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請幫忙翻譯一個關(guān)于口蹄疫病毒的英文摘要
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A nucleotides fragment 2020VP1 related to T-cell epitope (aa21-40) and B-cell epitope (aa141-160) of VP1 of Food-and-mouth disease virus (FMDV)type O was synthesized, and the recombined expression vector r2020-LTB-2020-STI was correctly constructed, which was used to express the fusion protein consisted of two copies of aa21-40aa141-160 of FMDV VP1 and LTB and STI Enterotoxins of Escherichia coli. The fusion protein with molecular weight of about 45kD was successfully expressed in E.coli BL21(DE3) RIL at high level and confirmed by SDS-PAGE. The purified fusion protein could be specifically recognized by CTB antibody. The purified protein was then used to vaccinate guinea pigs, rabbits and Balb/c mice at 6-8 week age, and immune responses were finally observed. The fusion protein could induce proliferation of spleen T cells in vaccinated guinea pigs and elicit a high level of neutralizing antibody in rabbits. It could be concluded that the fusion protein could activate FMDV-specific cellular immune-response and humoral immune-response simultaneously. At the same time, the vaccinated mice could survive challenge of Enterotoxigenic Escherichia coli C83902, and the sera of vaccinated rabbits could neutralize the STI toxin. Moreover, the fusion protein had no STI toxicity, indicating that the fusion protein had LTB and STI immunogenicities. Therefore, it can be concluded that the fusion protein could be explored as a potentially efficient FMDV and ETEC vaccine. 謝謝翻譯帝們哈 [ Last edited by dianshineng on 2010-12-8 at 18:13 ] |
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| 合成了與FMDV病毒O型的VPI的T細(xì)胞單抗原決定簇(aa21-40)和B細(xì)胞單抗原決定簇相關(guān)的核苷酸片段,并正確構(gòu)建了重組表達(dá)載體 r2020-LTB-2020-STI ,它用來表達(dá)由兩套FMDV VP1和LTB 的 aa21-40aa141-160 和大腸桿菌的STI腸毒素構(gòu)成的融合蛋白。分子量約為45kD的融合蛋白在大腸桿菌E.coli BL21(DE3) RIL中成功高水平表達(dá)并通過SDS - PAGE驗(yàn)證。純化的融合蛋白能被CTB抗體特異識別。該融合蛋白能夠誘導(dǎo)免疫豚鼠的脾T細(xì)胞增殖,并在兔子中誘導(dǎo)出高水平的中和抗體?梢缘贸鲞@樣的結(jié)論:該融合蛋白能激活口蹄疫病毒特異性細(xì)胞免疫反應(yīng)和體液免疫反應(yīng)。同時,接種疫苗的老鼠在大腸桿菌腸毒素的挑戰(zhàn)下可以存活下來,接種疫苗的兔子的血清可中和STI毒素。 |
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