 |
 |
 |
| 24小時(shí)熱門版塊排行榜 |
- >論壇更新日志 (4599)
- >考研 (902)
- >導(dǎo)師招生 (860)
- >文獻(xiàn)求助 (225)
- >休閑灌水 (102)
- >蟲(chóng)友互識(shí) (92)
- >考博 (86)
- >碩博家園 (70)
- >招聘信息布告欄 (67)
- >論文道賀祈福 (53)
- >基金申請(qǐng) (37)
- >博后之家 (29)
- >SciFinder/Reaxys (25)
- >論文投稿 (19)
- >綠色求助(高懸賞) (17)
- >找工作 (15)
 |
 |
 |
||
 |
 |
 |
 |
 |
| 9 | 1/1 | 返回列表 |
| 查看: 1403 | 回復(fù): 8 | |||
| 本帖產(chǎn)生 1 個(gè) 翻譯EPI ，點(diǎn)擊這里進(jìn)行查看 | |||
zxc578642銀蟲(chóng) (小有名氣)
|
[交流]
求助一段英語(yǔ)翻譯
|
||
|
A previous cLC method, involving large injection volumes (20 μL) and on-column focusing techniques, provided a suitable sensitivity using an Inertsil? C8 capillary column, DAD and gradient elution. This method showed that composition of the focusing solution, mobile phase and pH have remarkable effects on HA separation. In order to improve this separation, an isocratic cLC method compatible with MS detection was developed and the influence of several chromatographic factors on separation quality evaluated. Different reversed-phase packings such as Luna? C8, Luna? C18, Synergi? Max-RP (C12) and Synergi Fusion (C18 and polar embedded group) were tested using UV-DAD detection. These columns were chosen due to their high efficiencies and bonded phase surface coverage. Considering the different particle sizes, flow rate was set at 15 μL min?1 for both Synergi Fusion? and Synergi? Max-RP, 12 and 9 μL min?1 for Luna? C8 and Luna? C18 columns respectively. Chromatographic conditions were optimized using experimental design methodologies such as central composite design. Factors and ranges selected for the optimization of mobile phase composition were ACN (11.6–28.4%), ammonium acetate concentration (13.2–46.8 mM) and buffer pH (3.6–4.4). Response variables were expressed in terms of resolution, calculated at baseline, between the worst resolved peak pair NH–H (Rs,min), and retention time of the last eluting peak (tend). From the obtained equations, it could be concluded that Rs,min was significantly affected by both ACN percentage and pH while tend only by ACN. In addition, the ammonium acetate concentration had no significant effect on separation. Chromatographic separation efficiency was optimized