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CCZ2008銀蟲 (小有名氣)
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[交流]
———————— 關(guān)于LB瓊脂的疑問
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試驗室預(yù)購LB瓊脂,想選Amresco的,但Amresco的LB Agar分兩種,分別是 LB Agar, Lennox Formulation LB Agar, Miller Formulation 請問這兩種的區(qū)別是什么?應(yīng)用范圍? |
榮譽版主 (知名作家)
CA-FANS
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配方不一樣,后者的氯化鈉含量比較高 LB Broth, Miller contains twenty times the sodium chloride level of Luria Broth Base, Miller and twice the level found in LB Broth, Lennox.3,5,6 This allows the researcher to select the optimal salt concentration for a specific strain in LB Agar, Miller. https://www.bd.com/ds/technicalCenter/inserts/LB_Agar_Lennox.pdf https://www.bd.com/ds/technicalCenter/inserts/LB_Agar_Miller.pdf Lennox Formulation Approximate Formula* Per Liter Tryptone ...................................................................10.0 g Yeast Extract .............................................................. 5.0 g Sodium Chloride ........................................................ 5.0 g Agar .........................................................................15.0 g —————————————————————————————— Difco™ LB Agar, Miller Approximate Formula* Per Liter Tryptone ...................................................................10.0 g Yeast Extract .............................................................. 5.0 g Sodium Chloride ...................................................... 10.0 g Agar .........................................................................15.0 g |

榮譽版主 (知名作家)
CA-FANS

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