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yanghong206銀蟲 (小有名氣)
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1.實驗所選各種蛋白酶最適反應(yīng)條件 2.DPPH•試驗加樣表 3.五種酶的酶活力 4.分別經(jīng)過Protamex、Neutrase、Alcalase、酸性蛋白酶和Papain酶解的5種不同的鯊魚皮明膠水解產(chǎn)物對DPPH•自由基、ABTS自由基以及對Fe2+誘導(dǎo)卵黃脂蛋白多不飽和脂肪酸的過氧化反應(yīng)的清除作用 5.明膠水解產(chǎn)物的SP-Sephadex C-25離子交換色譜 6.陽離子交換色譜純化組分的DPPH•自由基清除活性 7.P2的Sephadex G-50凝膠過濾層析色譜 8.凝膠過濾色譜洗脫組分的DPPH自由基•清除活性 9.明膠水解產(chǎn)物及洗脫峰M2的小分子電泳圖 電泳條件：工作電壓：濃縮膠，中間膠40V，分離膠60V 10.標準樣品電泳遷移率圖 |
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yanghong206(金幣+10, 翻譯EPI+1): 2010-07-31 08:40:10
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1.實驗所選各種蛋白酶最適反應(yīng)條件The optimal reaction conditions of various proteases by experiments 2.DPPH•試驗加樣表 DPPH•experiment sample table 3.五種酶的酶活力 activities of five enzymes 4.分別經(jīng)過Protamex、Neutrase、Alcalase、酸性蛋白酶和Papain酶解的5種不同的鯊魚皮明膠水解產(chǎn)物對DPPH•自由基、ABTS自由基以及對Fe2+誘導(dǎo)卵黃脂蛋白多不飽和脂肪酸的過氧化反應(yīng)的清除作用 the ability of shark skin gelatin hydrolysis products catalysed by five different enzymes Protamex, Neutrase, Alcalase, acidic protease, and Papain to eliminate DPPH•radicals and ABTS radicals as well we inhibit Fe(II)-induced yolk lipoprotein polyunsaturated fatty acid peroxidation. 5.明膠水解產(chǎn)物的SP-Sephadex C-25離子交換色譜 the SP-Sephadex C-25 cation exchange chromatography of gelatin hydrolysis products 6.陽離子交換色譜純化組分的DPPH•自由基清除活性 the activity to eliminate DPPH•radicals of components purified by cation exchange chromatography 7.P2的Sephadex G-50凝膠過濾層析色譜 the Sephadex G-50 gel filtration chromatography of P2 8.凝膠過濾色譜洗脫組分的DPPH自由基•清除活性 the activity to eliminate DPPH•radicals of components eluted by gel filtration chromatography 9.明膠水解產(chǎn)物及洗脫峰M2的小分子電泳圖 the electrophoresis of hydrolysis products of gelatin and elution peak M2 電泳條件：工作電壓：濃縮膠，中間膠40V，分離膠60V electrophoresis conditions: Operating Voltage: 40V for stacking and intermediate gel 60V for separating gel 10.標準樣品電泳遷移率圖 the electrophoretic mobility plan of standard sample |
2樓2010-07-28 22:11:33
yanghong206(金幣+10): 2010-07-31 08:40:16
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有些小改動: 1.實驗所選各種蛋白酶最適反應(yīng)條件The optimal reaction conditions of various proteases by experiments 2.DPPH•試驗加樣表 DPPH•experiment sample table 3.五種酶的酶活力 activities of five enzymes 4.分別經(jīng)過Protamex、Neutrase、Alcalase、酸性蛋白酶和Papain酶解的5種不同的鯊魚皮明膠水解產(chǎn)物對DPPH•自由基、ABTS自由基以及對Fe2+誘導(dǎo)卵黃脂蛋白多不飽和脂肪酸的過氧化反應(yīng)的清除作用 the ability of shark skin gelatin hydrolysis products catalysed by five different enzymes Protamex, Neutrase, Alcalase, acidic protease, and Papain to eliminate DPPH•radicals and ABTS radicals and inhibit Fe(II)-induced yolk lipoprotein polyunsaturated fatty acid peroxidation. 5.明膠水解產(chǎn)物的SP-Sephadex C-25離子交換色譜 the SP-Sephadex C-25 cation exchange chromatography of gelatin hydrolysis products 6.陽離子交換色譜純化組分的DPPH•自由基清除活性 the activity to eliminate DPPH•radicals of components purified by cation exchange chromatography 7.P2的Sephadex G-50凝膠過濾層析色譜 the Sephadex G-50 gel filtration chromatography of P2 8.凝膠過濾色譜洗脫組分的DPPH自由基•清除活性 the activity to eliminate DPPH•radicals of components eluted by gel filtration chromatography 9.明膠水解產(chǎn)物及洗脫峰M2的小分子電泳圖 the electrophorogram of gelatin hydrolysis products and elution peak M2 電泳條件：工作電壓：濃縮膠，中間膠40V，分離膠60V electrophoresis conditions: Operating Voltage: 40V for stacking and intermediate gel 60V for separating gel 10.標準樣品電泳遷移率圖 the electrophoretic mobility plan of standard sample |
3樓2010-07-28 22:18:03
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