for Rs,min ≥ 2 and minimum tend value. Luna? C8 capillary column provided poor resolution. Luna? C18 and Synergi? Max-RP columns provided similar Rs,min to Synergi? Fusion but higher efficiencies. However, Synergi? Max-RP column provided poor peak symmetry. Therefore, Luna? C18 column was selected for further optimization with the quadrupole MS detector. Taking into account the instrumental limitations of the MS capillary nebulizer, the ammonium acetate concentration in the mobile phase was fixed to 5 mM. Chromatographic separation was optimized using multifactorial design. Factors and ranges selected were ACN (12–18%) and pH (3.6–4.1). For the injection, buffered focusing solutions with 5% MeOH were used. Table 1 includes the values of the experimental responses (Rs,min and tend), which were fitted into the following normalized polynomial equations: (1)Rs,min=1.74?0.51pH?1.35ACN?0.06pH2+0.39pH ACN+0.40ACN2 (2)tend=12.18+0.18pH?11.02ACN+0.76pH2?0.94pH ACN+4.83 ACN2 Determination coefficients were 0.988 and 0.992 for Rs,min and tend respectively, showing the reliability of the equations. In the studied domain, both ACN percentage and pH affected significantly to Rs,min (p values 0.0000 and 0.0002 respectively) (Eq. (1)) while ACN (%) affected only tend (p value 0.0000 and 0.0005 for the single and quadratic term respectively) (Eq. (2)). As can be expected, the interactions between factors (ACN-pH) were significant only for Rs,min response (p = 0.0033). In Fig. 1 it can be observed that the ACN percentage decrease in the mobile phase increases Rs,min and tend. However, when pH decreases, Rs,min increases and tend does not change significantly. The chromatographic separation efficiency was optimized by maximizing Rs,min and minimizing tend values which maximized the desirability function over the selected region. The maximum desirability function yielded a tend = 13.6 min and Rs,min = 2.2 at pH 3.6 and 14.5% ACN. The estimated response surfaces predicted that Rs,min > 2 and tend in the range 12.5–15.0 min could be obtained at pH 3.6–3.7 and 13–15% ACN. As a compromise, pH 3.6 and 13% ACN were selected as optimum values. Under these conditions tend = 15.0 min and Rs,min = 2.3 were expected and experimentally assessed. Finally, ammonium formate 5 mM was also tested and selected to avoid problems into the capillary MS nebulizer. |
