氣(O2-freenitrogen)和3%氫氣條件下，按1份碎肉加4-5 份以上溶液的比例分裝于每支試管。在氮氣和氫氣條件下，使用橡皮塞，快排氣，高壓滅菌15分鐘。 88. YMPG 酵母膏3.0g 麥芽汁3.0g 蛋白胨5.0g 葡萄糖10.0g FeSO4.7H2O 0.1g 瓊脂20.0g 蒸餾水1000ml pH 6.8 滅菌：110oC，35分鐘 89. TGYM培養(yǎng)基(ATCC 679) 胰蛋白胨5.0g 葡萄糖1.0g 酵母膏3.0g 蒸餾水1000ml DL-蛋氨酸(DL-Meth 0.5g 90. TYG培養(yǎng)基(ATCC 741) 胰蛋白胨3.0g 酵母膏3.0g 葡萄糖3.0g K2HPO4 1.0g 瓊脂 20.0g 蒸餾水1000ml pH 7.4 91. M9 Na2HPO4 12.8g KH2PO4 3.0g NaCl 0.5g NH4Cl 0.5g 葡萄糖(單獨滅菌) 10.0g 維生素B1(單獨滅菌) 1μg/ml pH 自然 92. ISP3 (IFO 245) 燕麥粉20.0g 微量元素溶液1ml pH 7.2 微量元素： FeSO4.7H2O 0.1g MnCl2.4H2O 0.1g ZnSO4.7H2O 0.1g 93. Oatmeal Agar 瓊脂 5.0g 蒸餾水500ml 加熱溶化速溶燕麥粉40.0g 蒸餾水250ml 加熱溶化后與瓊脂液混合加水至1000ml pH 5.8 94. V8-Juice Agar (AYCC 343) V-8 果汁200g CaCO3 3.0g 瓊脂 20.0g 蒸餾水1000ml pH 5.8 95. Czapek-Dox Agar (ATCC 312) NaNO3 3.0g K2HPO4 1.0g MgSO4.7H2O 0.5g KCl 0.5g FeSO4.7H2O 0.01g 蔗糖(單獨滅菌) 30.0g 瓊脂 20.0g 蒸餾水1000ml pH 5.6 A 液 瓊脂 5.0g GC瓊脂培養(yǎng)基(BBL 11275) 36.0g 蒸餾水500.0ml B 液 干燥色牛血紅蛋白(BBL 11871) 10.0g 蒸餾水500.0ml Iso Vitale X (BBL 11876) 10.0ml 97. 固氮螺菌培養(yǎng)基(ATCC 838) K2HPO4 0.1g KH2PO4 0.4g MgSO4.7H2O 0.2g NaCl 0.1g CaCl2 0.02g FeCl3 0.01g NaMoO4.2H2O 0.002g 蘋果酸鈉(Na malate) 5.0g 酵母膏0.05g 蒸餾水1000ml pH 7.2-7.4 98. BG-11 NaNO3 1.5g K2HPO4 0.04g MgSO4.7H2O 0.075g CaCl2.7H2O 0.036g Na2CO3 0.02g 檸檬酸0.006g 檸檬酸鐵0.006g *微量元素溶液A5 1ml 氨芐青霉素 (終濃度) 50μg/ml 蒸餾水1000ml 瓊脂 20.0g * 微量元素溶液A5 H3BO4 2.86g MnCl2.4H2O 1.81g ZnSO4 0.222g Na2MoO4 0.39g CuSO4.5H2O 0.079g Co(NO3)2.6H2O 49.4g 99. (Marine Agar) 蛋白胨5.0g 酵母膏1.0g 檸檬酸鐵0.1g NaCl 19.45g MgSO4.7H2O 5.9g KCl 0.55g Na2SO4 3.24g CaCl2 1.8g NaHCO3 0.16g KI 0.08g 微量元素10ml pH 7.0-7.6 * 微量元素溶液： 氯化鍶0.034g 硅酸鈉0.004g 蒸餾水1000ml H3BO4 0.022g NaCl 0.0024g NaNO3 0.0016g NaHPO4 0.008g 蒸餾水1000ml 100.(Yeast Starch Agar) 酵母膏2.0g 可溶性淀粉10.0g 瓊脂 20.0g 蒸餾水1000ml pH 7.2-7.4 101.GYMC 葡萄糖4.0g 酵母膏2.0g 麥芽汁20.0g 瓊脂20.0g CaCO3 1.5g 蒸餾水1000ml pH 6.8-7.0 102. LCSB 乳糖15.0g 玉米漿5.0g 蛋白胨5.0g NaCl 4.0g MgSO4.7H2O 0.5g KH2PO4 0.6g FeCl3.6H2O 0.005g CuSO4.5H2O 0.002g 瓊脂 20.0g 蒸餾水1000ml 相對濕度65% pH 4.8 103.高氏1號(G) 可溶性淀粉20.0g KNO3 1.0g K2HPO4 0.5g MgSO4.7H2O 0.5g NaCl 0.5g FeSO4.7H2O 0.01g 瓊脂 20.0g 蒸餾水1000ml pH 7.0-7.2 104.葡萄糖-酵母膏瓊脂(ATCC 846) 酵母膏5.0g 蛋白胨1.0g KH2SO4 1.0g NaCl 1.0g 瓊脂 20.0g 蒸餾水950.0ml pH 6.8 補加50ml10%葡萄糖(葡萄糖溶液單獨過濾滅菌)。 105.Tep尿液(Uric Acid)瓊脂(ATCC 894) Na2HPO4.12H2O 9.0g KH2PO4 1.5g MgSO4.7H2O 0.2g 檸檬酸鐵銨(綠色) 1.2g CaCl2 20.0mg MnCl2.4H2O 1.0mg Trypticcase Soy Broth (BBL 11768) 3.0g 尿素4.0g 瓊脂 20.0 蒸餾水1000ml pH 7.2 106.