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wensu木蟲(chóng) (正式寫(xiě)手)
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Morphological features and isoenzyme characterization of permanently isolated endosymbionts from green hydra have been undescribed until now. Morphological characteristics (cell area, cell radius, chloroplast area) and isoenzyme patterns of esterase (EST), peroxidase (POX), and catalase (CAT) of aposymbiotic algae from green hydra (Cx) and from reference species Chlorella kessleri (Ck) and Chlorella vulgaris (Cv) were compared. Changeable morphology of isolated algae determined by light and electron microscopy was noticed. Results obtained by the native anodic electrophoresis suggest complex relations within the Chlorella genus. The greatest isoenzyme diversity was noted in EST patterns of isolated endosymbionts. Due to their much higher heterogeneity, EST isoenzymes seemed to be more suitable genetic markers for identification of different Chlorella species than CAT and POX isoenzymes. Morphological characteristics of researched species confirmed the interspecific diversity noticed by isoenzyme analysis |
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wensu(金幣+17, 翻譯EPI+1):強(qiáng)人 2010-11-09 10:21:53
| 到現(xiàn)在為止，對(duì)從綠色水螅中分離出來(lái)的endosymbionts已經(jīng)進(jìn)行了形態(tài)特征研究及其同工酶的表征。對(duì)從綠色水螅中分離出來(lái)的aposymbiotic的 同工酶（酯酶，過(guò)氧化酶和過(guò)氧化氫酶）的表征及其形態(tài)特征（細(xì)胞面積、半徑、葉綠素面積）與普通小球藻、特異相關(guān)性的小球藻的比較發(fā)現(xiàn)：分離出來(lái)的藻類的形貌發(fā)生了改變（此形貌通過(guò)光學(xué)、電子顯微鏡進(jìn)行表征）。通過(guò)原位的陽(yáng)極電泳結(jié)果顯示其屬于小球藻的種屬一員。其中endosymbiont的EST類型的同工酶種類多樣性最多。由于EST類型的同工酶具有更大的異質(zhì)性特征，它比CAT\POX同工酶更能作為種屬區(qū)分的遺傳性特征。對(duì)所研究的酶的形態(tài)研究更加證實(shí)了同工酶分析中的種間差異。 |
2樓2010-11-08 21:45:18
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