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Wet biomass (0.4 g) was ground in 1.2 ml of 2% (wt/vol) trichloroacetic acid (Fisher Scientific, Hampton, N.H.) in a chilled mortar and pestle with 0.2 g of ground glass beads and then centrifuged for 5 min. Polypho-sphate of different chain lengths was extracted from the supernatant using the methods of Clark et al. (10). Polyphosphate concentration was determined in the fractions as orthophosphate using the colorimetric assay of Saheki et al. (39). Prior to analysis, polyphosphate in the fractions was converted to orthophos-phate by acid hydrolysis, and orthophosphate released from polyphosphates was differentiated from orthophosphate from other sources by previously published methods (25). For ICP-OES analysis, samples were acidified with 1 ml of con-centrated nitric acid, diluted to 12 ml with MilliQ dH2O, and filtered with 0.2 m mesh syringe filters as described above. 謝謝! |
鐵桿木蟲 (正式寫手)
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在盛有1.2ml 2%(質(zhì)量/體積)三氯乙酸和0.2克杵磨玻璃珠的低溫冷凍過的研缽中(菲舍爾科技,漢普頓,新罕布什爾州)研磨潮濕的生物質(zhì)原料(0.4克),然后離心5分鐘。 靜置后,使用克拉克等人的方法從上層漂浮物中提取不同鏈長的多聚磷酸酯。(10)。多聚磷酸酯含量測定利用Saheki等比色法以正磷酸酯測算。 (39)。 基于以上分析,多聚磷酸酯測算轉(zhuǎn)化為正磷酸酯的酸解測算,從多磷酸酯中分離的正磷酸酯與之前公布的從其他來源的方法(25)中的磷酸酯是有區(qū)別的。對于ICP - OES的分析,樣品用1毫升的濃硝酸酸化,經(jīng)MilliQ dH2O稀釋至12毫升后,如上所述用0.2米網(wǎng)狀過濾器過濾 |
| 濕生物質(zhì)(0.4)是在12毫升的2%(重)trichloroacetic酸(費舍爾第N.H.科學(xué)、漢普頓,在所有臼與磨砂玻璃珠0.2克,然后判讀5分鐘。Polypho-sphate鏈的提取方法,浮克拉克等。(十)。佳潔士皓爽白牙膏濃度的測定痕量活性磷的分數(shù)為利用色度學(xué)檢測的Saheki等。(39)。在分析之前,多聚磷酸鈉的分數(shù)是由酸水解orthophos-phate轉(zhuǎn)換,并正磷酸鹽免除多是來自其他來源的區(qū)別開來,痕量活性磷由先前發(fā)表的方法(25)。為ICP-OES分析、樣品和1毫升的con-centrated acidified硝酸、稀釋至12毫升和MilliQ dH2O、過濾和0.2 m網(wǎng)格注射器過濾器如上所述。 |

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