回復(fù)此樓» 猜你喜歡
【2026考研調(diào)劑】制藥工程 284分 求相關(guān)專業(yè)調(diào)劑名額
已經(jīng)有7人回復(fù)
-
334分 一志愿武理-080500 材料求調(diào)劑
已經(jīng)有3人回復(fù)
-
0854電子信息求調(diào)劑
已經(jīng)有9人回復(fù)
-
347求調(diào)劑
已經(jīng)有4人回復(fù)
-
0854人工智能方向招收調(diào)劑
已經(jīng)有4人回復(fù)
-
求b區(qū)院校調(diào)劑
已經(jīng)有4人回復(fù)
-
材料與化工考研調(diào)劑
已經(jīng)有9人回復(fù)
-
求調(diào)劑
已經(jīng)有3人回復(fù)
-
總分293求調(diào)劑
已經(jīng)有4人回復(fù)
-
284求調(diào)劑
已經(jīng)有15人回復(fù)
» 搶金幣啦！回帖就可以得到:
-
持續(xù)創(chuàng)業(yè)者，A8離異男征婚，事業(yè)遇到瓶頸，尋找創(chuàng)業(yè)生活伴侶
+1/455
-
課題組接收化學(xué)、材料科學(xué)與工程、材料工程及化學(xué)工程專業(yè)碩士研究生
+1/191
-
求助有機(jī)化學(xué)第二版陸陽(yáng)申?yáng)|升主編課件或者電子版書(shū)籍
+1/86
-
招收調(diào)劑生，動(dòng)物醫(yī)學(xué)專業(yè)
+1/84
-
招收化學(xué)，材料，環(huán)境，化工等專業(yè)基礎(chǔ)的同學(xué)攻讀碩士研究生
+1/84
-
江南大學(xué)招收具有3年海外博后經(jīng)歷的青年教師
+1/81
-
球差相關(guān)知識(shí)
+1/45
-
【分享】招收08工學(xué)碩士（要求過(guò)國(guó)家線）
+1/38
-
【211博士招生】環(huán)境化學(xué)、地學(xué)、毒理方向，擅長(zhǎng)污染物遷移轉(zhuǎn)化降解、計(jì)算模擬者優(yōu)先
+1/35
-
西華大學(xué)材料學(xué)院表面科學(xué)與工程技術(shù)科研團(tuán)隊(duì)2026年招收研究生
+1/34
-
福建師范大學(xué)閩江學(xué)者陳巍教授團(tuán)隊(duì)招收2026年化學(xué)、化工或材料專業(yè)的學(xué)碩6名
+1/31
-
雙一流大學(xué)湘潭大學(xué)“化工過(guò)程模擬與強(qiáng)化”國(guó)家地方聯(lián)合工程研究中心招收博士生
+1/23
-
春眠不覺(jué)曉
+1/21
-
下周見(jiàn)一個(gè)合作者，應(yīng)該問(wèn)些什么
+1/16
-
【2026調(diào)劑】南方科技大學(xué)-深圳理工大學(xué)-中科院 聯(lián)培086000免疫方向(26.9入學(xué))
+1/8
-
中國(guó)民航飛行學(xué)院先進(jìn)連接團(tuán)隊(duì)招收航空宇航、航空機(jī)械專業(yè)碩士研究生（學(xué)碩專碩均可）
+1/7
-
【第三輪招生】澳科大誠(chéng)招2026年秋季藥劑學(xué)碩士研究生（3月31日下午18:00截止）
+1/5
-
【博士招生】上海交通大學(xué)陳倩櫟課題組招收2026年申請(qǐng)-考核博士生1名（能源材料方向）
+1/5
-
上海師范大學(xué)有機(jī)化學(xué)專業(yè)研究生招生
+1/2
-
大連海事大學(xué)船舶潔凈能源研究中心2026年博士研究生招生啟事
+1/1
zft_1984
金蟲(chóng) (著名寫手)
- 應(yīng)助: 7 (幼兒園)
- 金幣: 21.6
- 帖子: 2532
- 在線: 274.5小時(shí)
- 蟲(chóng)號(hào): 735073
2樓2010-12-03 00:41:36
gary8812
鐵桿木蟲(chóng) (著名寫手)
- 翻譯EPI: 58
- 應(yīng)助: 11 (小學(xué)生)
- 貴賓: 0.005
- 金幣: 9278
- 帖子: 1781
- 在線: 1158.4小時(shí)
- 蟲(chóng)號(hào): 607349
3樓2010-12-03 18:43:09
petrocatal
木蟲(chóng) (正式寫手)
- 翻譯EPI: 134
- 應(yīng)助: 23 (小學(xué)生)
- 貴賓: 0.01
- 金幣: 3436.6
- 帖子: 959
- 在線: 299.5小時(shí)
