 |
 |
 |
| 24小時(shí)熱門(mén)版塊排行榜 |
- >論壇更新日志 (921)
- >考研 (89)
- >導(dǎo)師招生 (53)
- >蟲(chóng)友互識(shí) (49)
- >基金申請(qǐng) (25)
- >休閑灌水 (14)
- >碩博家園 (9)
- >經(jīng)濟(jì)學(xué) (6)
- >博后之家 (6)
- >文獻(xiàn)求助 (6)
- >教師之家 (5)
- >微米和納米 (4)
- >考博 (4)
- >論文投稿 (4)
- >找工作 (3)
- >公派出國(guó) (2)
 |
 |
 |
||
 |
 |
 |
 |
 |
| 5 | 1/1 | 返回列表 |
| 查看: 593 | 回復(fù): 4 | |||
| 當(dāng)前只顯示滿(mǎn)足指定條件的回帖，點(diǎn)擊這里查看本話題的所有回帖 | |||
chuang1381新蟲(chóng) (初入文壇)
|
[交流]
求助英譯漢
|
||
|
Cloning and sequencing of chicken miRNAs. Secondary CEFs, prepared by routine techniques, were infected with the RB1B strain of MDV for 24 h at a multiplicity of infection of 25,000 PFU/106 cells. Protocols developed previously in one of our laboratories were used to construct the libraries (49). Briefly, RNA was isolated using TRIzol and size fractionated using polyethylene glycol (PEG) precipitation. The low-molecular-weight fraction was electrophoresed ona 15% polyacrylamide–8 M urea gel, and small RNAs (20 to 27 nt) were extracted from the gel and purified. Both 5 and 3 RNA adapters (Dharmacon Research, Boulder, CO) were sequentially ligated onto the RNA using T4 RNA ligase (Ambion, Austin, TX). The 5 RNA adapter (5-OH-GGUCUUAGUCG CAUCCUGUAGAUGGAUC-OH 3) and 3 RNA adapter (5-P-CACUGAU GCUGACACCUGC-idT-3; idT is inverted deoxythymidine) were designed to prevent self-ligation, and the ligation products were purified following each step. The RNA was then reverse transcribed (Superscript reverse transcriptase [RT]; Invitrogen) using a primer complementary to the 3 adapter. cDNA inserts were amplified by PCR using primers corresponding to both adapters. Amplicons were sequenced by using the 454 Life Sciences system that utilizes pyrosequencingbased, sequence-by-synthesis, high-throughput, parallel sequencing (52). Sequence data were filtered for adapter sequences and clustered (allowing a 4-base overhang or mismatch at either end), and the insert sequence was analyzed by comparing it to the chicken and MDV genomes and to the miRNA database (55) using BLAST (3). |
回復(fù)此樓» 猜你喜歡
本人考085602 化學(xué)工程 專(zhuān)碩
已經(jīng)有23人回復(fù)
-
求調(diào)劑院校信息
已經(jīng)有4人回復(fù)
-
085600材料與化工306
已經(jīng)有4人回復(fù)
-
286求調(diào)劑
已經(jīng)有10人回復(fù)
-
328求調(diào)劑，英語(yǔ)六級(jí)551，有科研經(jīng)歷
已經(jīng)有9人回復(fù)
-
一志愿北京化工大學(xué)070300 學(xué)碩336求調(diào)劑
