 |
 |
 |
 |
 |
 |
||
 |
 |
 |
 |
 |
| 2 | 1/1 | 返回列表 |
| 查看: 1265 | 回復(fù): 1 | |||
| 本帖產(chǎn)生 1 個 翻譯EPI ，點擊這里進行查看 | |||
hanyan2004銀蟲 (初入文壇)
|
[交流]
胰島素增敏活性篩選實驗操作翻譯
|
||
|
胰島素增敏活性篩選實驗 用含10% FBS的高糖有酚紅DMEM 培養(yǎng)基在六孔板中培養(yǎng)3T3-L1細胞，至匯合度達90%進行誘導(dǎo)分化，用含10% FBS，Ins, Dex, IBMX 的高糖有酚紅DMEM 培養(yǎng)基誘導(dǎo)，四天后重新消化接種于24孔板，用含10% FBS的高糖有酚紅DMEM 培養(yǎng)基培養(yǎng)兩天。然后再用含10% FBS和較低濃度Ins較高濃度 Dex的高糖有酚紅DMEM 培養(yǎng)基進行誘導(dǎo)胰島素抵抗，四天后換用含待測樣品的高糖無酚紅DMEM培養(yǎng)基，同時設(shè)立空白對照(誘導(dǎo)分化后的正常細胞)和陰性對照(誘導(dǎo)分化后的胰島素抗性細胞)不加樣品。繼續(xù)培養(yǎng)50h后取10ul 培養(yǎng)上清, 轉(zhuǎn)到酶標板中，采用葡萄糖檢測試劑盒檢測葡萄糖水平，490nM測定OD值。實驗時，每個樣品設(shè)雙復(fù)孔，重復(fù)兩次。根據(jù)OD值計算增敏率，增敏率=[1-(OD樣品-OD空白)/(OD陰性-OD空白)] ×100%，相對增敏率=樣品增敏率/羅格列酮增敏率。 |
回復(fù)此樓» 猜你喜歡
289求調(diào)劑
已經(jīng)有3人回復(fù)
-
317求調(diào)劑
已經(jīng)有10人回復(fù)
-
廣西大學(xué)家禽遺傳育種課題組2026年碩士招生（接收計算機專業(yè)調(diào)劑）
已經(jīng)有3人回復(fù)
-
學(xué)校已經(jīng)提交到NSFC，還能修改嗎？
已經(jīng)有8人回復(fù)
-
08工學(xué)調(diào)劑
已經(jīng)有5人回復(fù)
-
281求調(diào)劑（0805）
已經(jīng)有25人回復(fù)
-
085600材料與化工
已經(jīng)有6人回復(fù)
-
265求調(diào)劑
已經(jīng)有8人回復(fù)
-
0817 化學(xué)工程 299分求調(diào)劑 有科研經(jīng)歷 有二區(qū)文章
已經(jīng)有21人回復(fù)
-
295材料求調(diào)劑，一志愿武漢理工085601專碩
已經(jīng)有4人回復(fù)
jingjing1005
木蟲 (小有名氣)
- 翻譯EPI: 27
- 應(yīng)助: 0 (幼兒園)
- 金幣: 1556.9
- 紅花: 1
- 帖子: 191
- 在線: 91.8小時
- 蟲號: 1022144
- 注冊: 2010-05-19
- 專業(yè): 質(zhì)譜分析
★
wypward(金幣+1):歡迎新蟲，辛苦啦 2010-06-01 10:51:07
hanyan2004(金幣+50, 翻譯EPI+1): 2010-06-01 23:17:06
hanyan2004(金幣+1): 2010-06-02 12:10:53
hanyan2004(金幣+1):翻譯得很好，謝謝你哈 2010-06-02 21:37:14
wypward(金幣+1):歡迎新蟲，辛苦啦 2010-06-01 10:51:07
hanyan2004(金幣+50, 翻譯EPI+1): 2010-06-01 23:17:06
hanyan2004(金幣+1): 2010-06-02 12:10:53
hanyan2004(金幣+1):翻譯得很好，謝謝你哈 2010-06-02 21:37:14
|
Screening test of Insulin-sensitizing activity 3T3-L1 cells were cultured in high glucose DMEM culture meduim with phenol red Containing 10% FBS in six-well plates. When confluence reached 90%, cells were induced to differentiate with a high sugar DMEM culture medium with phenol red containing 10% FBS, Ins, Dex, IBMX. After four days, cells were seeded in 24 well plates after re-digestion, cultured in high glucose DMEM culture medium with phenol red containing 10% FBS for two days. and then they were induced for insulin resistance using high glucose DMEM culture medium with phenol red containing 10% FBS, Ins in lower concentration and Dex in higher concentration. Four days later the cells were transferred to the high glucose DMEM medium without phenol red containing sample, while the control test (normal cells after induction of differentiation) and negative control test (insulin resistant cells after induced differentiation without sample) were established. 