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【求助】請問inner filter effect 是什么意思 已有3人參與
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| 麻煩大家給解釋一下inner filter effect,謝謝! |
鐵桿木蟲 (著名寫手)
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Inner filter effect: includes reabsorption. Reabsorption happens because another molecule or part of a macromolecule absorbs at the wavelengths at which the fluorophore emits radiation. If this is the case, some or all of the photons emitted by the fluorophore may be absorbed again. Another inner filter effect occurs because of high concentrations of absorbing molecules, including the fluorophore. The result is that the intensity of the excitation light is not constant throughout the solution. Resultingly, only a small percentage of the excitation light reaches the fluorophores that are visible for the detection system. |
鐵桿木蟲 (職業(yè)作家)
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