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07shenhan鐵蟲 (初入文壇)
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[交流]
【求助/交流】有熒光標(biāo)記的多肽在進(jìn)行低聚物處理后熒光淬滅？
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實(shí)驗(yàn)步驟如下，是參考國外文獻(xiàn)的： •Solid A peptide was dissolved in cold hexafluoro-2-propanol (HFIP). •The peptide was incubated at room temperature for at least 1 h to establish monomerization and randomization of structure. •The HFIP was removed by evaporation, and the resulting peptide was stored as a film at -20 or -80 °C. •The resulting film was dissolved in anhydrous DMSO at 5 mM and then diluted into the appropriate concentration and buffer with vortexing. •Next, the solution was aged 24 h at 4-8 °C. The sample was then centrifuged at 14000g for 10 min at 4-8 °C; the soluble oligomers were in the supernatant. •Usually, a small pellet was observed suggesting there was some pelletable material in 24 h aggregations. 我照著做了，但是溶入F12以后就形成了絮狀物（紅色的，因?yàn)闃?biāo)記了熒光）。但是放置冰箱的過程中，紅色就不見了，絮狀物也不見了。為什么呀？離心完就只剩下一點(diǎn)點(diǎn)顏色很淺的沉淀物了。 大家?guī)兔Τ龀鲋饕獍�，謝謝啦 |
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