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我和我自己鐵蟲 (小有名氣)
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[交流]
【求助/交流】著急，Trimmer Direct cDNA Normalization Kit有人用過嗎？ 已有1人參與
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有人用過嗎……有關(guān)于這個試劑盒的詳細介紹嗎？有人知道這個盒子的基本原理嗎?里面那個DSN是什么��？ [ Last edited by 我和我自己 on 2010-10-9 at 12:53 ] |
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小木蟲(金幣+0.5):給個紅包，謝謝回帖交流
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| cDNA normalization is essential for hunting and analyzing the rare transcripts using high-throughput approaches, such as EST sequencing projects, microarrays, functional screenings etc. The Evrogen TRIMMER-DIRECT kit is developed for normalization of cDNA enriched with full-length sequences and intended for directional cloning. While most normalization methods are not well suited for the normalization of long cDNA, TRIMMER-DIRECT allows to obtain normalized cDNA samples in which individual cDNA sizes range from 0.5 to 4.5 kb. The normalization is based on unique properties of novel Duplex Specific Nuclease (Zhulidov et al., Nucl. Acids. Res. 2004, 32: e37). It is performed prior to cDNA cloning, and does not include physical separation steps. Normalization using the TRIMMER-DIRECT kit significantly increases the efficiency of random sequencing of amplified cDNA. |
2樓2010-10-10 11:55:06
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