增強梭菌生長的培養(yǎng)基(ATCC 1053) 胰蛋白胨10.0g 牛肉膏10.0g 酵母膏3.0g 葡萄糖5.0g NaCl 5.0g 可溶性淀粉1.0g 半胱氨酸鹽酸鹽0.5g 醋酸鈉3.0g 瓊脂 15.0g 蒸餾水1000ml pH 6.6-7.0 107.酵母膏-甘露(糖)醇瓊脂(ATCC 1205) K2HPO4 0.5g MgSO4.7H2O 0.2g NaCl 0.1g 甘露(糖)醇10.0g 酵母膏0.4g 蒸餾水加至1000ml 108.Tomato Dextrin Agar (ATCC 965) 番茄汁20.0g 糊精20.0g 酵母膏10.0g CoCl2.6H2O 5.0mg 瓊脂 20.0g 蒸餾水1000ml pH 7.2-7.4 109.嗜熱菌無機鹽溶液(ATCC 1554) FeSO4 1.0mg MgSO4.7H2O 200.0mg Na2HPO4 210.0mg NaH2PO4 90.0mg KCl 40.0mg CaCl2 15.0mg NaNO3 0.25g NH4Cl 0.25g 微量元素溶液(見下面) 10.0ml n-Heptadecane(C17) 0.1%(v/v) 蒸餾水1000ml 微量元素溶液： CuSO4.5H2O 500mcg H3BO4 1.0mg MnSO4.5H2O 7.0mcg ZnSO4.7H2O 7.0mg MoO3 1.0mg CoSO4.7H2O 18.0mcg 蒸餾水1000ml 110.醋桿菌培養(yǎng)基(AS 1) 葡萄糖100.0g 酵母膏10.0g CaCO3 20.0g 瓊脂15.0g 蒸餾水1000ml pH 6.8 適用范圍：園弧青霉、少根根霉、日本根霉、爪哇根霉 |
5樓2010-04-22 13:45:16
★ ★ ★
lstt09nk(金幣+3):太長了，看完后眼有點暈~~ 2010-04-22 18:25
lstt09nk(金幣+3):太長了，看完后眼有點暈~~ 2010-04-22 18:25
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29. Glucose Yeast Extract Starch Agar (???????????????????) Glucose (??????) 10g Yeast extract (?????) 10g Starch (????) 10g NaCl 5g CaCO3 3g Agar (???) 20g Distilled water (?????) 1L pH 7.0 30. Wheat Bran Medium (?????????) Wheat bran (???) 36g (NH4)2HPO4 10g K2HPO4 0.2g MgSO4??7H2O 0.1g Agar (???) 20g Distilled water (?????) 1L pH 7.0 31. Yeast Sporulation Medium (???????????????????????) (1) Kleyn Medium KH2PO4 0.012% K2HPO4 0.02% Sodium acetate (??????) 0.5% Glucose (??????) 0.062% NaCl 0.062% Peptone (??????) 0.25% Biotin (??????) 2??g% Agar (???) 2% Trace element solution (???????) 1ml/100ml [Note]: Trace element solution (??????????)?? MgSO4.7H2O 0.4%, NaCl 0.4%, CuSO4.5H2O 0.002%, MnSO4.4H2O 0.2%, FeSO4.4H2O 0.2% (2) Gorodkowa Medium Glucose (??????) 0.1% Peptone (??????) 1% NaCl 0.5% Agar (???) 2% (3) McClary Medium Glucose (??????) 0.1% KCl 0.18% Yeast juice (????) 0.25% Sodium actate (??????) 0.82% Agar (???) 1.5% 32. BPY Medium (BPY ??????) Beef extract (????) 5g Prptone (??????) 10g NaCl 5g Yeast extract (?????) 5g Glucose (??????) 5g Agar (???) 1-2% Distilled (?????) 1000ml pH 7.0 ???÷?Χ?????????(????)???????????? 33. LB Medium (LB ??????) Yeast extact (?????) 5g Peptone (??????) 10g NaCl 10g Agar (???) 1-2% Distilled water (?????) 