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本研究以實(shí)驗(yàn)室已構(gòu)建的帶有β-半乳糖苷酶(LacZ)報告基因并缺失MDV雙拷貝Meq基因的四川省眉山毒株(rMS△Meq)為親本病毒,通過同源重組的方法構(gòu)建了缺失mdv-miR-M9、-M5、-M12的四川省眉山地區(qū)的MDV ,命名為rMS△miR9-12,用PCR方法和測序方法進(jìn)行鑒定,證明此病毒的mdv1-miR-M9至mdv1-miR-M12編碼的基因已缺失,并將親本病毒缺失的Meq基因已補(bǔ)齊。 因?yàn)槭钦,盡量使用被動語態(tài),不要出現(xiàn)we等詞 |
新蟲 (正式寫手)
| The study on the laboratory construction with beta - galactoside enzyme LacZ report gene and the lack of Sichuan Meishan MDV two copies of Meq gene strains of RMS Meq parent viruses, by Homologous Recombination method for constructing the lack of mdv-miR-M9, -M5, -M12 MDV in Meishan of Sichuan province, named RMS miR9-12, with PCR methods and sequence identification methods to prove that this mdv1-miR-M9 to mdv1-miR-M12 coding genes of the virus has been missing and the lack of parental virus Meq gene fill in. |
This research laboratories have to build a β-lacz report and missing genetic copies of the gene pair meq mdv meishan, sichuan province of the rms △meq Qinben virus for the necessary restructuring of the way to build the missing mdv - - and - mir m9 m5 m12 meishan, and provincial areas of the rms mdv, named △ mir9 -12, pcr method and order means that the identification of the virus mir mdv1 - - - - mdv1 m9 to the mir m12 of genes is missing and Qinben virus meq genes are the missing piece.![]() Ps:偶不懂什么叫回貼應(yīng)助,所以就填寫了這個~ 偶在練習(xí)英語,所以。。 不好意思的哈 ![]() [ Last edited by 小小如 on 2010-8-4 at 18:00 ] |
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