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【求助】有人做量子點(diǎn)顯微鏡成像的嗎? 已有5人參與
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顯微鏡成像的時(shí)候有沒有發(fā)現(xiàn)量子點(diǎn)是一開始激發(fā)時(shí)不亮,但是激發(fā)1分鐘后慢慢變得很亮?這種問題怎么解決? 你們做量子點(diǎn)的時(shí)候有沒有發(fā)現(xiàn)量子點(diǎn)會越照越亮? |
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我做的是普通寬場熒光顯微鏡,我?guī)熜肿龅氖羌す夤簿劢梗覀儌z都觀察到這個(gè)現(xiàn)象了。 我問了一個(gè)老外,在science上發(fā)過一篇量子點(diǎn)在活細(xì)胞成像的review。他的回答如下: this is not a new phenomenon and has been observed at the very beginning of QD use in biology (more than 10 years ago). The idea is that some of the QDs are dark (this is why the quantum yield (QY) is usually much less than 1, or at least becomes so in real life situations). This is because of surface defects, imperfect coating, etc... However, cells contain a lot of molecules (as you can guess), among which small cystein-rich or thiolated molecules. This group (-SH) is known to passivate QD's surface very well, increasing the QY and in some case suppressing the blinking of individual QDs. The longer the QDs are in cells. the greater this passivation... This is what you are observing. Unfortunately, short of having perfectly passivated QDs from the get-go, you will always observe this in live cells... 他說是表面缺陷導(dǎo)致不亮,但是很多地方都說就是因?yàn)楸砻嫒毕輰?dǎo)致量子點(diǎn)發(fā)光的。 到底是怎么回事?表面缺陷到底是引起量子點(diǎn)不亮還是發(fā)亮的原因? |
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