1000ml pH 7.0 ???÷?Χ?????????(????) 34. Penssay Broth (Penssay ????) Beef extract (????) 1.5g Yeast extract (?????) 1.5g Tryptone (???????) 5g NaCl 3.5g Glucose (??????) 1g K2HPO4.3H2O 4.8g Distilled water (?????) 1000ml pH 7.2 35. Blond Agar (??????????) Peptone (??????) 10g Glucose (??????) 2g NaCl 5g NaH2PO4 2.5g Sodium citrate (????????) 5g Brain infusion (????) 800ml Pig heart infusion (?????) 200ml Sleep Blood (???) 10% pH 7.2 [Note]??Preparation of pig brain and pig heart infusion?? (1) Smash up 100g fresh pig heart (without fat and blood vessel).Add distilled water up to 1000ml; (2) Cut 200g fresh pig brain (without blood vessel). Add distilled water up to 1000ml; (3) Keep the two infusions at 50?? for 1 hr , then boil for 15 minutes; (4) Cool and filtrate through gauze . [?]???????????????(1)????????????????????????100??????????1000??????(2) ???????????????200 ??????????1000??????(3)50?汣??1С????????15?????(4)????????????????á? 36. Cellulose??decomposing Bacteria Synthetic Medium (???????????????) NaCl 6g MgSO4.7H2O 0.1g KH2PO4 0.5g CaCl2 0.1g K2HPO4 2.0g Yeast extract (?????) 1.0g (NH4)2SO4 2.0g Distilled water (?????) 1000ml Filter paper strip: one strip (?????????) pH 7.0-7.4 [Note]: So;id medium for cellulose-decomposing bacteria: Carboxymethyl Cellulose 2% Agar 2%. (б????????????????2%????? 2%???????????CMCNa ) 37. Clostridium Pasreurianum Synthetic Medium (?????????????????????) Glucose (??????) 1% MnSO4 0.001% KH2PO4 0.05% FeSO4 0.001% K2HPO4 0.05% Yeast extract(?????) 0.1% MgSO4.7H2O 0.02% Peptone (??????) 0.01% NaCl 0.001% CaCO3 0.5% Distille water (?????) 100% pH 7.0 38. Yeast Extract-Medium Extract Agar ?? (???????????????) Yeast extract (?????) 4g Mall extract (?????) 10g Glucose (??????) 4g Agar (???) 17g Distilled water (????) 1000ml pH 7.3 39. Oatemeal Agar (????????) Oatemeal(infusion)[???????] 20g Agar (???) 18% Trace element solution (?????????)1ml Distilled water up to (?????????????1000 ????) 1000ml pH 7.2 [Note]??Trace element solution (?????????)?? FeSO4.7H2O 0.1g MnCl2.4H2O 0.1g ZnSO4.7H2O 0.1g Distilled water (?????) 100ml40. Calcium Malate Agar (?????????) Calium malate (??????) 10g K2HPO4 0.5g NH4Cl 0.5g Glycerol (????) 10g Agar (???) 15g Distilled water (?????) 1000ml |
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