- 蟲(chóng)號(hào): 557856
4樓2010-12-04 14:47:24
petrocatal
木蟲(chóng) (正式寫手)
- 翻譯EPI: 134
- 應(yīng)助: 23 (小學(xué)生)
- 貴賓: 0.01
- 金幣: 3436.6
- 帖子: 959
- 在線: 299.5小時(shí)
- 蟲(chóng)號(hào): 557856
5樓2010-12-07 16:12:49
ringzhu 
木蟲(chóng) (職業(yè)作家)
- 翻譯EPI: 24
- 應(yīng)助: 5 (幼兒園)
- 貴賓: 1.318
- 金幣: 1370.6
- 帖子: 4511
- 在線: 703.5小時(shí)
- 蟲(chóng)號(hào): 825134
6樓2010-12-07 16:14:54
petrocatal
木蟲(chóng) (正式寫手)
- 翻譯EPI: 134
- 應(yīng)助: 23 (小學(xué)生)
- 貴賓: 0.01
- 金幣: 3436.6
- 帖子: 959
- 在線: 299.5小時(shí)
- 蟲(chóng)號(hào): 557856
7樓2010-12-07 17:28:31
flower666666
銅蟲(chóng) (正式寫手)
- 翻譯EPI: 6
- 應(yīng)助: 0 (幼兒園)
- 金幣: 73.2
- 帖子: 315
- 在線: 57.3小時(shí)
- 蟲(chóng)號(hào): 881261
★ ★
ringzhu(金幣-2):本版嚴(yán)禁機(jī)器翻譯。。。。 2010-12-08 13:07:33
zxc578642(金幣+35, 翻譯EPI+1): 2010-12-11 00:08:34
ringzhu(金幣-2):本版嚴(yán)禁機(jī)器翻譯。。。。 2010-12-08 13:07:33
zxc578642(金幣+35, 翻譯EPI+1): 2010-12-11 00:08:34
|
先前的分類號(hào)方法，涉及到大注射量（20μL）和柱上聚焦技術(shù)，提供了合適的靈敏度使用Inertsil？ C8的毛細(xì)管柱，爸爸和梯度洗脫。這種方法表明，聚焦解決方案，流動(dòng)相組成和pH值對(duì)醫(yī)管局分離成效顯著。為了改善這種分離，一等度分類號(hào)兼容MS檢測(cè)法的開(kāi)發(fā)和幾種因素對(duì)色譜分離質(zhì)量的影響進(jìn)行評(píng)估。 不同的反相填料，如Luna？ C8的，Luna？ C18時(shí)，Synergi？馬克斯反相（十二）和Synergi融合（C18和極性嵌入組）進(jìn)行了測(cè)試使用紫外二極管陣列檢測(cè)。這些列被選為由于其高效率和鍵合相表面覆蓋�？紤]到不同顆粒大小，流量率定為15微升分鐘？Synergi融合為1？和Synergi？馬克斯余段，12分鐘和第9微升？負(fù)責(zé)盧納1？ C8和盧娜？ C18柱分別。 色譜條件進(jìn)行了優(yōu)化，采用中心組合設(shè)計(jì)，如實(shí)驗(yàn)設(shè)計(jì)方法。因素和流動(dòng)相組成的優(yōu)化選擇范圍為乙腈（11.6-28.4％），醋酸銨濃度（13.2-46.8毫米）和緩沖液pH值（3.6-4.4）。響應(yīng)變量中表達(dá)了在基準(zhǔn)計(jì)算的決議而言，最糟糕的解決高峰之間的配對(duì)的NH -的H（盧比，分鐘），并保留最后洗脫峰（傾向）的時(shí)間。從得到的方程，可以得出結(jié)論，盧比，最小顯著比例都乙腈和pH的影響，而往往只乙腈。此外，醋酸銨濃度無(wú)顯著影響的分離。 色譜分離效率進(jìn)行優(yōu)化盧比，≥2分和最低傾向于價(jià)值。盧納？ C8的毛細(xì)管柱提供的分辨率較低。盧納？ C18和Synergi？馬克斯反相柱提供了類似的盧比，分鐘，以Synergi？融合，但更高的效率。然而，Synergi？馬克斯窮人提供的反相柱峰的對(duì)稱性。因此，盧娜？ C18柱被選定為四極桿質(zhì)譜檢測(cè)器的進(jìn)一步優(yōu)化。 考慮到MS的毛細(xì)管霧化器的工具的限制，在流動(dòng)相中醋酸銨濃度為5毫米。色譜分離采用多因素進(jìn)行了優(yōu)化設(shè)計(jì)。被選定的因素和范圍乙腈（12-18％）和pH值（3.6-4.1）。對(duì)于注射液，緩沖聚焦5％甲醇被用來(lái)解決方案。 表1包括對(duì)實(shí)驗(yàn)的反應(yīng)值（盧比，min和靠攏），分為以下規(guī)范化多項(xiàng)式擬合方程： （1）盧比，最小= 1.74？0.51pH？1.35ACN？0.06pH2 0.39 pH值乙腈+0.40 ACN2 （2）= 12.18 +0.18 pH值趨向？11.02ACN 0.76 pH值為2？0.94pH乙腈4.83 ACN2 決定系數(shù)分別為盧比，最小為0.988和0.992，往往分別顯示了該方程的可靠性。