10ul supernatants were taken to ELISA plate after 50hs’ continuous culture to test the glucose level using glucose testing kit and OD values in 490nm. During the experiment, a double recovery was set for each sample hole and the same experiment was repeated twice. The increased sensitivity rate was calculated according to OD values, increased sensitivity rate = [1 - (OD sample-OD blank) / (OD negative-OD blank)] × 100%, the relative increased sensitivity rate = increased sensitivity of sample / increased sensitivity of rosiglitazone. |
2樓2010-06-01 10:22:34
相關(guān)版塊跳轉(zhuǎn)
我要訂閱樓主 hanyan2004 的主題更新
| 2 | 1/1 | 返回列表 |
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 317求調(diào)劑 +5 | 申子申申 2026-03-19 | 10/500 |
|
|---|---|---|---|---|
|
[考研] 求調(diào)劑 +4 | Mqqqqqq 2026-03-19 | 4/200 |
|
|
|
[考研]
 295復(fù)試調(diào)劑
+7
|
簡木ChuFront 2026-03-19 | 7/350 |
|
|
|
[考研] 能源材料化學(xué)課題組招收碩士研究生8-10名 +5 | 脫穎而出 2026-03-16 | 14/700 |
|
|
|
[論文投稿]
申請回稿延期一個月，編輯同意了。但系統(tǒng)上的時間沒變，給編輯又寫郵件了，沒回復(fù)
 10+3
|
wangf9518 2026-03-17 | 4/200 |
|
|
|
[考研] 266求調(diào)劑 +5 | 陽陽哇塞 2026-03-14 | 10/500 |
|
|
|
[考研] 286求調(diào)劑 +6 | lemonzzn 2026-03-16 | 10/500 |
|
|
|
[考研] 328求調(diào)劑，英語六級551，有科研經(jīng)歷 +4 | 生物工程調(diào)劑 2026-03-16 | 12/600 |
|
|
|
[考研] 一志愿天大材料與化工（085600）總分338 +5 | 蔡大美女 2026-03-13 | 5/250 |
|
|
|
[考研] 0703化學(xué)調(diào)劑，求各位老師收留 +10 | 秋有木北 2026-03-14 | 10/500 |
|
|
|
[考研] 材料工程專碩調(diào)劑 +5 | 204818@lcx 2026-03-17 | 6/300 |
|
|
|
[考研] 0854可跨調(diào)劑，一作一項核心論文五項專利，省、國級證書40+數(shù)一英一287 +8 | 小李0854 2026-03-16 | 8/400 |
|
|
|
[考研] 301求調(diào)劑 +4 | A_JiXing 2026-03-16 | 4/200 |
|
|
|
[論文投稿] 有沒有大佬發(fā)小論文能帶我個二作 +3 | 增銳漏人 2026-03-17 | 4/200 |
|
|
|
[考研]
 11408 一志愿西電，277分求調(diào)劑
+3
|
zhouzhen654 2026-03-16 | 3/150 |
|
|
|
[考研] [導(dǎo)師推薦]西南科技大學(xué)國防/材料導(dǎo)師推薦 +3 | 尖角小荷 2026-03-16 | 6/300 |
|
|
|
[考研] 304求調(diào)劑 +4 | ahbd 2026-03-14 | 4/200 |
|
|
|
[考研] 070300化學(xué)學(xué)碩求調(diào)劑 +6 | 太想進步了0608 2026-03-16 | 6/300 |
|
|
|
[考研] 0703化學(xué)調(diào)劑 290分有科研經(jīng)歷，論文在投 +7 | 膩膩gk 2026-03-14 | 7/350 |
|
|
|
[考研] 289求調(diào)劑 +4 | 這么名字咋樣 2026-03-14 | 6/300 |
|