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強(qiáng)之寒冬金蟲 (正式寫手)
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[交流]
【求助/交流】請問MTT和MTS有什么區(qū)別! 已有7人參與
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| 以前看文獻(xiàn)只看過MTT Assay! 這兩天又看到個MTS! 我看具體名稱好像都是細(xì)胞存活率測試,請問這兩種方法有什么區(qū)別? |
金蟲 (正式寫手)
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The MTT assay and the MTS assay are colorimetric assays for measuring the activity of enzymes that reduce MTT or close dyes (XTT, MTS, WSTs) to formazan dyes, giving a purple color. A main application allows to assess the viability (cell counting) and the proliferation of cells (cell culture assays). It can also be used to determine cytotoxicity of potential medicinal agents and toxic materials, since those agents would stimulate or inhibit cell viability and growth. MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a yellow tetrazole), is reduced to purple formazan in living cells.[1] A solubilization solution (usually either dimethyl sulfoxide, an acidified ethanol solution, or a solution of the detergent sodium dodecyl sulfate in diluted hydrochloric acid) is added to dissolve the insoluble purple formazan product into a colored solution. The absorbance of this colored solution can be quantified by measuring at a certain wavelength (usually between 500 and 600 nm) by a spectrophotometer. The absorption maximum is dependent on the solvent employed.MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium), in the presence of phenazine methosulfate (PMS), produces a formazan product that has an absorbance maximum at 490-500 nm in phosphate-buffered saline.[3](維基百科粘的) MTS不過是Promega開發(fā)的一種新替代實際罷了 |

金蟲 (正式寫手)
鐵桿木蟲 (職業(yè)作家)
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