在所研究的領(lǐng)域，都乙腈率和pH值的影響顯著盧比，分（P值分別為0.0000和0.0002）（式（1）），而乙腈（％）僅影響往往（P值0.0000和0.0005為單和二次任期分別）（式（2））。正如可以預(yù)料，因素之間（乙腈- pH值）有顯著的相互作用只有盧比，最小反應(yīng)（P = 0.0033）。在圖。 1可以觀察到，在增加流動(dòng)相乙腈比例下降盧比，min和趨向。然而，當(dāng)pH值降低，盧比，最小升幅，而且往往沒(méi)有明顯變化。 色譜分離效率的最大化盧比，min和價(jià)值觀趨于優(yōu)化，最大限度地減少對(duì)選定的區(qū)域最大化的可取性功能。最大的可取性功能產(chǎn)生了往往和Rs = 13.6分，3.6分，在pH = 2.2和14.5％乙腈。估計(jì)響應(yīng)面預(yù)測(cè)，遙感，分“2和范圍12.5-15.0分鐘往往可以在pH值3.6-3.7和13-15％乙腈獲得。作為一種妥協(xié)，pH值3.6和13％乙腈被選定為最佳值。在這種情況往往和Rs = 15.0分鐘，2.3分=預(yù)期和實(shí)驗(yàn)評(píng)估。最后，甲酸銨5毫米進(jìn)行了試驗(yàn)，并選擇了避免陷入毛細(xì)管質(zhì)譜霧化器的問(wèn)題。 |
8樓2010-12-08 10:42:12
gary8812
鐵桿木蟲(chóng) (著名寫手)
- 翻譯EPI: 58
- 應(yīng)助: 11 (小學(xué)生)
- 貴賓: 0.005
- 金幣: 9278
- 帖子: 1781
- 在線: 1158.4小時(shí)
- 蟲(chóng)號(hào): 607349
9樓2010-12-14 21:32:45
相關(guān)版塊跳轉(zhuǎn)
我要訂閱樓主 zxc578642 的主題更新
| 9 | 1/1 | 返回列表 |
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 285求調(diào)劑 +3 | AZMK 2026-03-24 | 3/150 |
|
|---|---|---|---|---|
|
[考研] 081700 調(diào)劑 267分 +10 | 迷人的哈哈 2026-03-23 | 10/500 |
|
|
|
[考研] 0854電子信息求調(diào)劑 324 +4 | Promise-jyl 2026-03-23 | 4/200 |
|
|
|
[考研] 材料調(diào)劑 +3 | iwinso 2026-03-23 | 3/150 |
|
|
|
[考研] 求調(diào)劑323材料與化工 +4 | 1124361 2026-03-24 | 4/200 |
|
|
|
[考研] 289求調(diào)劑 +9 | 懷瑾握瑜l 2026-03-20 | 9/450 |
|
|
|
[考研] 292求調(diào)劑 +4 | 鵝鵝鵝額額額額?/a> 2026-03-25 | 4/200 |
|
|
|
[有機(jī)交流]
 有機(jī)合成求助
20+3
|
FENGSHUJEI 2026-03-23 | 5/250 |
|
|
|
[考研] 招08考數(shù)學(xué) +7 | laoshidan 2026-03-20 | 16/800 |
|
|
|
[考研] 070300化學(xué)求調(diào)劑 +9 | 苑豆豆 2026-03-20 | 9/450 |
|
|
|
[考研] 291求調(diào)劑 +8 | hhhhxn.. 2026-03-23 | 8/400 |
|
|
|
[考研] 一志愿國(guó)科過(guò)程所081700，274求調(diào)劑 +3 | 三水研0水立方 2026-03-23 | 3/150 |
|
|
|
[考研] 328求調(diào)劑 +4 | LHHL66 2026-03-23 | 4/200 |
|
|
|
[考研] 一志愿華中科技大學(xué)071000，求調(diào)劑 +4 | 沿岸有貝殼6 2026-03-21 | 4/200 |
|
|
|
[考研] 材料學(xué)碩301分求調(diào)劑 +7 | Liyouyumairs 2026-03-21 | 7/350 |
|
|
|
[考研] 材料求調(diào)劑 +5 | @taotao 2026-03-21 | 5/250 |
|
|
|
[考研] 297求調(diào)劑 +3 | 喜歡還是不甘心 2026-03-20 | 3/150 |
|
|
|
[考研] 311求調(diào)劑 +3 | 勇敢的小吳 2026-03-20 | 3/150 |
|
|
|
[考研] 308求調(diào)劑 +3 | 阿姐阿姐家啊 2026-03-18 | 3/150 |
|
|
|
[考研] 一志愿西安交通大學(xué) 學(xué)碩 354求調(diào)劑211或者雙一流 +3 | 我想要讀研究生 2026-03-20 | 3/150 |
|