已經(jīng)有4人回復(fù)
-
286分人工智能專(zhuān)業(yè)請(qǐng)求調(diào)劑愿意跨考！
已經(jīng)有8人回復(fù)
-
資源與環(huán)境 調(diào)劑申請(qǐng)(333分)
已經(jīng)有5人回復(fù)
-
280求調(diào)劑
已經(jīng)有12人回復(fù)
-
269專(zhuān)碩求調(diào)劑
已經(jīng)有5人回復(fù)
chuang1381
新蟲(chóng) (初入文壇)
- 應(yīng)助: 0 (幼兒園)
- 金幣: 143
- 帖子: 11
- 在線: 1.2小時(shí)
- 蟲(chóng)號(hào): 830865
- 注冊(cè): 2009-08-19
|
MDV是馬立克病毒，CEFs是雞胚成纖維細(xì)胞，PCR是一種擴(kuò)增技術(shù)，你好像不是學(xué)分子生物學(xué)的，你可能不太了解這個(gè)內(nèi)容，我怕你翻譯不了，但是我還是很高興你能回我貼，交個(gè)朋友很好，Marek’s Disease Virus Encodes MicroRNAs That Map to meq and the Latency-Associated Transcript是這個(gè)文章的題目，你也可以去NCBI下到，我說(shuō)得只是方法中的一部分，我對(duì)這個(gè)方法不太了解，所以我想找人了解一下這個(gè)技術(shù) |
4樓2010-04-28 09:23:09
2樓2010-04-27 20:33:39
chuang1381
新蟲(chóng) (初入文壇)
- 應(yīng)助: 0 (幼兒園)
- 金幣: 143
- 帖子: 11
- 在線: 1.2小時(shí)
- 蟲(chóng)號(hào): 830865
- 注冊(cè): 2009-08-19
3樓2010-04-28 09:15:02
5樓2010-05-08 10:46:45
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 化學(xué)調(diào)劑 +5 | yzysaa 2026-03-21 | 5/250 |
|
|---|---|---|---|---|
|
|
[考研] 材料工程專(zhuān)碩 348分求調(diào)劑 +3 | 冬辭. 2026-03-17 | 5/250 |
|
|
[考研] 298求調(diào)劑 +4 | 上岸6666@ 2026-03-20 | 4/200 |
|
|
|
[考研] 26考研一志愿中國(guó)石油大學(xué)(華東)305分求調(diào)劑 +6 | 嘉年新程 2026-03-15 | 6/300 |
|
|
|
[考研] 材料與化工（0856）304求 B區(qū) 調(diào)劑 +3 | 邱gl 2026-03-21 | 3/150 |
|
|
|
[考研] 22 350 本科985求調(diào)劑，求老登收留 +3 | 李軼男003 2026-03-20 | 3/150 |
|
|
|
[考研] 307求調(diào)劑 +10 | 冷笙123 2026-03-17 | 10/500 |
|
|
|
[考研] 304求調(diào)劑 +6 | 曼殊2266 2026-03-18 | 6/300 |
|
|
|
[考研] 321求調(diào)劑 +9 | 何潤(rùn)采123 2026-03-18 | 11/550 |
|
|
|
[考研] 323求調(diào)劑 +3 | 洼小桶 2026-03-18 | 3/150 |
|
|
|
[考研]
 材料與化工 322求調(diào)劑
+4
|
然11 2026-03-19 | 4/200 |
|
|
|
[考研] 350求調(diào)劑 +5 | weudhdk 2026-03-19 | 5/250 |
|
|
|
[考研] 289求調(diào)劑 +6 | 懷瑾握瑜l 2026-03-20 | 6/300 |
|
|
|
[考研] 一志愿西安交通大學(xué) 學(xué)碩 354求調(diào)劑211或者雙一流 +3 | 我想要讀研究生 2026-03-20 | 3/150 |
|
|
|
[考研] 081700化工學(xué)碩調(diào)劑 +3 | 【1】 2026-03-16 | 3/150 |
|
|
|
[考研] 材料與化工求調(diào)劑 +7 | 為學(xué)666 2026-03-16 | 7/350 |
|
|
|
[考研] 材料考研調(diào)劑 +3 | xwt。 2026-03-19 | 3/150 |
|
|
|
[考研] 0703化學(xué)336分求調(diào)劑 +6 | zbzihdhd 2026-03-15 | 7/350 |
|
|
|
[考研] 308求調(diào)劑 +4 | 是Lupa啊 2026-03-16 | 4/200 |
|
|
|
[考研] 一志愿蘇州大學(xué)材料工程（085601）專(zhuān)碩有科研經(jīng)歷三項(xiàng)國(guó)獎(jiǎng)兩個(gè)實(shí)用型專(zhuān)利一項(xiàng)省級(jí)立項(xiàng) +6 | 大火山小火山 2026-03-16 | 8/400 |
|