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MI是由***大學(xué)在1980選取中國(guó)云南省西雙版納小耳豬品系建立的，建系的一對(duì)系祖是當(dāng)時(shí)已具有一定近交基礎(chǔ)的一頭母豬和其所生的小公豬。培育過(guò)程中一直采用全同胞或親子交兩種高度近交方式連續(xù)近交，且每一世代均進(jìn)行嚴(yán)格的選擇，由于近交系的雜合基因在近交過(guò)程中得以分離、重組和不斷純合，BMI現(xiàn)已形成具有不同表型和基因型的6個(gè)家系和18個(gè)亞系。BMI的兩個(gè)原始系祖均為黑毛色表型，隨著近交和世代進(jìn)展，后來(lái)分離出了花毛表型，即從毛色表型上看，BMI有黑毛色和花毛色。從近交父母本及后代表型看：黑毛色表型間兩兩交配生下的仔豬有全黑毛色表型和黑毛色與花毛色表型共存兩種情況；黑毛色和花毛色交配的后代也存在全黑毛色表型和黑毛色與花毛色表型共存兩種情況；花毛表型間兩兩交配生出的全是花毛色表型。因?yàn)榇蠖鄶?shù)哺乳動(dòng)物的皮毛顏色遺傳與E座位的MC1R基因緊密相關(guān)，而且黑皮毛色多為顯性，于是我們根據(jù)BMI系譜從表型推斷黑毛色對(duì)花毛色為顯性，假設(shè)為E座位一對(duì)等位基因EBMI/Ebmi控制，則黑毛色為EBMI/EBMI和EBMI/Ebmi二種基因型，花毛色為Ebmi/Ebmi基因型，為了確定是否EBMI/Ebmi真實(shí)的存在顯隱性關(guān)系，假如存在，其序列真實(shí)情況是什么？并探索其分子機(jī)理，本研究以與毛色遺傳緊密相關(guān)的存在E座位的MC1R基因作為主要候選基因，利用BMI清楚的遺傳背景和系譜圖，通過(guò)分子生物學(xué)手段對(duì)BMI的EBMI/EBMI、EBMI/Ebm和Ebmi/Ebmi基因型豬進(jìn)行MC1R基因直接測(cè)序和克隆測(cè)序來(lái)研究其毛色遺傳機(jī)理。
BMI was developed at ***University in 1980. The foundation stock were a sow and her own male piglet, which was selected from native small-ear breeds pig in Xishuangbanna, Yunnan Province, China. Then, rigorous selection and highly inbreeding methods (full-sib or parent-offspring mating) were conducted continuously generation by generation. Because heterozygotic genes were separated and recombined in the process of inbreeding, BMI now has formed six families and eighteen substrains with different phenotypes and genotypes. With the development of inbreeding and generating, the white with black spots descendants were generated although two original ancestors of BMI have the uniform black color coat phenotype. Namely, BMI were divided into the black and white with black spots phenotypes in light of the coat color . Owing to the coat color difference of the mated individuals, three cases took place. In the 1st case, uniform black color coat or black and white with black spots descendants are generated by mating of the two black phenotype pigs. In the 2nd case, the similar results to that of the above come out with mating between the black and white with black spotting pigs. In the 3th case, the descendants own the same coat color as their white with black spot parents. As it is well known, the coat color genetic process of the successor is closely related with MC1R gene in E locus. In a variety of mammals, dominant alleles at E locus act to produce a uniform black coat color, So we assume that the black phenotype be dominant to the white phenotype with black spots and the E position was controlled by a pair of EBMI/Ebmi allelic genes in BMI. The black phenotype could possess EBMI/EBMI or EBMI/Ebmi genotype, and the white with black spots phenotype associated with the Ebmi/Ebmi genotype. Up to now, it is unclear about the molecular mechanism of domination relation between EBMI and Ebmi genes. MC1R gene, one of the principal candidate genes for coat color trait, was used to analyze the coat genetic mechanism by using the clear genetic background and pedigree of BMI. The coat color genetic mechanism of BMI was investigated by molecular bio-method of the direct sequencing of PCR products and cloning sequencing of MC1R gene with the individuals of EBMI/EBMI, EBMI/Ebm and Ebmi/Ebmi genotype.

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1樓 2010-11-13 21:30:44

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huojinlong9287(金幣+100, 翻譯EPI+1): 2010-11-14 15:30:42

In 1980, BMI was exploited by ***University based on the small-ear pigs at Xishuangbanna, Yunnan Province. A pair of progenitors was a sow and her sons. Previously, the propagation was conducted by means of highly full-sib or parent-offspring inbreeding and each generations underwent the strict selection. As heterozygotic genes were separated and recombined in the process of inbreeding, BMI has already owned six families and eighteen substrains with different phenotypes and genotypes. With the development of inbreeding and generating, the white with colored descendants were generated although the progenitors of BMI were both black phenotype. Namely, BMI was divided into the black and white with mixed-color phenotypes in light of the coat color . According to the coat color difference of the mated individuals, there are three cases. In the 1st case, uniform black coat or black along with white with mixed-color descendants are generated by mating of the two black phenotype pigs. In the 2nd case, the similar results to that of the above come out with mating between the black and white with the mixed color pigs. In the 3th case, the descendants own the same coat color as their white with the mixed color parents. As it is well known, the coat color genetic process of most mammals is closely related to MC1R gene in E locus and the dominant alleles at E locus often lead to the generation of all black individuals. Therefore, it was deduced that the black phenotype was dominant as compared with the white phenotype with colored. If the E position was supposed to be controlled by a pair of EBMI/Ebmi allelic genes in BMI, the black could possess EBMI/EBMI or EBMI/Ebmi genotypes and the colored should own the Ebmi/Ebmi genotype. Up to now, it is unclear about the molecular mechanism of domination relation between EBMI and Ebmi genes. As the genetic background and pedigree of BMI is well learned, MC1R gene was used as the main candidate genes of the research for the coat genetic process and the related mechanism was investigated by molecular bio-methods, the direct sequencing of PCR products and cloning sequencing of MC1R gene with the individuals of EBMI/EBMI, EBMI/Ebm and Ebmi/Ebmi genotype.

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2樓2010-11-14 12:46:59

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